Identification of structural DNA variations in human cell cultures after long-term passage

Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells dem...

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Vydané v:Cell cycle (Georgetown, Tex.) Ročník 14; číslo 2; s. 200 - 205
Hlavní autori: Pavlova, GV, Vergun, AA, Rybalkina, EY, Butovskaya, PR, Ryskov, AP
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Taylor & Francis 17.01.2015
Predmet:
Adipose Tissue - cytology
Cells, Cultured
Chromosomal Instability
DNA - genetics
DNA - isolation & purification
DNA - metabolism
DNA Primers - metabolism
Humans
Polymorphism, Single Nucleotide
Random Amplified Polymorphic DNA Technique
Stromal Cells - cytology
Stromal Cells - metabolism
Random amplified polymorphic DNA (RAPD)
mutations
Cell cultures
DNA stability in cells
different number of passages
ISSN:1538-4101, 1551-4005, 1551-4005
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Shrnutí:Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different number of passages were cloned and sequenced. The chromosomal localization of these fragments was identified and single-nucleotide variations in RAPD fragments isolated from cell lines after 8-12 passages were revealed. Some of them had permanent localization, while most variations demonstrated random distribution and can be considered as de novo mutations.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1538-4101
1551-4005
1551-4005
DOI:10.4161/15384101.2014.974427