Key Considerations on CITE‐Seq for Single‐Cell Multiomics

ABSTRACT CITE‐Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is an advanced single‐cell sequencing method to profile both gene expression and protein abundance simultaneously in individual cells using single‐cell mRNA sequencing techniques alongside antibody‐derived tags (ADTs)...

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Bibliographic Details
Published in:Proteomics (Weinheim) Vol. 25; no. 21-22; pp. 206 - 213
Main Authors: Song, Hye‐Wong, Martin, Jody, Shi, Xiaoshan, Tyznik, Aaron J.
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01.11.2025
Subjects:
Animals
antibody‐derived tags
Biological activity
cellular indexing of transcriptomes and epitopes by sequencing
Data analysis
Epitopes
Epitopes - genetics
Gene expression
Gene Expression Profiling - methods
Gene sequencing
High-Throughput Nucleotide Sequencing - methods
Humans
library preparation
Multiomics
next‐generation sequencing
Proteins
proteogenomics
Proteome - genetics
Proteomes
Proteomics - methods
Sequence Analysis, RNA - methods
Single-Cell Analysis - methods
Transcriptome
Transcriptomes
Workflow
antibody‐derived tags
proteogenomics
library preparation
next‐generation sequencing
cellular indexing of transcriptomes and epitopes by sequencing
ISSN:1615-9853, 1615-9861, 1615-9861
Online Access:Get full text
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Summary:ABSTRACT CITE‐Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is an advanced single‐cell sequencing method to profile both gene expression and protein abundance simultaneously in individual cells using single‐cell mRNA sequencing techniques alongside antibody‐derived tags (ADTs), for protein detection. The characterization of both the transcriptome and the proteome from the same cells provides a powerful and multiomic approach for understanding the mechanisms of complex biological processes. This review focuses on the workflow of CITE‐Seq using a microwell‐based single‐cell analysis system as an example and provides key considerations for staining cells with ADTs. By highlighting critical information for CITE‐Seq library preparation, sequencing, and data analysis, this review provides a practical guide with which to perform comprehensive CITE‐Seq workflow.
Bibliography:Hye‐Wong Song and Jody Martin contributed equally to this work.
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ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.202400011