Biospecific labeling with undecagold: visualization of the biotin-binding site on avidin

The biotin-binding site on avidin has been labeled with biotin conjugated to undecagold, an organometallic cluster compound containing 11 gold atoms in a core angestroms in diameter. Examination of unstained specimens by scanning transmission electron microscopy reveals the labeled sites directly, w...

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Vydáno v:Science (American Association for the Advancement of Science) Ročník 218; číslo 4569; s. 290
Hlavní autoři: Safer, D, Hainfeld, J, Wall, J S, Reardon, J E
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 15.10.1982
Témata:
Avidin - metabolism
Binding Sites
Biotin - metabolism
Gold - metabolism
Organogold Compounds
Organometallic Compounds - metabolism
Ovalbumin - analogs & derivatives
ISSN:0036-8075
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Popis
Shrnutí:The biotin-binding site on avidin has been labeled with biotin conjugated to undecagold, an organometallic cluster compound containing 11 gold atoms in a core angestroms in diameter. Examination of unstained specimens by scanning transmission electron microscopy reveals the labeled sites directly, without computational averaging or filtering of the images. This approach should be widely applicable for determining the locations of subunits and functional site in biological macromolecules at a resolution at a resolution in range of 15 angstroms.
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ISSN:0036-8075
DOI:10.1126/science.7123234