Kaempferol-induces vasorelaxation via endothelium-independent pathways in rat isolated pulmonary artery

Kaempferol, a flavonoid, is the essential part of human diet. Flavonoids have different pharmacological activities like cardioprotective, anti-inflammatory and anti-oxidant. The aim of current study was to investigate vasorelaxant potential of kaempferol on rat isolated pulmonary artery and to asses...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Pharmacological reports Ročník 70; číslo 5; s. 863 - 874
Hlavní autori: Mahobiya, Archana, Singh, Thakur Uttam, Rungsung, Soya, Kumar, Tarun, Chandrasekaran, Gokul, Parida, Subhashree, Kumar, Dinesh
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Cham Elsevier B.V 01.10.2018
Springer International Publishing
Predmet:
Animals
Apamin - pharmacology
Barium Compounds - pharmacology
Calcium Chloride - antagonists & inhibitors
Chlorides - pharmacology
Dose-Response Relationship, Drug
Drug Safety and Pharmacovigilance
Endothelium
Endothelium, Vascular - drug effects
Estradiol - analogs & derivatives
Estradiol - pharmacology
Fulvestrant
Glyburide - pharmacology
In Vitro Techniques
Indomethacin - pharmacology
Isoquinolines - pharmacology
Kaempferol
Kaempferols - antagonists & inhibitors
Kaempferols - pharmacology
Male
Morpholines - pharmacology
NG-Nitroarginine Methyl Ester - pharmacology
Original Article
Oxadiazoles - pharmacology
Peptides - pharmacology
Pharmacotherapy
Pharmacy
Potassium - pharmacology
Pulmonary artery
Pulmonary Artery - drug effects
Pulmonary Artery - physiology
Pyrroles - pharmacology
Quinoxalines - pharmacology
Rat
Rats
Sulfonamides - pharmacology
Tetraethylammonium - pharmacology
Vasodilation - drug effects
Vasodilator Agents - antagonists & inhibitors
Vasodilator Agents - pharmacology
Rat
Kaempferol
Pulmonary artery
Endothelium
ISSN:1734-1140, 2299-5684, 2299-5684
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Kaempferol, a flavonoid, is the essential part of human diet. Flavonoids have different pharmacological activities like cardioprotective, anti-inflammatory and anti-oxidant. The aim of current study was to investigate vasorelaxant potential of kaempferol on rat isolated pulmonary artery and to assess the underling mechanisms. Tension experiments were conducted on both the branches of main pulmonary artery of rats. Experiments were done using isolated organ bath system by recording tension with the help of data acquisition system, Power Lab. Kaempferol (10−8–10−4.5M) caused concentration-dependent relaxation (Emax 124.33±4.37%; pD2 5.03±0.084) of endothelium-intact pulmonary artery. In endothelium-denuded arterial rings, relaxation produced by kaempferol was not different from intact artery. L-NAME, indomethacin, combination of L-NAME and indomethacin did not show any effect on kaempferol-induced relaxation. Kaempferol-induced relaxation was reduced (Emax 55.53±7.72%) in 60mMK+ pre-contracted pulmonary arterial rings. Iberiotoxin significantly decreased (Emax 71.68±11.84%) the relaxation response. However, glibenclamide, BaCl2, 4-AP (1mM) and ICI182780 did not reduce the kaempferol-induced relaxation. TEA (10mM) and 4-AP (5mM) significantly reduced relaxation. Kaempferol-induced relaxation was significantly attenuated (Emax 94.92±19.60%) in presence of ODQ. H89 significantly decreased (Emax, 98.38±8.55%) the kaempferol-induced relaxation in rat pulmonary arterial rings. HC067047 and apamin did not show any effect on kaempferol-induced relaxation. In endothelium-denuded K+ (80mM)-depolarized arterial rings, kaempferol (10μM) markedly reduced CaCl2-induced contractions (Emax 35.14±6.53% vs. control 69.04±15.19%). Kaempferol relaxes rat pulmonary artery in endothelium-independent manner through involvement of BKCa channel, sGC, PKA pathways and inhibition of Ca2+-influx through L-type calcium channels.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1734-1140
2299-5684
2299-5684
DOI:10.1016/j.pharep.2018.03.006