Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects

Plant transformation based on Agrobacterium- mediated transformation is a technique that mimics the natural agrobacterium system for gene(s) introduction into crops. Through this technique, various crop species have been improved/modified for different trait/s, showing a successful genetic transform...

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Veröffentlicht in:Molecular biotechnology Jg. 66; H. 8; S. 1836 - 1852
Hauptverfasser: Rahman, Saleem Ur, Khan, Muhammad Omar, Ullah, Rahim, Ahmad, Fayaz, Raza, Ghulam
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York Springer US 01.08.2024
Springer Nature B.V
Schlagworte:
Agrobacterium
Agrobacterium - genetics
Biochemistry
Biological Techniques
Biotechnology
Cell Biology
Chemistry
Chemistry and Materials Science
cost effectiveness
CRISPR
CRISPR-Cas Systems
Crops
Crops, Agricultural - genetics
Crops, Agricultural - growth & development
Gene Editing - methods
Genetic Engineering - methods
Genetic transformation
genetically modified organisms
Genome editing
Human Genetics
Introduced species
Plant bacterial diseases
Plants, Genetically Modified - genetics
Plants, Genetically Modified - growth & development
Protein Science
Review Paper
Transformation, Genetic
Transgenes
Transgenic plants
Genetic Engineering
CRISPR/Cas9
mediated transformation
Transgenic crops
Agrobacterium-mediated transformation
ISSN:1073-6085, 1559-0305, 1559-0305
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:Plant transformation based on Agrobacterium- mediated transformation is a technique that mimics the natural agrobacterium system for gene(s) introduction into crops. Through this technique, various crop species have been improved/modified for different trait/s, showing a successful genetic transformation so far. This technique has many advantages over other transformation methods such as stable integration of transgene, cost effective. However, there are many limitations of this technology such as mostly the crops are recalcitrant to agrobacterium, low transformation efficiency, transgene integration as well as off targets. So, it’s very important to explore the major limitations and possible solutions for Agrobacterium- mediated transformation in order to increase its genetic transformation efficiency. Therefore, the present review article gives a comprehensive study how the transgenic crops are developed using Agrobacterium -mediated transformation, crops that have already been modified through this method, and risks associated with transgenic plants based on Agrobacterium- mediated transformation. Moreover, the challenges and problems associated with Agrobacterium -mediated transformation and how those problems can be solved in future for a successful genetic transformation of crops using modern biotechnology techniques such as CRISPR/Cas9 systems. The present review article will be really helpful for the audience those working on Genome editing of crops using Agrobacterium- mediated transformation and will opens many ways for future plant genetic transformation.
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ISSN:1073-6085
1559-0305
1559-0305
DOI:10.1007/s12033-023-00826-8