Isolation of a coronavirus during studies on puffinosis, a disease of the Manx shearwater (Puffinus puffinus)

A virus was isolated from 2 day-old mice inoculated with homogenates of either the lungs or blood of 2 different shearwaters affected by puffinosis. Examination of infected suckling mouse brain and infected NCTC-1469 (mouse liver) cell cultures, by electron microscopy, revealed virus particles and i...

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Bibliographic Details
Published in:Archives of virology Vol. 73; no. 1; p. 1
Main Authors: Nuttall, P A, Harrap, K A
Format: Journal Article
Language:English
Published: Austria 01.01.1982
Subjects:
Animals
Antibodies, Viral - analysis
Bird Diseases - microbiology
Birds
Brain - microbiology
Cell Line
Chick Embryo
Coronaviridae - growth & development
Coronaviridae - isolation & purification
Coronaviridae - ultrastructure
Cytopathogenic Effect, Viral
Inclusion Bodies, Viral - ultrastructure
Liver - microbiology
Lung - microbiology
Mice
Viremia - veterinary
ISSN:0304-8608
Online Access:Get more information
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Summary:A virus was isolated from 2 day-old mice inoculated with homogenates of either the lungs or blood of 2 different shearwaters affected by puffinosis. Examination of infected suckling mouse brain and infected NCTC-1469 (mouse liver) cell cultures, by electron microscopy, revealed virus particles and inclusion bodies characteristic of a coronavirus. Neutralization, complement fixation and fluorescent antibody tests showed that the virus was related to mouse hepatitis virus. The virus was not isolated from 445 control, uninfected mice. Neutralising antibodies were not detected in 39 sera from diseased shearwaters and 2 sera from apparently healthy birds. Two shearwaters inoculated with the virus did not develop clinical signs of infection. The question of whether the virus was isolated from shearwaters or from laboratory mice is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0304-8608
DOI:10.1007/BF01341722