Cleaved Amplified Polymorphic Sequences (CAPS) Markers for Characterization of the LuFAD3A Gene from Various Flax (Linum usitatissimum L.) Cultivars

Depending on the variety, flax (Linum usitatissimum L.) provides an oil rich in omega-3 acids (especially 50% α-linolenic acid) with proven health properties, and is used as a raw industrial material. Alpha-linolenic acid is a polyunsaturated fatty acid easily subject to oxidative transformation. Th...

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Published in:Agronomy (Basel) Vol. 12; no. 6; p. 1432
Main Authors: Walkowiak, Magdalena, Matuszczak, Marcin, Spasibionek, Stanisław, Liersch, Alina, Mikołajczyk, Katarzyna
Format: Journal Article
Language:English
Published: Basel MDPI AG 01.06.2022
Subjects:
agronomy
alpha-linolenic acid
autoxidation
color
Cultivars
Desaturase
DNA
Enzymes
Exons
fatty acid composition
Fatty acids
Flax
Flowers & plants
Genes
Genetic testing
Genotypes
introns
leaves
Linolenic acid
linseed oil
Linum usitatissimum
Linum usitatissimum L
LuFAD3A
Markers
molecular genetics
Mutation
Nutritive value
Off flavor
off flavors
Oxidation
Plant tissues
Point mutation
Polymerization
Polyunsaturated fatty acids
Seeds
United Kingdom > UK
England
ISSN:2073-4395, 2073-4395
Online Access:Get full text
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Abstract Depending on the variety, flax (Linum usitatissimum L.) provides an oil rich in omega-3 acids (especially 50% α-linolenic acid) with proven health properties, and is used as a raw industrial material. Alpha-linolenic acid is a polyunsaturated fatty acid easily subject to oxidative transformation. The auto-oxidation of α-linolenic acid is the main process contributing to off-flavor, color loss, and change in the nutritional value of flax oil. We used six flax genotypes differing in fatty acid content in our research. For all the DNA samples extracted from the leaf tissue of the studied flax, the new, cleaved amplified polymorphic sequences (CAPS) markers specific to the LuFAD3A desaturase gene were applied. A specific PCR product from the LuFAD3A flax gene comprising fragments of exon 5, exon 6, and the intron between these exons was digested using six various restriction enzymes. These experiments could differentiate between some of the studied varieties of flax. We also proved that the LuFAD3A gene mutation previously detected by other authors in the low-linolenic forms of flax (solin line 593–708) was absent in the low-linolenic forms of flax that we studied (Linola KLA and Linola KLB).
AbstractList Depending on the variety, flax (Linum usitatissimum L.) provides an oil rich in omega-3 acids (especially 50% α-linolenic acid) with proven health properties, and is used as a raw industrial material. Alpha-linolenic acid is a polyunsaturated fatty acid easily subject to oxidative transformation. The auto-oxidation of α-linolenic acid is the main process contributing to off-flavor, color loss, and change in the nutritional value of flax oil. We used six flax genotypes differing in fatty acid content in our research. For all the DNA samples extracted from the leaf tissue of the studied flax, the new, cleaved amplified polymorphic sequences (CAPS) markers specific to the LuFAD3A desaturase gene were applied. A specific PCR product from the LuFAD3A flax gene comprising fragments of exon 5, exon 6, and the intron between these exons was digested using six various restriction enzymes. These experiments could differentiate between some of the studied varieties of flax. We also proved that the LuFAD3A gene mutation previously detected by other authors in the low-linolenic forms of flax (solin line 593–708) was absent in the low-linolenic forms of flax that we studied (Linola KLA and Linola KLB).
Author Matuszczak, Marcin
Liersch, Alina
Spasibionek, Stanisław
Mikołajczyk, Katarzyna
Walkowiak, Magdalena
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CitedBy_id crossref_primary_10_34248_bsengineering_1432978
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10.1046/j.1365-313X.1993.04020403.x
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10.4141/cjps91-054
10.1111/j.1365-313X.2006.02802.x
ContentType Journal Article
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Copyright_xml – notice: 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
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Snippet Depending on the variety, flax (Linum usitatissimum L.) provides an oil rich in omega-3 acids (especially 50% α-linolenic acid) with proven health properties,...
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StartPage 1432
SubjectTerms agronomy
alpha-linolenic acid
autoxidation
color
Cultivars
Desaturase
DNA
Enzymes
Exons
fatty acid composition
Fatty acids
Flax
Flowers & plants
Genes
Genetic testing
Genotypes
introns
leaves
Linolenic acid
linseed oil
Linum usitatissimum
Linum usitatissimum L
LuFAD3A
Markers
molecular genetics
Mutation
Nutritive value
Off flavor
off flavors
Oxidation
Plant tissues
Point mutation
Polymerization
Polyunsaturated fatty acids
Seeds
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Title Cleaved Amplified Polymorphic Sequences (CAPS) Markers for Characterization of the LuFAD3A Gene from Various Flax (Linum usitatissimum L.) Cultivars
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