Yeast Expression Systems: Overview and Recent Advances

Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropria...

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Bibliographic Details
Published in:Molecular biotechnology Vol. 61; no. 5; pp. 365 - 384
Main Authors: Baghban, Roghayyeh, Farajnia, Safar, Rajabibazl, Masoumeh, Ghasemi, Younes, Mafi, AmirAli, Hoseinpoor, Reyhaneh, Rahbarnia, Leila, Aria, Maryam
Format: Journal Article
Language:English
Published: New York Springer US 01.05.2019
Springer Nature B.V
Subjects:
Baking yeast
Biochemistry
Biological Techniques
Biotechnology
Blastobotrys adeninivorans
Cell Biology
Chemistry
Chemistry and Materials Science
Fermentation
Hansenula polymorpha
hosts
Human Genetics
Kluyveromyces lactis
Kluyveromyces marxianus var. lactis
Komagataella pastoris
Pichia pastoris
Protein Science
Proteins
recombinant proteins
Review
Saccharomyces cerevisiae
Schizosaccharomyces pombe
Yarrowia lipolytica
Yeast
yeasts
Expression system
Pichia pastoris
Hansenula polymorpha
Saccharomyces cerevisiae
Yarrowia lipolytica
ISSN:1073-6085, 1559-0305, 1559-0305
Online Access:Get full text
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Summary:Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Currently, several new systems are commercially available; some of them are private and need licensing. The potential for secretory expression of heterologous proteins in yeast proposed this system as a candidate for the production of complex eukaryotic proteins. The common yeast expression hosts used for recombinant proteins’ expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis , and Schizosaccharomyces pombe . This review is dedicated to discuss on significant characteristics of the most common methylotrophic and non-methylotrophic yeast expression systems with an emphasis on their advantages and new developments.
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ISSN:1073-6085
1559-0305
1559-0305
DOI:10.1007/s12033-019-00164-8