Drug Resistance and Molecular Characteristics of Mycobacterium tuberculosis: A Single Center Experience

In recent years, the incidence of tuberculosis (TB) and mortality caused by the disease have been decreasing. However, the number of drug-resistant tuberculosis patients is increasing rapidly year by year. Here, a total of 380 Mycobacterium tuberculosis (MTB)-positive formalin-fixed and paraffin-emb...

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Vydáno v:Journal of personalized medicine Ročník 12; číslo 12; s. 2088
Hlavní autoři: Li, Shanshan, Chen, Wen, Feng, Mengru, Liu, Yuejiao, Wang, Fenghua
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 19.12.2022
MDPI
Témata:
Drug resistance
Mutation
Mutation rates
Mycobacterium tuberculosis
Paraffin
Pathology
Precision medicine
RpoB protein
RpsL gene
Sex differences
Tuberculosis
United States > US
China
Germany
molecular characteristic
Mycobacterium tuberculosis
drug-resistant
gene mutation
PCR
ISSN:2075-4426, 2075-4426
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Shrnutí:In recent years, the incidence of tuberculosis (TB) and mortality caused by the disease have been decreasing. However, the number of drug-resistant tuberculosis patients is increasing rapidly year by year. Here, a total of 380 Mycobacterium tuberculosis (MTB)-positive formalin-fixed and paraffin-embedded tissue (FFPE) specimens diagnosed in the Department of Pathology of the Eighth Medical Center, Chinese PLA General Hospital were collected. Among 380 cases of MTB, 85 (22.37%) were susceptible to four anti-TB drugs and the remaining 295 (77.63%) were resistant to one or more drugs. The rate of MDR-TB was higher in previously treated cases (52.53%) than in new cases [(36.65%), p < 0.05]. Of previously treated cases, the rate of drug resistance was higher in females than in males (p < 0.05). Among specimens obtained from males, the rate of drug resistance was higher in new cases than in previously treated cases (p < 0.05). Of mutation in drug resistance-related genes, the majority (53/380, 13.95%) of rpoB gene carried the D516V mutation, and 13.42% (51/380) featured mutations in both the katG and inhA genes. Among the total specimens, 18.68% (71/380) carried the 88 M mutation in the rpsL gene, and the embB gene focused on the 306 M2 mutation with a mutation rate of 19.74%. Among the resistant INH, the mutation rate of −15 M was higher in resistance to more than one drug than in monodrug-resistant (p < 0.05). In conclusion, the drug resistance of MTB is still very severe and the timely detection of drug resistance is conducive to the precise treatment of TB.
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These authors contributed equally to this work.
ISSN:2075-4426
2075-4426
DOI:10.3390/jpm12122088