In situ abundance and carbon fixation activity of distinct anoxygenic phototrophs in the stratified seawater lake Rogoznica

Summary Sulphide‐driven anoxygenic photosynthesis is an ancient microbial metabolism that contributes significantly to inorganic carbon fixation in stratified, sulphidic water bodies. Methods commonly applied to quantify inorganic carbon fixation by anoxygenic phototrophs, however, cannot resolve th...

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Bibliographic Details
Published in:Environmental microbiology Vol. 21; no. 10; pp. 3896 - 3908
Main Authors: Pjevac, Petra, Dyksma, Stefan, Goldhammer, Tobias, Mujakić, Izabela, Koblížek, Michal, Mußmann, Marc, Amann, Rudolf, Orlić, Sandi
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2019
Wiley Subscription Services, Inc
Subjects:
Abundance
autotrophs
Bacteria
Carbon
Carbon Cycle
carbon dioxide fixation
Carbon fixation
Chemical analysis
Chemocline
Chlorobium - isolation & purification
Chlorobium - metabolism
Chlorobium phaeobacteroides
Chromatiaceae - isolation & purification
Chromatiaceae - metabolism
Croatia
Flow cytometry
Forests
genome
Genomes
Green sulfur bacteria
Halochromatium
Heterotrophic growth
Inorganic carbon
Labeling
Lakes
Lakes - microbiology
Metabolism
metagenomics
Microorganisms
Photopigments
Photosynthesis
Populations
Radioisotopes
radiolabeling
Seawater
Seawater - microbiology
Sulfides
Sulfides - metabolism
Sulfur
Sulfur - metabolism
Sulphides
Sulphur
surface water
Water analysis
Workflow
Croatia
ISSN:1462-2912, 1462-2920, 1462-2920
Online Access:Get full text
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Summary:Summary Sulphide‐driven anoxygenic photosynthesis is an ancient microbial metabolism that contributes significantly to inorganic carbon fixation in stratified, sulphidic water bodies. Methods commonly applied to quantify inorganic carbon fixation by anoxygenic phototrophs, however, cannot resolve the contributions of distinct microbial populations to the overall process. We implemented a straightforward workflow, consisting of radioisotope labelling and flow cytometric cell sorting based on the distinct autofluorescence of bacterial photopigments, to discriminate and quantify contributions of co‐occurring anoxygenic phototrophic populations to in situ inorganic carbon fixation in environmental samples. This allowed us to assign 89.3% ± 7.6% of daytime inorganic carbon fixation by anoxygenic phototrophs in Lake Rogoznica (Croatia) to an abundant chemocline‐dwelling population of green sulphur bacteria (dominated by Chlorobium phaeobacteroides), whereas the co‐occurring purple sulphur bacteria (Halochromatium sp.) contributed only 1.8% ± 1.4%. Furthermore, we obtained two metagenome assembled genomes of green sulphur bacteria and one of a purple sulphur bacterium which provides the first genomic insights into the genus Halochromatium, confirming its high metabolic flexibility and physiological potential for mixo‐ and heterotrophic growth.
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ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/1462-2920.14739