An Arabidopsis Secondary Metabolite Directly Targets Expression of the Bacterial Type III Secretion System to Inhibit Bacterial Virulence

Plants deploy a variety of secondary metabolites to fend off pathogen attack. Although defense compounds are generally considered toxic to microbes, the exact mechanisms are often unknown. Here, we show that the Arabidopsis defense compound sulforaphane (SFN) functions primarily by inhibiting Pseudo...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Cell host & microbe Ročník 27; číslo 4; s. 601
Hlavní autoři:	Wang, Wei, Yang, Jing, Zhang, Jian, Liu, Yong-Xin, Tian, Caiping, Qu, Baoyuan, Gao, Chulei, Xin, Peiyong, Cheng, Shujing, Zhang, Wenjing, Miao, Pei, Li, Lei, Zhang, Xiaojuan, Chu, Jinfang, Zuo, Jianru, Li, Jiayang, Bai, Yang, Lei, Xiaoguang, Zhou, Jian-Min
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 08.04.2020
Témata:	Arabidopsis - metabolism Bacterial Proteins - drug effects Cysteine - drug effects Cysteine - metabolism Disease Resistance Gene Expression Regulation, Bacterial Isothiocyanates - metabolism Isothiocyanates - pharmacology Plant Diseases - microbiology Pseudomonas syringae - drug effects Pseudomonas syringae - metabolism Secondary Metabolism Transcription Factors - drug effects Type III Secretion Systems - drug effects Type III Secretion Systems - genetics defense compound glucosinolate Arabidopsis Pseudomonas syringae Type III Secretion System
ISSN:	1934-6069, 1934-6069
On-line přístup:	Zjistit podrobnosti o přístupu
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Plants deploy a variety of secondary metabolites to fend off pathogen attack. Although defense compounds are generally considered toxic to microbes, the exact mechanisms are often unknown. Here, we show that the Arabidopsis defense compound sulforaphane (SFN) functions primarily by inhibiting Pseudomonas syringae type III secretion system (TTSS) genes, which are essential for pathogenesis. Plants lacking the aliphatic glucosinolate pathway, which do not accumulate SFN, were unable to attenuate TTSS gene expression and exhibited increased susceptibility to P. syringae strains that cannot detoxify SFN. Chemoproteomics analyses showed that SFN covalently modified the cysteine at position 209 of HrpS, a key transcription factor controlling TTSS gene expression. Site-directed mutagenesis and functional analyses further confirmed that Cys209 was responsible for bacterial sensitivity to SFN in vitro and sensitivity to plant defenses conferred by the aliphatic glucosinolate pathway. Collectively, these results illustrate a previously unknown mechanism by which plants disarm a pathogenic bacterium.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1934-6069 1934-6069
DOI:	10.1016/j.chom.2020.03.004