Protocol for elucidating metabolite binding and regulation of TET2 dioxygenase
Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the e...
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| Veröffentlicht in: | STAR protocols Jg. 6; H. 3; S. 104015 |
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| Abstract | Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation.
For complete details on the use and execution of this protocol, please refer to Cheng et al.1
[Display omitted]
•Instructions for the purification of highly active, tag-free human TET2CD protein•Procedures for flow cytometry-based detection of TET2 activity in vitro•Guidance on the simultaneous screening of TET2 regulatory metabolites•Steps for STD NMR-based detection of metabolite-TET2 interactions
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. |
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| AbstractList | Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. For complete details on the use and execution of this protocol, please refer to Cheng et al.
. Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. For complete details on the use and execution of this protocol, please refer to Cheng et al.1 •Instructions for the purification of highly active, tag-free human TET2CD protein•Procedures for flow cytometry-based detection of TET2 activity in vitro•Guidance on the simultaneous screening of TET2 regulatory metabolites•Steps for STD NMR-based detection of metabolite-TET2 interactions Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. For complete details on the use and execution of this protocol, please refer to Cheng et al.1.Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. For complete details on the use and execution of this protocol, please refer to Cheng et al.1. Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. For complete details on the use and execution of this protocol, please refer to Cheng et al.1 [Display omitted] •Instructions for the purification of highly active, tag-free human TET2CD protein•Procedures for flow cytometry-based detection of TET2 activity in vitro•Guidance on the simultaneous screening of TET2 regulatory metabolites•Steps for STD NMR-based detection of metabolite-TET2 interactions Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Epigenetic enzyme activity is coupled to cellular metabolism through their reliance on metabolic cofactors and substrates. Here, we describe steps for combining biochemical assays and saturation transfer difference (STD) NMR spectroscopy to experimentally validate metabolite binding and assess the effect on TET2 activity. This protocol enables the identification of both TET2 activators and inhibitors, providing a framework for studying the interplay between metabolism and epigenetic regulation. |
| ArticleNumber | 104015 |
| Author | Cheng, Zhou-Li Zhang, Shuyuan Ye, Dan |
| Author_xml | – sequence: 1 givenname: Shuyuan surname: Zhang fullname: Zhang, Shuyuan organization: Huashan Hospital, Fudan University, and International Co-laboratory of Medical Epigenetics and Metabolism of Ministry of Science and Technology, and Key Laboratory of Metabolism and Molecular Medicine of Ministry of Education, and Molecular and Cell Biology Lab, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China – sequence: 2 givenname: Zhou-Li surname: Cheng fullname: Cheng, Zhou-Li organization: Huashan Hospital, Fudan University, and International Co-laboratory of Medical Epigenetics and Metabolism of Ministry of Science and Technology, and Key Laboratory of Metabolism and Molecular Medicine of Ministry of Education, and Molecular and Cell Biology Lab, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China – sequence: 3 givenname: Dan surname: Ye fullname: Ye, Dan email: yedan@fudan.edu.cn organization: Huashan Hospital, Fudan University, and International Co-laboratory of Medical Epigenetics and Metabolism of Ministry of Science and Technology, and Key Laboratory of Metabolism and Molecular Medicine of Ministry of Education, and Molecular and Cell Biology Lab, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China |
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| Cites_doi | 10.1021/ed101169t 10.1021/acschembio.3c00619 10.1016/j.cell.2013.11.020 10.1038/s41580-018-0029-7 10.1016/j.cmet.2012.06.001 10.1016/j.drudis.2011.12.016 10.1038/nrg.2017.33 10.1016/j.trecan.2017.12.005 10.1016/j.ccell.2018.07.014 10.1021/ja4028346 10.1016/j.cmet.2025.01.019 |
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| Keywords | Protein Biochemistry NMR Molecular Biology Protein expression and purification Cell Biology |
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| SubjectTerms | Cell Biology Dioxygenases - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Epigenesis, Genetic Humans Magnetic Resonance Spectroscopy - methods Molecular Biology NMR Protein Binding Protein Biochemistry Protein expression and purification Proto-Oncogene Proteins - metabolism Protocol |
| Title | Protocol for elucidating metabolite binding and regulation of TET2 dioxygenase |
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