Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody

•A sensitive immunocytochemical (IC) and immunohistochemical (IHC) method has been developed for detection of African swine fever (ASF) virus.•The IC test has the same sensitivity as the indirect fluorescent antibody test method using polyclonal antibodies.•The IHC method detects ASF virus in all ti...

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Vydáno v:	Journal of virological methods Ročník 282; s. 113886
Hlavní autoři:	Szeredi, Levente, Bakcsa, Erika, Zádori, Zoltán, Mészáros, István, Olasz, Ferenc, Bálint, Ádám, Locsmándi, Gabriella, Erdélyi, Károly
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.08.2020
Témata:	African swine fever African swine fever virus Asia Cell culture coat proteins Europe Immunocytochemistry Immunohistochemistry mice monoclonal antibodies viruses Wild boar wild boars Asia Europe Immunohistochemistry Cell culture African swine fever Immunocytochemistry Wild boar
ISSN:	0166-0934, 1879-0984, 1879-0984
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Abstract	•A sensitive immunocytochemical (IC) and immunohistochemical (IHC) method has been developed for detection of African swine fever (ASF) virus.•The IC test has the same sensitivity as the indirect fluorescent antibody test method using polyclonal antibodies.•The IHC method detects ASF virus in all tissue samples examined from wild boar cases of acute ASF disease.•ASF virus can be detected with the IHC method in tissue samples showing advanced autolytic changes. Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF.
AbstractList	Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF.Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF. •A sensitive immunocytochemical (IC) and immunohistochemical (IHC) method has been developed for detection of African swine fever (ASF) virus.•The IC test has the same sensitivity as the indirect fluorescent antibody test method using polyclonal antibodies.•The IHC method detects ASF virus in all tissue samples examined from wild boar cases of acute ASF disease.•ASF virus can be detected with the IHC method in tissue samples showing advanced autolytic changes. Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF. Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF.
ArticleNumber	113886
Author	Olasz, Ferenc Zádori, Zoltán Bálint, Ádám Locsmándi, Gabriella Szeredi, Levente Mészáros, István Erdélyi, Károly Bakcsa, Erika
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Keywords	Immunohistochemistry Cell culture African swine fever Immunocytochemistry Wild boar
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Snippet	•A sensitive immunocytochemical (IC) and immunohistochemical (IHC) method has been developed for detection of African swine fever (ASF) virus.•The IC test has... Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a...
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SubjectTerms	African swine fever African swine fever virus Asia Cell culture coat proteins Europe Immunocytochemistry Immunohistochemistry mice monoclonal antibodies viruses Wild boar wild boars
Title	Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody
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