Polysaccharides from green sweet pepper increase the antineoplastic effect of methotrexate on mammary tumor cells

This study investigated the antineoplastic effects and toxicity of long-term treatment with polysaccharides from sweet green pepper (Capsicum annuum [CAP]), and concomitant treatment with CAP + methotrexate (MTX) on mammary tumor cells in vivo and in vitro. Ehrlich tumor cells were subcutaneously in...

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Veröffentlicht in:International journal of biological macromolecules Jg. 158; S. 1071 - 1081
Hauptverfasser: Adami, Eliana Rezende, Corso, Claudia Rita, Turin-Oliveira, Natalia Mulinari, Galindo, Claudia Martins, Milani, Leticia, Stipp, Maria Carolina, da Silva, Liziane Cristine Malaquias, do Nascimento, Georgia Erdmann, Chaves, Pedro Felipe Pereira, Chequin, Andressa, Mariott, Marihá, da Silva, Luisa Mota, Klassen, Giseli, Ramos, Edneia A.S., Cordeiro, Lucimara M.C., Acco, Alexandra
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.09.2020
Schlagworte:
angiogenesis
Breast tumor
Capsicum annuum
cell viability
drug therapy
females
gene expression
human cell lines
inflammation
interleukin-10
interleukin-4
interleukin-6
Interleukins
laboratory animals
mammary neoplasms (animal)
methotrexate
mice
necrosis
neoplasm cells
polysaccharides
sweet peppers
toxicity
tumor necrosis factor-alpha
Interleukins
Capsicum annuum
Breast tumor
ISSN:0141-8130, 1879-0003, 1879-0003
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Zusammenfassung:This study investigated the antineoplastic effects and toxicity of long-term treatment with polysaccharides from sweet green pepper (Capsicum annuum [CAP]), and concomitant treatment with CAP + methotrexate (MTX) on mammary tumor cells in vivo and in vitro. Ehrlich tumor cells were subcutaneously inoculated in female Swiss mice. The long-term treatment (31 days) with CAP (100 mg kg−1, p.o.) reduced the tumor growth and did not induce toxicity. The combined treatment protocol of 100 mg kg−1 CAP (p.o.) + 1 mg kg−1 MTX (i.p.) for 21 days inhibited the tumor growth in 95%, higher than the inhibition induced by MTX alone (1.0 or 2.5 mg kg−1, i.p.). In tumors, both CAP and CAP + MTX decreased the gene expression of Vegf, vessel area, and IL-4 and IL-10 levels, and increased IL-6 levels and the degree of necrosis. Treatment with CAP + MTX also increased TNF-α levels in tumors. Additionally, CAP + MTX treatment reduced the viability of human MDA-MB-231 and MDA-MB-436 mammary tumor cells in culture. In fact, CAP exerted antineoplastic effects in vivo and in vitro against mammary tumor cells, possibly by modulating inflammation and angiogenesis. CAP may be a promising adjunct chemotherapy with lower toxicity. •Long treatment with polysaccharides from sweet green pepper (CAP) has antineoplastic effect.•CAP and CAP + methotrexate (MTX) treatment modulate inflammation and angiogenesis in tumor microenvironment.•CAP increases the effects of MTX in mammary tumor cells from human and mice.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2020.05.001