Enhanced accumulation of important bioproducts in Chlorella vulgaris through AGPase gene silencing coupled with polyethylene glycol treatment

Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris s...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Journal of biotechnology Ročník 403; s. 81 - 92
Hlavní autoři:	Saha, Sumedha, Xaxa, Delrin Shaina, Ghosh, Sudip K., Maiti, Mrinal K.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.07.2025
Témata:	acetates AGPase biobased products biorefining biosynthesis biotechnology carbohydrate content carbon Carotenoids Chlorella vulgaris Chlorella vulgaris - drug effects Chlorella vulgaris - enzymology Chlorella vulgaris - genetics Chlorella vulgaris - growth & development Chlorella vulgaris - metabolism coproducts culture media exopolysaccharides fuel production Gene Silencing genes genetically modified organisms glucose-1-phosphate adenylyltransferase Glucose-1-Phosphate Adenylyltransferase - genetics Glucose-1-Phosphate Adenylyltransferase - metabolism lipid content liquid state fermentation metabolites microalgae PEG 6000 phosphates polyethylene glycol Polyethylene Glycols - chemistry Polyethylene Glycols - pharmacology RNA Interference RNA interference, starch biosynthesis starch Starch - biosynthesis Starch - metabolism AGPase PEG 6000 RNA interference, starch biosynthesis Carotenoids Chlorella vulgaris
ISSN:	0168-1656, 1873-4863, 1873-4863
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris strain more suitable for biorefinery by downregulating the ADP-glucose pyrophosphorylase (AGPase) enzyme involved in starch biosynthesis. Transgenic lines of C. vulgaris generated through RNA interference (RNAi) demonstrated diminished starch content with an average of 6.7 % (dry cell weight, DCW) from 10 % (DCW) in the untransformed control, after 5 days of shake flask culture in tris acetate phosphate medium with 16 h:8 h light:dark cycle. Under the same growth condition, the total carbohydrate content decreased by an average of 23.5 %, while the lipid content and carotenoid level improved by an average of 19.3 % and 23 %, respectively, in the RNAi lines compared to the control. RNAi lines showed a higher yield of other important co-products, like protein and exopolysaccharide by an average of 30.6 % and 19.6 %, respectively, compared to the control. Interestingly, when polyethylene glycol (PEG) 6000 (0.5 % w/v) was supplemented in the culture medium of C. vulgaris grown till mid-log phase, RNAi lines along with the untransformed control exhibited enhanced level of valuable metabolites after 7 days of PEG treatment. The average carotenoid content of 25.5 μg/mg was recorded in PEG-treated RNAi lines compared to 15.34 μg/mg in PEG-untreated untransformed control alga. Under the similar growth condition, the average lipid content increased to 22.5 % (DCW) in PEG-treated RNAi lines compared to 16.28 % (DCW) in PEG-untreated untransformed control. Overall, the study encompasses use of genetic engineering tool in combination with the application of biochemical modulator PEG to divert the carbon flux from starch biosynthesis towards improved production of important metabolites in microalga. •AGPase silencing decreased starch content with improved lipid and carotenoid levels.•AGPase downregulation enhanced protein and exopolysaccharide contents.•PEG treatment on RNAi lines positively impacted carotenoid and lipid contents.
AbstractList	Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris strain more suitable for biorefinery by downregulating the ADP-glucose pyrophosphorylase (AGPase) enzyme involved in starch biosynthesis. Transgenic lines of C. vulgaris generated through RNA interference (RNAi) demonstrated diminished starch content with an average of 6.7% (dry cell weight, DCW) from 10% (DCW) in the untransformed control, after 5 days of shake flask culture in tris acetate phosphate medium with 16h:8h light:dark cycle. Under the same growth condition, the total carbohydrate content decreased by an average of 23.5%, while the lipid content and carotenoid level improved by an average of 19.3% and 23%, respectively, in the RNAi lines compared to the control. RNAi lines showed a higher yield of other important co-products, like protein and exopolysaccharide by an average 30.6% and 19.6%, respectively, compared to the control. Interestingly, when polyethylene glycol (PEG) 6000 (0.5% w/v) was supplemented in the culture medium of C. vulgaris grown till mid-log phase, RNAi lines along with the untransformed control exhibited enhanced level of valuable metabolites after 7 days of PEG treatment. The average carotenoid content of 25.5μg/mg was recorded in PEG-treated RNAi lines compared to 15.34μg/mg in PEG-untreated untransformed control alga. Under the similar growth condition, the average lipid content increased to 22.5% (DCW) in PEG-treated RNAi lines compared to 16.28% (DCW) in PEG-untreated untransformed control. Overall, the study encompasses use of genetic engineering tool in combination with the application of biochemical modulator PEG to divert the carbon flux from starch biosynthesis towards improved production of important metabolites in microalga.Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris strain more suitable for biorefinery by downregulating the ADP-glucose pyrophosphorylase (AGPase) enzyme involved in starch biosynthesis. Transgenic lines of C. vulgaris generated through RNA interference (RNAi) demonstrated diminished starch content with an average of 6.7% (dry cell weight, DCW) from 10% (DCW) in the untransformed control, after 5 days of shake flask culture in tris acetate phosphate medium with 16h:8h light:dark cycle. Under the same growth condition, the total carbohydrate content decreased by an average of 23.5%, while the lipid content and carotenoid level improved by an average of 19.3% and 23%, respectively, in the RNAi lines compared to the control. RNAi lines showed a higher yield of other important co-products, like protein and exopolysaccharide by an average 30.6% and 19.6%, respectively, compared to the control. Interestingly, when polyethylene glycol (PEG) 6000 (0.5% w/v) was supplemented in the culture medium of C. vulgaris grown till mid-log phase, RNAi lines along with the untransformed control exhibited enhanced level of valuable metabolites after 7 days of PEG treatment. The average carotenoid content of 25.5μg/mg was recorded in PEG-treated RNAi lines compared to 15.34μg/mg in PEG-untreated untransformed control alga. Under the similar growth condition, the average lipid content increased to 22.5% (DCW) in PEG-treated RNAi lines compared to 16.28% (DCW) in PEG-untreated untransformed control. Overall, the study encompasses use of genetic engineering tool in combination with the application of biochemical modulator PEG to divert the carbon flux from starch biosynthesis towards improved production of important metabolites in microalga. Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris strain more suitable for biorefinery by downregulating the ADP-glucose pyrophosphorylase (AGPase) enzyme involved in starch biosynthesis. Transgenic lines of C. vulgaris generated through RNA interference (RNAi) demonstrated diminished starch content with an average of 6.7 % (dry cell weight, DCW) from 10 % (DCW) in the untransformed control, after 5 days of shake flask culture in tris acetate phosphate medium with 16 h:8 h light:dark cycle. Under the same growth condition, the total carbohydrate content decreased by an average of 23.5 %, while the lipid content and carotenoid level improved by an average of 19.3 % and 23 %, respectively, in the RNAi lines compared to the control. RNAi lines showed a higher yield of other important co-products, like protein and exopolysaccharide by an average of 30.6 % and 19.6 %, respectively, compared to the control. Interestingly, when polyethylene glycol (PEG) 6000 (0.5 % w/v) was supplemented in the culture medium of C. vulgaris grown till mid-log phase, RNAi lines along with the untransformed control exhibited enhanced level of valuable metabolites after 7 days of PEG treatment. The average carotenoid content of 25.5 μg/mg was recorded in PEG-treated RNAi lines compared to 15.34 μg/mg in PEG-untreated untransformed control alga. Under the similar growth condition, the average lipid content increased to 22.5 % (DCW) in PEG-treated RNAi lines compared to 16.28 % (DCW) in PEG-untreated untransformed control. Overall, the study encompasses use of genetic engineering tool in combination with the application of biochemical modulator PEG to divert the carbon flux from starch biosynthesis towards improved production of important metabolites in microalga. •AGPase silencing decreased starch content with improved lipid and carotenoid levels.•AGPase downregulation enhanced protein and exopolysaccharide contents.•PEG treatment on RNAi lines positively impacted carotenoid and lipid contents. Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for biofuel production and extraction of valuable co-products. This work focuses on the development of an industrially viable Chlorella vulgaris strain more suitable for biorefinery by downregulating the ADP-glucose pyrophosphorylase (AGPase) enzyme involved in starch biosynthesis. Transgenic lines of C. vulgaris generated through RNA interference (RNAi) demonstrated diminished starch content with an average of 6.7 % (dry cell weight, DCW) from 10 % (DCW) in the untransformed control, after 5 days of shake flask culture in tris acetate phosphate medium with 16 h:8 h light:dark cycle. Under the same growth condition, the total carbohydrate content decreased by an average of 23.5 %, while the lipid content and carotenoid level improved by an average of 19.3 % and 23 %, respectively, in the RNAi lines compared to the control. RNAi lines showed a higher yield of other important co-products, like protein and exopolysaccharide by an average of 30.6 % and 19.6 %, respectively, compared to the control. Interestingly, when polyethylene glycol (PEG) 6000 (0.5 % w/v) was supplemented in the culture medium of C. vulgaris grown till mid-log phase, RNAi lines along with the untransformed control exhibited enhanced level of valuable metabolites after 7 days of PEG treatment. The average carotenoid content of 25.5 μg/mg was recorded in PEG-treated RNAi lines compared to 15.34 μg/mg in PEG-untreated untransformed control alga. Under the similar growth condition, the average lipid content increased to 22.5 % (DCW) in PEG-treated RNAi lines compared to 16.28 % (DCW) in PEG-untreated untransformed control. Overall, the study encompasses use of genetic engineering tool in combination with the application of biochemical modulator PEG to divert the carbon flux from starch biosynthesis towards improved production of important metabolites in microalga.
Author	Ghosh, Sudip K. Xaxa, Delrin Shaina Maiti, Mrinal K. Saha, Sumedha
Author_xml	– sequence: 1 givenname: Sumedha surname: Saha fullname: Saha, Sumedha organization: Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, India – sequence: 2 givenname: Delrin Shaina surname: Xaxa fullname: Xaxa, Delrin Shaina organization: Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India – sequence: 3 givenname: Sudip K. surname: Ghosh fullname: Ghosh, Sudip K. organization: Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, India – sequence: 4 givenname: Mrinal K. orcidid: 0000-0001-7803-4356 surname: Maiti fullname: Maiti, Mrinal K. email: maitimk@bt.iitkgp.ac.in, mrinalkmaiti@gmail.com organization: Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, India
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/40222633$$D View this record in MEDLINE/PubMed
BookMark	eNqFkc1uEzEUhS1URNPCI4C8ZDPBP_PjEQtURaUgVYIFrC3PnZsZRx472J6iPATvzISkGzZZ3c13ztE954Zc-eCRkLecrTnj9YfdetfZkBHWgolqzco1Y80LsuKqkUWpanlFVgunCl5X9TW5SWnHGCvbir8i1yUTQtRSrsifez8aD9hTAzBPszPZBk_DltppH2I2PtMlZx9DP0NO1Hq6GV2I6JyhT7MbTLSJ5jGGeRjp3cN3k5AO6JEm69CD9QOFMO_dkvDb5pHugztgHg_uyAzuAMHRHNHkCX1-TV5ujUv45nxvyc_P9z82X4rHbw9fN3ePBZSM5WLbKcPAsEb2nTGyxa4pa6g5dKC6uupK0dcgDHSyEg0qtXRiQLWilI1qe1nJW_L-5Lv89WvGlPVkExx_8hjmpKVgteC8beRllKu25ZWq2gV9d0bnbsJe76OdTDzo57YX4OMJgBhSirjVYPO_wnM01mnO9HFbvdPnbfVxW81KvWy7qKv_1M8Bl3SfTjpcGn2yGHUCi8fNbUTIug_2gsNfbnXEcw
CitedBy_id	crossref_primary_10_1016_j_algal_2025_104313 crossref_primary_10_3390_membranes15070205
Cites_doi	10.1016/j.copbio.2010.03.005 10.4141/cjps-2014-110 10.1101/2022.08.30.505872 10.1074/jbc.271.27.16281 10.1016/S1046-2023(03)00036-7 10.1139/o59-099 10.1021/je201197m 10.1007/s11274-024-04074-z 10.1038/s42003-021-01976-8 10.1007/s10811-005-1641-4 10.1073/pnas.0903478106 10.1038/s41598-018-30809-8 10.1071/CH02052 10.1111/j.1469-8137.1970.tb02446.x 10.1039/C7FO00409E 10.1016/j.biortech.2019.121894 10.1080/10408398.2018.1432561 10.1002/bit.22807 10.1104/pp.86.4.1131 10.1016/j.ijbiomac.2020.10.159 10.1016/S0021-9258(19)35399-2 10.1186/s12934-023-02244-6 10.1016/j.algal.2017.07.027 10.1016/j.biortech.2021.124870 10.1111/j.1399-3054.2012.01603.x 10.1021/ac60111a017 10.1089/ind.2013.0039 10.1186/s13068-016-0671-2 10.1016/j.scitotenv.2020.137598 10.1111/j.1399-3054.2000.1100407.x 10.1002/j.1460-2075.1992.tb05167.x 10.1002/bit.22033 10.1080/09553002.2020.1741717 10.1186/s13068-018-1334-2 10.3389/fpls.2019.01444 10.1128/JB.183.3.1069-1077.2001 10.1002/bit.23016 10.1007/s00709-020-01570-5 10.1104/pp.95.3.716 10.1111/j.1529-8817.1968.tb04667.x 10.1073/pnas.54.6.1665 10.1007/s00344-012-9268-6 10.1021/ac60147a030 10.1007/s00572-003-0228-7 10.1016/j.biortech.2020.123562 10.21273/JASHS03944-16 10.3390/plants9111440 10.1104/pp.93.2.785 10.1111/pbi.12664 10.1046/j.1365-313X.2001.01105.x 10.1016/j.ymben.2010.02.002 10.1007/s11802-016-2797-z 10.1007/s00449-014-1139-z 10.1186/s40104-021-00593-z 10.1016/j.bcab.2018.09.011 10.1002/er.8069 10.1126/science.133.3463.1486 10.1111/pbi.12131 10.3390/md20050336 10.1002/1873-3468.13198 10.1111/j.1440-1835.2006.00416.x 10.1093/mutage/17.3.261 10.1002/anie.200902672 10.1104/pp.92.4.881
ContentType	Journal Article
Copyright	2025 Elsevier B.V. Copyright © 2025 Elsevier B.V. All rights reserved.
Copyright_xml	– notice: 2025 Elsevier B.V. – notice: Copyright © 2025 Elsevier B.V. All rights reserved.
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 7S9 L.6
DOI	10.1016/j.jbiotec.2025.04.007
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic AGRICOLA AGRICOLA - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic AGRICOLA AGRICOLA - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE AGRICOLA
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering
EISSN	1873-4863
EndPage	92
ExternalDocumentID	40222633 10_1016_j_jbiotec_2025_04_007 S016816562500094X
Genre	Journal Article
GroupedDBID	--- --K --M -~X .GJ .~1 0R~ 1B1 1RT 1~. 1~5 29K 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM 9JN AAAJQ AABNK AAEDT AAEDW AAHBH AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AATTM AAXKI AAXUO AAYWO ABDPE ABFNM ABFRF ABGSF ABJNI ABMAC ABNUV ABUDA ABWVN ABXDB ACDAQ ACGFO ACGFS ACIUM ACRLP ACRPL ACVFH ADBBV ADCNI ADEWK ADEZE ADMUD ADNMO ADUVX ADVLN AEBSH AEFWE AEHWI AEIPS AEKER AENEX AEUPX AFJKZ AFPUW AFTJW AFXIZ AGCQF AGEKW AGHFR AGQPQ AGRDE AGRNS AGUBO AGYEJ AHHHB AHPOS AIEXJ AIGII AIIUN AIKHN AITUG AKBMS AKRWK AKURH AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU APXCP ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CJTIS CNWQP CS3 D-I DU5 EBS EFJIC EJD ENUVR EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HMG HVGLF HZ~ IHE J1W KOM LUGTX LX3 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG RNS ROL RPZ SBG SDF SDG SDP SES SEW SIN SPC SPCBC SSG SSH SSI SSU SSZ T5K WUQ XPP Y6R ZMT ~02 ~G- ~KM 9DU AAYXX ACLOT CITATION EFKBS EFLBG ~HD CGR CUY CVF ECM EIF NPM 7X8 7S9 L.6
ID	FETCH-LOGICAL-c400t-fb8a0ca073dbaa39eb746c61cbc8b65b42d6c2acb3527e88187ac89243789d353
ISICitedReferencesCount	2
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001471943500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	0168-1656 1873-4863
IngestDate	Thu Oct 02 22:59:06 EDT 2025 Fri Oct 03 00:23:43 EDT 2025 Mon Jul 21 05:47:43 EDT 2025 Sat Nov 29 07:57:33 EST 2025 Tue Nov 18 20:52:02 EST 2025 Sat Jun 07 17:01:53 EDT 2025
IsPeerReviewed	true
IsScholarly	true
Keywords	AGPase PEG 6000 RNA interference, starch biosynthesis Carotenoids Chlorella vulgaris
Language	English
License	Copyright © 2025 Elsevier B.V. All rights reserved.
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c400t-fb8a0ca073dbaa39eb746c61cbc8b65b42d6c2acb3527e88187ac89243789d353
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ORCID	0000-0001-7803-4356
PMID	40222633
PQID	3189915859
PQPubID	23479
PageCount	12
ParticipantIDs	proquest_miscellaneous_3206211973 proquest_miscellaneous_3189915859 pubmed_primary_40222633 crossref_citationtrail_10_1016_j_jbiotec_2025_04_007 crossref_primary_10_1016_j_jbiotec_2025_04_007 elsevier_sciencedirect_doi_10_1016_j_jbiotec_2025_04_007
PublicationCentury	2000
PublicationDate	2025-07-01
PublicationDateYYYYMMDD	2025-07-01
PublicationDate_xml	– month: 07 year: 2025 text: 2025-07-01 day: 01
PublicationDecade	2020
PublicationPlace	Netherlands
PublicationPlace_xml	– name: Netherlands
PublicationTitle	Journal of biotechnology
PublicationTitleAlternate	J Biotechnol
PublicationYear	2025
Publisher	Elsevier B.V
Publisher_xml	– name: Elsevier B.V
References	Jnandabhiram, Sailen Prasad (bib24) 2012 Na, Aryal, Fatihi, Kang, Lu (bib45) 2018; 11 Matos, Cardoso, Bandarra, N, Afonso (bib41) 2017; 8 Rodolfi, Chini Zittelli, Bassi, Padovani, Biondi, Bonini, Tredici (bib50) 2009; 102 Allen (bib1) 1968; 4 Lichtenthaler (bib36) 1987; 148 Van den Koornhuyse, Libessart, Delrue, Zabawinski, Decq, Iglesias, Carton, Preiss, Ball (bib60) 1996; 271 Morell, Bloom, Preiss (bib43) 1988; 263 Yang, Sheng, Duan, Liang, Liang, Liu (bib66) 2012; 31 Kwak, Park, Shin, Koh, Lee, Jeong, Chang (bib27) 2017; 26 Okita, Nakata, Anderson, Sowokinos, Morell, Preiss (bib46) 1990; 93 Li, Han, Hu, Sommerfeld, Hu (bib34) 2010; 107 Tan, Lee (bib58) 2016; 9 Chandra, Mallick (bib9) 2022; 46 Ramazanov, Ramazanov (bib48) 2006; 54 Udayan, Pandey, Sirohi, Sreekumar, Sang, Sim, Kim, Pandey (bib59) 2022 Kato, Oyama, Inokuma, Vavricka, Matsuda, Hidese, Satoh, Oono, Chang, Hasunuma, Kondo (bib25) 2021; 4 Laroche (bib29) 2022; 20 Azoulay-Shemer, Schwankl, Rog, Moshelion, Schroeder (bib3) 2018; 592 Brányiková, Maršálková, Doucha, Brányik, Bišová, Zachleder, Vítová (bib8) 2011; 108 Chen, Khalili, Cairney (bib10) 2003; 13 Zabawinski, Van DenKoornhuyse, D’Hulst, Schlichting, Giersch, Delrue, Lacroix, Preiss, Ball (bib68) 2001; 183 Ball (bib4) 2002; 55 Sivaramakrishnan, Suresh, Pugazhendhi, Mercy Nisha Pauline, Incharoensakdi (bib55) 2020; 312 Maia, da, Cardoso, Mastrantonio, da, Bierhals, Moreira, Costa, Morais (bib39) 2020; 165 . Vanhercke, El Tahchy, Liu, Zhou, Shrestha, Divi, Ral, Mansour, Nichols, James, Horn, Chapman, Beaudoin, Ruiz-López, Larkin, de Feyter, Singh, Petrie (bib61) 2014; 12 DuBois, Gilles, Hamilton, Rebers, Smith (bib14) 1956; 28 Sulpice, Pyl, Ishihara, Trenkamp, Steinfath, Witucka-Wall, Gibon, Usadel, Poree, Piques, Von Korff, Steinhauser, Keurentjes, Guenther, Hoehne, Selbig, Fernie, Altmann, Stitt (bib57) 2009; 106 Barbarino, Lourenço (bib5) 2005; 17 Gorman, Levine (bib18) 1965; 54 Seng, Wu, Liang, Zhang, Yang, He, Yi (bib54) 2017; 142 Lagerwerff, Ogata, Eagle (bib28) 1961; 133 Preiss, Danner, Summers, Morell, Barton, Yang, Nieder (bib47) 1990; 92 Hohl, Schopfer (bib21) 1991; 95 Song, Xi, Sun (bib56) 2016; 15 Härtl, Kalinowski, Hoffmann, Preuss, Schwab (bib19) 2017; 15 Xu, Vanhercke, Shrestha, Luo, Akbar, Konik-Rose, Venugoban, Hussain, Tian, Singh, Li, Sharp, Liu (bib64) 2019; 10 Wesley, Helliwell, Smith, Wang, Rouse, Liu, Gooding, Singh, Abbott, Stoutjesdijk, Robinson, Gleave, Green, Waterhouse (bib62) 2001; 27 Lin, Caspar, Somerville, Preiss (bib37) 1988; 86 Biondi, Motta, Mosesso (bib6) 2002; 17 Eilam, Khattib, Pintel, Avni (bib15) 2023; 7 Lawlor (bib30) 1970; 69 Bligh, Dyer (bib7) 1959; 37 Müller-Röber, Sonnewald, Willmitzer (bib44) 1992; 11 Helliwell, Waterhouse (bib20) 2003; 30 Ambati, Gogisetty, Aswathanarayana, Ravi, Bikkina, Bo, Yuepeng (bib2) 2019; 59 Saha, Maji, Ghosh, Maiti (bib52) 2024; 40 Zhang, Wang, Li, Tan, Tao, Su, Xu, Tian, Xiong (bib69) 2021; 258 Xavier, J., Yadav, R.M., & Subramanyam, R. (2022). Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress (p. 2022.08.30.505872). bioRxiv. Yuan, Wang, Li, Li, Tai (bib67) 2014; 94 Fukuda, Hirasawa, Takemura, Takahashi, Chokshi, Pancha, Tanaka, Imamura (bib16) 2018; 8 Li, Han, Hu, Dauvillee, Sommerfeld, Ball, Hu (bib33) 2010; 12 Ran, Wang, Liu, Qi, Xiang, Yao, Zhang, Lan (bib49) 2019; 291 Scott, Davey, Dennis, Horst, Howe, Lea-Smith, Smith (bib53) 2010; 21 Lee, Yeh (bib31) 2014; 37 Miller (bib42) 1959; 31 Doyle, Doyle (bib13) 1990; 12 Maltsev, Kulikovskiy, Maltseva (bib40) 2023; 22 Saadaoui, Rasheed, Aguilar, Cherif, Al Jabri, Sayadi, Manning (bib51) 2021; 12 Daneshvar, Sik Ok, Tavakoli, Sarkar, Shaheen, Hong, Luo, Rinklebe, Song, Bhatnagar (bib11) 2021; 329 Li, Ye, Chen, Qi (bib35) 2012; 57 Liu, Zhang, Pu, Liu, Li, Zhao, Qin (bib38) 2014; 2014 Ismaiel, El-Ayouty, Said, Fathey (bib23) 2018; 16 Yang, Ma, Liang, Wei, Liang, Liu, Liu (bib65) 2012; 146 Gibon, Sulpice, Larher (bib17) 2000; 110 Knop, Hoogenboom, Fischer, Schubert (bib26) 2010; 49 Hosseini Tafreshi, Aghaie, Toghyani, Ramazani-Moghaddam-Arani (bib22) 2020; 96 Leu, Boussiba (bib32) 2014; 10 Din, Ibrahim, Farag, El-Gawad, El-Banhawy, Alaraidh, Rashad, Lashin, El-Yazied, Elkelish, Elbar (bib12) 2020; 9 Zhu, Cheng, Xu, Lu, Wang, Li, Yang (bib70) 2020; 720 Morell (10.1016/j.jbiotec.2025.04.007_bib43) 1988; 263 Li (10.1016/j.jbiotec.2025.04.007_bib34) 2010; 107 Saadaoui (10.1016/j.jbiotec.2025.04.007_bib51) 2021; 12 Li (10.1016/j.jbiotec.2025.04.007_bib35) 2012; 57 Sulpice (10.1016/j.jbiotec.2025.04.007_bib57) 2009; 106 Yang (10.1016/j.jbiotec.2025.04.007_bib66) 2012; 31 Vanhercke (10.1016/j.jbiotec.2025.04.007_bib61) 2014; 12 Matos (10.1016/j.jbiotec.2025.04.007_bib41) 2017; 8 Lagerwerff (10.1016/j.jbiotec.2025.04.007_bib28) 1961; 133 10.1016/j.jbiotec.2025.04.007_bib63 Udayan (10.1016/j.jbiotec.2025.04.007_bib59) 2022 Lichtenthaler (10.1016/j.jbiotec.2025.04.007_bib36) 1987; 148 Brányiková (10.1016/j.jbiotec.2025.04.007_bib8) 2011; 108 Lee (10.1016/j.jbiotec.2025.04.007_bib31) 2014; 37 Xu (10.1016/j.jbiotec.2025.04.007_bib64) 2019; 10 Maia (10.1016/j.jbiotec.2025.04.007_bib39) 2020; 165 Rodolfi (10.1016/j.jbiotec.2025.04.007_bib50) 2009; 102 Jnandabhiram (10.1016/j.jbiotec.2025.04.007_bib24) 2012 Müller-Röber (10.1016/j.jbiotec.2025.04.007_bib44) 1992; 11 Van den Koornhuyse (10.1016/j.jbiotec.2025.04.007_bib60) 1996; 271 Chandra (10.1016/j.jbiotec.2025.04.007_bib9) 2022; 46 Song (10.1016/j.jbiotec.2025.04.007_bib56) 2016; 15 Liu (10.1016/j.jbiotec.2025.04.007_bib38) 2014; 2014 Maltsev (10.1016/j.jbiotec.2025.04.007_bib40) 2023; 22 Eilam (10.1016/j.jbiotec.2025.04.007_bib15) 2023; 7 Ran (10.1016/j.jbiotec.2025.04.007_bib49) 2019; 291 Bligh (10.1016/j.jbiotec.2025.04.007_bib7) 1959; 37 Laroche (10.1016/j.jbiotec.2025.04.007_bib29) 2022; 20 Daneshvar (10.1016/j.jbiotec.2025.04.007_bib11) 2021; 329 Ball (10.1016/j.jbiotec.2025.04.007_bib4) 2002; 55 Gibon (10.1016/j.jbiotec.2025.04.007_bib17) 2000; 110 Ismaiel (10.1016/j.jbiotec.2025.04.007_bib23) 2018; 16 Zhang (10.1016/j.jbiotec.2025.04.007_bib69) 2021; 258 Kato (10.1016/j.jbiotec.2025.04.007_bib25) 2021; 4 Fukuda (10.1016/j.jbiotec.2025.04.007_bib16) 2018; 8 Yang (10.1016/j.jbiotec.2025.04.007_bib65) 2012; 146 Gorman (10.1016/j.jbiotec.2025.04.007_bib18) 1965; 54 Allen (10.1016/j.jbiotec.2025.04.007_bib1) 1968; 4 Chen (10.1016/j.jbiotec.2025.04.007_bib10) 2003; 13 Kwak (10.1016/j.jbiotec.2025.04.007_bib27) 2017; 26 Zhu (10.1016/j.jbiotec.2025.04.007_bib70) 2020; 720 Tan (10.1016/j.jbiotec.2025.04.007_bib58) 2016; 9 Doyle (10.1016/j.jbiotec.2025.04.007_bib13) 1990; 12 Scott (10.1016/j.jbiotec.2025.04.007_bib53) 2010; 21 Wesley (10.1016/j.jbiotec.2025.04.007_bib62) 2001; 27 DuBois (10.1016/j.jbiotec.2025.04.007_bib14) 1956; 28 Okita (10.1016/j.jbiotec.2025.04.007_bib46) 1990; 93 Preiss (10.1016/j.jbiotec.2025.04.007_bib47) 1990; 92 Yuan (10.1016/j.jbiotec.2025.04.007_bib67) 2014; 94 Härtl (10.1016/j.jbiotec.2025.04.007_bib19) 2017; 15 Na (10.1016/j.jbiotec.2025.04.007_bib45) 2018; 11 Leu (10.1016/j.jbiotec.2025.04.007_bib32) 2014; 10 Knop (10.1016/j.jbiotec.2025.04.007_bib26) 2010; 49 Ramazanov (10.1016/j.jbiotec.2025.04.007_bib48) 2006; 54 Miller (10.1016/j.jbiotec.2025.04.007_bib42) 1959; 31 Sivaramakrishnan (10.1016/j.jbiotec.2025.04.007_bib55) 2020; 312 Din (10.1016/j.jbiotec.2025.04.007_bib12) 2020; 9 Biondi (10.1016/j.jbiotec.2025.04.007_bib6) 2002; 17 Lin (10.1016/j.jbiotec.2025.04.007_bib37) 1988; 86 Seng (10.1016/j.jbiotec.2025.04.007_bib54) 2017; 142 Hosseini Tafreshi (10.1016/j.jbiotec.2025.04.007_bib22) 2020; 96 Saha (10.1016/j.jbiotec.2025.04.007_bib52) 2024; 40 Ambati (10.1016/j.jbiotec.2025.04.007_bib2) 2019; 59 Hohl (10.1016/j.jbiotec.2025.04.007_bib21) 1991; 95 Lawlor (10.1016/j.jbiotec.2025.04.007_bib30) 1970; 69 Barbarino (10.1016/j.jbiotec.2025.04.007_bib5) 2005; 17 Azoulay-Shemer (10.1016/j.jbiotec.2025.04.007_bib3) 2018; 592 Li (10.1016/j.jbiotec.2025.04.007_bib33) 2010; 12 Helliwell (10.1016/j.jbiotec.2025.04.007_bib20) 2003; 30 Zabawinski (10.1016/j.jbiotec.2025.04.007_bib68) 2001; 183
References_xml	– volume: 40 start-page: 271 year: 2024 ident: bib52 article-title: Engineered publication-title: World J. Microbiol. Biotechnol. – year: 2012 ident: bib24 article-title: Water Stress Effects on Leaf Growth and Chlorophyll Content but Not the Grain Yield in Traditional Rice ( publication-title: Am. J. Plant Sci. – volume: 2014 year: 2014 ident: bib38 article-title: Cloning and expression of a cytosolic HSP90 Gene in publication-title: BioMed. Res. Int. – volume: 93 start-page: 785 year: 1990 end-page: 790 ident: bib46 article-title: The subunit structure of potato tuber ADPglucose pyrophosphorylase 1 publication-title: Plant Physiol. – volume: 59 start-page: 1880 year: 2019 end-page: 1902 ident: bib2 article-title: Industrial potential of carotenoid pigments from microalgae: current trends and future prospects publication-title: Crit. Rev. Food Sci. Nutr. – volume: 12 start-page: 13 year: 1990 end-page: 15 ident: bib13 article-title: Isolation of plant DNA from fresh tissue publication-title: Focus – volume: 27 start-page: 581 year: 2001 end-page: 590 ident: bib62 article-title: Construct design for efficient, effective and high-throughput gene silencing in plants publication-title: Plant J.: Cell Mol. Biol. – volume: 20 start-page: 336 year: 2022 ident: bib29 article-title: Exopolysaccharides from microalgae and cyanobacteria: diversity of strains, production strategies, and applications publication-title: Mar. Drugs – volume: 12 start-page: 76 year: 2021 ident: bib51 article-title: Microalgal-based feed: promising alternative feedstocks for livestock and poultry production publication-title: J. Anim. Sci. Biotechnol. – volume: 16 start-page: 378 year: 2018 end-page: 384 ident: bib23 article-title: Transformation of publication-title: Biocatal. Agric. Biotechnol. – volume: 13 start-page: 173 year: 2003 end-page: 176 ident: bib10 article-title: Influence of water stress on biomass production by isolates of an ericoid mycorrhizal endophyte of publication-title: Mycorrhiza – volume: 31 start-page: 426 year: 1959 end-page: 428 ident: bib42 article-title: Use of dinitrosalicylic acid reagent for determination of reducing sugar publication-title: Anal. Chem. – volume: 107 start-page: 258 year: 2010 end-page: 268 ident: bib34 article-title: Inhibition of starch synthesis results in overproduction of lipids in publication-title: Biotechnol. Bioeng. – volume: 133 start-page: 1486 year: 1961 end-page: 1487 ident: bib28 article-title: Control of osmotic pressure of culture solutions with polyethylene glycol publication-title: Science – volume: 12 start-page: 231 year: 2014 end-page: 239 ident: bib61 article-title: Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves publication-title: Plant Biotechnol. J. – volume: 55 start-page: 49 year: 2002 end-page: 59 ident: bib4 article-title: The intricate pathway of starch biosynthesis and degradation in the monocellular alga publication-title: Aust. J. Chem. – volume: 10 start-page: 169 year: 2014 end-page: 183 ident: bib32 article-title: Advances in the production of high-value products by microalgae publication-title: Ind. Biotechnol. – volume: 94 start-page: 1491 year: 2014 end-page: 1499 ident: bib67 article-title: Effects of different plant hormones or PEG seed soaking on maize resistance to drought stress publication-title: Can. J. Plant Sci. – volume: 92 start-page: 881 year: 1990 end-page: 885 ident: bib47 article-title: Molecular characterization of the publication-title: Plant Physiol. – volume: 9 start-page: 1440 year: 2020 ident: bib12 article-title: Influence of polyethylene glycol on leaf anatomy, stomatal behavior, water loss, and some physiological traits of date palm plantlets grown publication-title: Plants – volume: 54 start-page: 255 year: 2006 end-page: 259 ident: bib48 article-title: Isolation and characterization of a starchless mutant of publication-title: Phycol. Res. – volume: 11 start-page: 330 year: 2018 ident: bib45 article-title: Seed-specific suppression of ADP-glucose pyrophosphorylase in publication-title: Biotechnol. Biofuels – volume: 183 start-page: 1069 year: 2001 end-page: 1077 ident: bib68 article-title: Starchless mutants of publication-title: J. Bacteriol. – volume: 31 start-page: 579 year: 2012 end-page: 587 ident: bib66 article-title: PEG and ABA trigger the burst of reactive oxygen species to increase tanshinone production in salvia miltiorrhiza hairy roots publication-title: J. Plant Growth Regul. – volume: 26 start-page: 180 year: 2017 end-page: 189 ident: bib27 article-title: Improvement of biomass and lipid yield under stress conditions by using diploid strains of publication-title: Algal Res. – volume: 37 start-page: 1669 year: 2014 end-page: 1677 ident: bib31 article-title: Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae publication-title: Bioprocess Biosyst. Eng. – volume: 69 start-page: 501 year: 1970 end-page: 513 ident: bib30 article-title: Absorption of polyethylene glycols by plants and their effects on plant growth publication-title: N. Phytol. – volume: 10 start-page: 1444 year: 2019 ident: bib64 article-title: Upregulated Lipid Biosynthesis at the expense of starch production in potato ( publication-title: Front. Plant Sci. – volume: 258 start-page: 379 year: 2021 end-page: 390 ident: bib69 article-title: Effects of simulated drought stress on carotenoid contents and expression of related genes in carrot taproots publication-title: Protoplasma – volume: 21 start-page: 277 year: 2010 end-page: 286 ident: bib53 article-title: Biodiesel from algae: challenges and prospects publication-title: Curr. Opin. Biotechnol. – volume: 329 year: 2021 ident: bib11 article-title: Insights into upstream processing of microalgae: a review publication-title: Bioresour. Technol. – volume: 11 start-page: 1229 year: 1992 end-page: 1238 ident: bib44 article-title: Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes publication-title: EMBO J. – volume: 54 start-page: 1665 year: 1965 end-page: 1669 ident: bib18 article-title: Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of publication-title: Proc. Natl. Acad. Sci. – volume: 271 start-page: 16281 year: 1996 end-page: 16287 ident: bib60 article-title: Control of starch composition and structure through substrate supply in the monocellular alga publication-title: J. Biol. Chem. – volume: 15 start-page: 124 year: 2016 end-page: 130 ident: bib56 article-title: Characterization of the growth, chlorophyll content and lipid accumulation in a marine microalgae publication-title: J. Ocean Univ. China – volume: 49 start-page: 6288 year: 2010 end-page: 6308 ident: bib26 article-title: Poly(ethylene glycol) in drug delivery: pros and cons as well as potential alternatives publication-title: Angew. Chem. Int. Ed. – start-page: 1 year: 2022 end-page: 28 ident: bib59 article-title: Production of microalgae with high lipid content and their potential as sources of nutraceuticals publication-title: Phytochem. Rev. – volume: 102 start-page: 100 year: 2009 end-page: 112 ident: bib50 article-title: Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor publication-title: Biotechnol. Bioeng. – volume: 8 year: 2018 ident: bib16 article-title: Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga publication-title: Sci. Rep. – volume: 30 start-page: 289 year: 2003 end-page: 295 ident: bib20 article-title: Constructs and methods for high-throughput gene silencing in plants publication-title: Methods – volume: 146 start-page: 173 year: 2012 end-page: 183 ident: bib65 article-title: PEG and ABA trigger methyl jasmonate accumulation to induce the MEP pathway and increase tanshinone production in publication-title: Physiol. Plant. – volume: 37 start-page: 911 year: 1959 end-page: 917 ident: bib7 article-title: A rapid method of total lipid extraction and purification publication-title: Can. J. Biochem. Physiol. – volume: 106 start-page: 10348 year: 2009 end-page: 10353 ident: bib57 article-title: Starch as a major integrator in the regulation of plant growth publication-title: Proc. Natl. Acad. Sci. – volume: 7 year: 2023 ident: bib15 article-title: Microalgae—sustainable source for alternative proteins and functional ingredients promoting gut and liver health publication-title: Glob. Chall. – volume: 22 start-page: 239 year: 2023 ident: bib40 article-title: Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae publication-title: Microb. Cell Factor. – volume: 148 start-page: 350 year: 1987 end-page: 382 ident: bib36 article-title: [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes publication-title: In – volume: 95 start-page: 716 year: 1991 end-page: 722 ident: bib21 article-title: Water relations of growing maize coleoptiles 1: comparison between mannitol and polyethylene glycol 6000 as external osmotica for adjusting turgor pressure publication-title: Plant Physiol. – volume: 57 start-page: 610 year: 2012 end-page: 616 ident: bib35 article-title: Solubilities of CO2 in Poly(ethylene glycols) from (303.15 to 333.15) K publication-title: J. Chem. Eng. Data – volume: 142 start-page: 119 year: 2017 end-page: 125 ident: bib54 article-title: Silencing publication-title: J. Am. Soc. Hortic. Sci. – reference: Xavier, J., Yadav, R.M., & Subramanyam, R. (2022). Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress (p. 2022.08.30.505872). bioRxiv. – volume: 592 start-page: 2739 year: 2018 end-page: 2759 ident: bib3 article-title: Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions publication-title: FEBS Lett. – volume: 108 start-page: 766 year: 2011 end-page: 776 ident: bib8 article-title: Microalgae—Novel highly efficient starch producers publication-title: Biotechnol. Bioeng. – volume: 96 start-page: 919 year: 2020 end-page: 928 ident: bib22 article-title: Improvement of ionizing gamma irradiation tolerance of publication-title: Int. J. Radiat. Biol. – volume: 15 start-page: 658 year: 2017 end-page: 668 ident: bib19 article-title: RNAi-mediated endogene silencing in strawberry fruit: detection of primary and secondary siRNAs by deep sequencing publication-title: Plant Biotechnol. J. – volume: 4 start-page: 1 year: 1968 end-page: 4 ident: bib1 article-title: Simple conditions for growth of unicellular blue-green algae on plates1, 2 publication-title: J. Phycol. – volume: 86 start-page: 1131 year: 1988 end-page: 1135 ident: bib37 article-title: Isolation and characterization of a starchless mutant of publication-title: Plant Physiol. – volume: 4 year: 2021 ident: bib25 article-title: Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme publication-title: Commun. Biol. – volume: 263 start-page: 633 year: 1988 end-page: 637 ident: bib43 article-title: Affinity labeling of the allosteric activator site(s) of spinach leaf ADP-glucose pyrophosphorylase publication-title: J. Biol. Chem. – volume: 46 start-page: 13487 year: 2022 end-page: 13510 ident: bib9 article-title: Co-production of bioethanol and commercially important exopolysaccharides from the marine cyanobacterium publication-title: Int. J. Energy Res. – volume: 312 year: 2020 ident: bib55 article-title: Response of Scenedesmus sp. to microwave treatment: Enhancement of lipid, exopolysaccharide and biomass production publication-title: Bioresour. Technol. – volume: 291 year: 2019 ident: bib49 article-title: Storage of starch and lipids in microalgae: biosynthesis and manipulation by nutrients publication-title: Bioresour. Technol. – volume: 720 year: 2020 ident: bib70 article-title: Using polyethylene glycol to promote publication-title: Sci. Total Environ. – volume: 12 start-page: 387 year: 2010 end-page: 391 ident: bib33 article-title: starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol publication-title: Metab. Eng. – volume: 17 start-page: 261 year: 2002 end-page: 264 ident: bib6 article-title: Low molecular weight polyethylene glycol induces chromosome aberrations in Chinese hamster cells cultured publication-title: Mutagenesis – reference: . – volume: 28 start-page: 350 year: 1956 end-page: 356 ident: bib14 article-title: Colorimetric method for determination of sugars and related substances publication-title: Anal. Chem. – volume: 110 start-page: 469 year: 2000 end-page: 476 ident: bib17 article-title: Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity publication-title: Physiol. Plant. – volume: 8 start-page: 2672 year: 2017 end-page: 2685 ident: bib41 article-title: Microalgae as healthy ingredients for functional food: a review publication-title: Food Funct. – volume: 17 start-page: 447 year: 2005 end-page: 460 ident: bib5 article-title: An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae publication-title: J. Appl. Phycol. – volume: 9 start-page: 255 year: 2016 ident: bib58 article-title: The dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth publication-title: Biotechnol. Biofuels – volume: 165 start-page: 2739 year: 2020 end-page: 2749 ident: bib39 article-title: Microalgae starch: a promising raw material for the bioethanol production publication-title: Int. J. Biol. Macromol. – volume: 21 start-page: 277 issue: 3 year: 2010 ident: 10.1016/j.jbiotec.2025.04.007_bib53 article-title: Biodiesel from algae: challenges and prospects publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2010.03.005 – volume: 94 start-page: 1491 issue: 8 year: 2014 ident: 10.1016/j.jbiotec.2025.04.007_bib67 article-title: Effects of different plant hormones or PEG seed soaking on maize resistance to drought stress publication-title: Can. J. Plant Sci. doi: 10.4141/cjps-2014-110 – ident: 10.1016/j.jbiotec.2025.04.007_bib63 doi: 10.1101/2022.08.30.505872 – volume: 271 start-page: 16281 issue: 27 year: 1996 ident: 10.1016/j.jbiotec.2025.04.007_bib60 article-title: Control of starch composition and structure through substrate supply in the monocellular alga Chlamydomonas reinhardtii publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.27.16281 – volume: 30 start-page: 289 issue: 4 year: 2003 ident: 10.1016/j.jbiotec.2025.04.007_bib20 article-title: Constructs and methods for high-throughput gene silencing in plants publication-title: Methods doi: 10.1016/S1046-2023(03)00036-7 – volume: 37 start-page: 911 issue: 8 year: 1959 ident: 10.1016/j.jbiotec.2025.04.007_bib7 article-title: A rapid method of total lipid extraction and purification publication-title: Can. J. Biochem. Physiol. doi: 10.1139/o59-099 – start-page: 2012 year: 2012 ident: 10.1016/j.jbiotec.2025.04.007_bib24 article-title: Water Stress Effects on Leaf Growth and Chlorophyll Content but Not the Grain Yield in Traditional Rice (Oryza sativa Linn.) Genotypes of Assam, India II. Protein and Proline Status in Seedlings under PEG Induced Water Stress publication-title: Am. J. Plant Sci. – volume: 57 start-page: 610 issue: 2 year: 2012 ident: 10.1016/j.jbiotec.2025.04.007_bib35 article-title: Solubilities of CO2 in Poly(ethylene glycols) from (303.15 to 333.15) K publication-title: J. Chem. Eng. Data doi: 10.1021/je201197m – volume: 40 start-page: 271 issue: 9 year: 2024 ident: 10.1016/j.jbiotec.2025.04.007_bib52 article-title: Engineered Chlorella vulgaris improves bioethanol production and promises prebiotic application publication-title: World J. Microbiol. Biotechnol. doi: 10.1007/s11274-024-04074-z – volume: 4 issue: 1 year: 2021 ident: 10.1016/j.jbiotec.2025.04.007_bib25 article-title: Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme publication-title: Commun. Biol. doi: 10.1038/s42003-021-01976-8 – volume: 17 start-page: 447 issue: 5 year: 2005 ident: 10.1016/j.jbiotec.2025.04.007_bib5 article-title: An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae publication-title: J. Appl. Phycol. doi: 10.1007/s10811-005-1641-4 – volume: 106 start-page: 10348 issue: 25 year: 2009 ident: 10.1016/j.jbiotec.2025.04.007_bib57 article-title: Starch as a major integrator in the regulation of plant growth publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.0903478106 – volume: 8 issue: 1 year: 2018 ident: 10.1016/j.jbiotec.2025.04.007_bib16 article-title: Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae publication-title: Sci. Rep. doi: 10.1038/s41598-018-30809-8 – volume: 55 start-page: 49 issue: 2 year: 2002 ident: 10.1016/j.jbiotec.2025.04.007_bib4 article-title: The intricate pathway of starch biosynthesis and degradation in the monocellular alga Chlamydomonas reinhardtii publication-title: Aust. J. Chem. doi: 10.1071/CH02052 – volume: 69 start-page: 501 issue: 2 year: 1970 ident: 10.1016/j.jbiotec.2025.04.007_bib30 article-title: Absorption of polyethylene glycols by plants and their effects on plant growth publication-title: N. Phytol. doi: 10.1111/j.1469-8137.1970.tb02446.x – volume: 8 start-page: 2672 issue: 8 year: 2017 ident: 10.1016/j.jbiotec.2025.04.007_bib41 article-title: Microalgae as healthy ingredients for functional food: a review publication-title: Food Funct. doi: 10.1039/C7FO00409E – volume: 291 year: 2019 ident: 10.1016/j.jbiotec.2025.04.007_bib49 article-title: Storage of starch and lipids in microalgae: biosynthesis and manipulation by nutrients publication-title: Bioresour. Technol. doi: 10.1016/j.biortech.2019.121894 – volume: 59 start-page: 1880 issue: 12 year: 2019 ident: 10.1016/j.jbiotec.2025.04.007_bib2 article-title: Industrial potential of carotenoid pigments from microalgae: current trends and future prospects publication-title: Crit. Rev. Food Sci. Nutr. doi: 10.1080/10408398.2018.1432561 – volume: 107 start-page: 258 issue: 2 year: 2010 ident: 10.1016/j.jbiotec.2025.04.007_bib34 article-title: Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.22807 – volume: 86 start-page: 1131 issue: 4 year: 1988 ident: 10.1016/j.jbiotec.2025.04.007_bib37 article-title: Isolation and characterization of a starchless mutant of Arabidopsis thaliana (L.) Heynh lacking ADPglucose pyrophosphorylase activity 1 publication-title: Plant Physiol. doi: 10.1104/pp.86.4.1131 – start-page: 1 year: 2022 ident: 10.1016/j.jbiotec.2025.04.007_bib59 article-title: Production of microalgae with high lipid content and their potential as sources of nutraceuticals publication-title: Phytochem. Rev. – volume: 165 start-page: 2739 year: 2020 ident: 10.1016/j.jbiotec.2025.04.007_bib39 article-title: Microalgae starch: a promising raw material for the bioethanol production publication-title: Int. J. Biol. Macromol. doi: 10.1016/j.ijbiomac.2020.10.159 – volume: 263 start-page: 633 issue: 2 year: 1988 ident: 10.1016/j.jbiotec.2025.04.007_bib43 article-title: Affinity labeling of the allosteric activator site(s) of spinach leaf ADP-glucose pyrophosphorylase publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)35399-2 – volume: 2014 year: 2014 ident: 10.1016/j.jbiotec.2025.04.007_bib38 article-title: Cloning and expression of a cytosolic HSP90 Gene in Chlorella vulgaris publication-title: BioMed. Res. Int. – volume: 22 start-page: 239 issue: 1 year: 2023 ident: 10.1016/j.jbiotec.2025.04.007_bib40 article-title: Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae publication-title: Microb. Cell Factor. doi: 10.1186/s12934-023-02244-6 – volume: 26 start-page: 180 year: 2017 ident: 10.1016/j.jbiotec.2025.04.007_bib27 article-title: Improvement of biomass and lipid yield under stress conditions by using diploid strains of Chlamydomonas reinhardtii publication-title: Algal Res. doi: 10.1016/j.algal.2017.07.027 – volume: 329 year: 2021 ident: 10.1016/j.jbiotec.2025.04.007_bib11 article-title: Insights into upstream processing of microalgae: a review publication-title: Bioresour. Technol. doi: 10.1016/j.biortech.2021.124870 – volume: 146 start-page: 173 issue: 2 year: 2012 ident: 10.1016/j.jbiotec.2025.04.007_bib65 article-title: PEG and ABA trigger methyl jasmonate accumulation to induce the MEP pathway and increase tanshinone production in Salvia miltiorrhiza hairy roots publication-title: Physiol. Plant. doi: 10.1111/j.1399-3054.2012.01603.x – volume: 28 start-page: 350 issue: 3 year: 1956 ident: 10.1016/j.jbiotec.2025.04.007_bib14 article-title: Colorimetric method for determination of sugars and related substances publication-title: Anal. Chem. doi: 10.1021/ac60111a017 – volume: 10 start-page: 169 issue: 3 year: 2014 ident: 10.1016/j.jbiotec.2025.04.007_bib32 article-title: Advances in the production of high-value products by microalgae publication-title: Ind. Biotechnol. doi: 10.1089/ind.2013.0039 – volume: 9 start-page: 255 issue: 1 year: 2016 ident: 10.1016/j.jbiotec.2025.04.007_bib58 article-title: The dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth publication-title: Biotechnol. Biofuels doi: 10.1186/s13068-016-0671-2 – volume: 720 year: 2020 ident: 10.1016/j.jbiotec.2025.04.007_bib70 article-title: Using polyethylene glycol to promote Nannochloropsis oceanica growth with 15 vol% CO2 publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2020.137598 – volume: 110 start-page: 469 issue: 4 year: 2000 ident: 10.1016/j.jbiotec.2025.04.007_bib17 article-title: Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity publication-title: Physiol. Plant. doi: 10.1111/j.1399-3054.2000.1100407.x – volume: 11 start-page: 1229 issue: 4 year: 1992 ident: 10.1016/j.jbiotec.2025.04.007_bib44 article-title: Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes publication-title: EMBO J. doi: 10.1002/j.1460-2075.1992.tb05167.x – volume: 102 start-page: 100 issue: 1 year: 2009 ident: 10.1016/j.jbiotec.2025.04.007_bib50 article-title: Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.22033 – volume: 96 start-page: 919 issue: 7 year: 2020 ident: 10.1016/j.jbiotec.2025.04.007_bib22 article-title: Improvement of ionizing gamma irradiation tolerance of Chlorella vulgaris by pretreatment with polyethylene glycol publication-title: Int. J. Radiat. Biol. doi: 10.1080/09553002.2020.1741717 – volume: 11 start-page: 330 issue: 1 year: 2018 ident: 10.1016/j.jbiotec.2025.04.007_bib45 article-title: Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight publication-title: Biotechnol. Biofuels doi: 10.1186/s13068-018-1334-2 – volume: 10 start-page: 1444 year: 2019 ident: 10.1016/j.jbiotec.2025.04.007_bib64 article-title: Upregulated Lipid Biosynthesis at the expense of starch production in potato (Solanum tuberosum) vegetative tissues via simultaneous downregulation of adp-glucose pyrophosphorylase and sugar dependent1 expressions publication-title: Front. Plant Sci. doi: 10.3389/fpls.2019.01444 – volume: 183 start-page: 1069 issue: 3 year: 2001 ident: 10.1016/j.jbiotec.2025.04.007_bib68 article-title: Starchless mutants of Chlamydomonas reinhardtii lack the small subunit of a heterotetrameric ADP-glucose pyrophosphorylase publication-title: J. Bacteriol. doi: 10.1128/JB.183.3.1069-1077.2001 – volume: 108 start-page: 766 issue: 4 year: 2011 ident: 10.1016/j.jbiotec.2025.04.007_bib8 article-title: Microalgae—Novel highly efficient starch producers publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.23016 – volume: 258 start-page: 379 issue: 2 year: 2021 ident: 10.1016/j.jbiotec.2025.04.007_bib69 article-title: Effects of simulated drought stress on carotenoid contents and expression of related genes in carrot taproots publication-title: Protoplasma doi: 10.1007/s00709-020-01570-5 – volume: 95 start-page: 716 issue: 3 year: 1991 ident: 10.1016/j.jbiotec.2025.04.007_bib21 article-title: Water relations of growing maize coleoptiles 1: comparison between mannitol and polyethylene glycol 6000 as external osmotica for adjusting turgor pressure publication-title: Plant Physiol. doi: 10.1104/pp.95.3.716 – volume: 4 start-page: 1 issue: 1 year: 1968 ident: 10.1016/j.jbiotec.2025.04.007_bib1 article-title: Simple conditions for growth of unicellular blue-green algae on plates1, 2 publication-title: J. Phycol. doi: 10.1111/j.1529-8817.1968.tb04667.x – volume: 54 start-page: 1665 issue: 6 year: 1965 ident: 10.1016/j.jbiotec.2025.04.007_bib18 article-title: Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.54.6.1665 – volume: 31 start-page: 579 issue: 4 year: 2012 ident: 10.1016/j.jbiotec.2025.04.007_bib66 article-title: PEG and ABA trigger the burst of reactive oxygen species to increase tanshinone production in salvia miltiorrhiza hairy roots publication-title: J. Plant Growth Regul. doi: 10.1007/s00344-012-9268-6 – volume: 31 start-page: 426 issue: 3 year: 1959 ident: 10.1016/j.jbiotec.2025.04.007_bib42 article-title: Use of dinitrosalicylic acid reagent for determination of reducing sugar publication-title: Anal. Chem. doi: 10.1021/ac60147a030 – volume: 13 start-page: 173 issue: 3 year: 2003 ident: 10.1016/j.jbiotec.2025.04.007_bib10 article-title: Influence of water stress on biomass production by isolates of an ericoid mycorrhizal endophyte of Woollsia pungens and Epacris microphylla (Ericaceae) publication-title: Mycorrhiza doi: 10.1007/s00572-003-0228-7 – volume: 12 start-page: 13 issue: 1 year: 1990 ident: 10.1016/j.jbiotec.2025.04.007_bib13 article-title: Isolation of plant DNA from fresh tissue publication-title: Focus – volume: 312 year: 2020 ident: 10.1016/j.jbiotec.2025.04.007_bib55 article-title: Response of Scenedesmus sp. to microwave treatment: Enhancement of lipid, exopolysaccharide and biomass production publication-title: Bioresour. Technol. doi: 10.1016/j.biortech.2020.123562 – volume: 142 start-page: 119 issue: 2 year: 2017 ident: 10.1016/j.jbiotec.2025.04.007_bib54 article-title: Silencing GhAGPL1 reduces the quality and quantity of corms and cormels in gladiolus publication-title: J. Am. Soc. Hortic. Sci. doi: 10.21273/JASHS03944-16 – volume: 148 start-page: 350 year: 1987 ident: 10.1016/j.jbiotec.2025.04.007_bib36 article-title: [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes – volume: 9 start-page: 1440 issue: 11 year: 2020 ident: 10.1016/j.jbiotec.2025.04.007_bib12 article-title: Influence of polyethylene glycol on leaf anatomy, stomatal behavior, water loss, and some physiological traits of date palm plantlets grown In Vitro and Ex Vitro publication-title: Plants doi: 10.3390/plants9111440 – volume: 93 start-page: 785 issue: 2 year: 1990 ident: 10.1016/j.jbiotec.2025.04.007_bib46 article-title: The subunit structure of potato tuber ADPglucose pyrophosphorylase 1 publication-title: Plant Physiol. doi: 10.1104/pp.93.2.785 – volume: 7 issue: 5 year: 2023 ident: 10.1016/j.jbiotec.2025.04.007_bib15 article-title: Microalgae—sustainable source for alternative proteins and functional ingredients promoting gut and liver health publication-title: Glob. Chall. – volume: 15 start-page: 658 issue: 5 year: 2017 ident: 10.1016/j.jbiotec.2025.04.007_bib19 article-title: RNAi-mediated endogene silencing in strawberry fruit: detection of primary and secondary siRNAs by deep sequencing publication-title: Plant Biotechnol. J. doi: 10.1111/pbi.12664 – volume: 27 start-page: 581 issue: 6 year: 2001 ident: 10.1016/j.jbiotec.2025.04.007_bib62 article-title: Construct design for efficient, effective and high-throughput gene silencing in plants publication-title: Plant J.: Cell Mol. Biol. doi: 10.1046/j.1365-313X.2001.01105.x – volume: 12 start-page: 387 issue: 4 year: 2010 ident: 10.1016/j.jbiotec.2025.04.007_bib33 article-title: Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol publication-title: Metab. Eng. doi: 10.1016/j.ymben.2010.02.002 – volume: 15 start-page: 124 issue: 1 year: 2016 ident: 10.1016/j.jbiotec.2025.04.007_bib56 article-title: Characterization of the growth, chlorophyll content and lipid accumulation in a marine microalgae Dunaliella tertiolecta under different nitrogen to phosphorus ratios publication-title: J. Ocean Univ. China doi: 10.1007/s11802-016-2797-z – volume: 37 start-page: 1669 issue: 8 year: 2014 ident: 10.1016/j.jbiotec.2025.04.007_bib31 article-title: Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae Nannochloropsis oculata publication-title: Bioprocess Biosyst. Eng. doi: 10.1007/s00449-014-1139-z – volume: 12 start-page: 76 issue: 1 year: 2021 ident: 10.1016/j.jbiotec.2025.04.007_bib51 article-title: Microalgal-based feed: promising alternative feedstocks for livestock and poultry production publication-title: J. Anim. Sci. Biotechnol. doi: 10.1186/s40104-021-00593-z – volume: 16 start-page: 378 year: 2018 ident: 10.1016/j.jbiotec.2025.04.007_bib23 article-title: Transformation of Dunaliella parva with PSY gene: carotenoids show enhanced antioxidant activity under polyethylene glycol and calcium treatments publication-title: Biocatal. Agric. Biotechnol. doi: 10.1016/j.bcab.2018.09.011 – volume: 46 start-page: 13487 issue: 10 year: 2022 ident: 10.1016/j.jbiotec.2025.04.007_bib9 article-title: Co-production of bioethanol and commercially important exopolysaccharides from the marine cyanobacterium Synechococcus elongatus BDU 10144 in a novel low-cost seawater-fertilizer-based medium publication-title: Int. J. Energy Res. doi: 10.1002/er.8069 – volume: 133 start-page: 1486 issue: 3463 year: 1961 ident: 10.1016/j.jbiotec.2025.04.007_bib28 article-title: Control of osmotic pressure of culture solutions with polyethylene glycol publication-title: Science doi: 10.1126/science.133.3463.1486 – volume: 12 start-page: 231 issue: 2 year: 2014 ident: 10.1016/j.jbiotec.2025.04.007_bib61 article-title: Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves publication-title: Plant Biotechnol. J. doi: 10.1111/pbi.12131 – volume: 20 start-page: 336 issue: 5 year: 2022 ident: 10.1016/j.jbiotec.2025.04.007_bib29 article-title: Exopolysaccharides from microalgae and cyanobacteria: diversity of strains, production strategies, and applications publication-title: Mar. Drugs doi: 10.3390/md20050336 – volume: 592 start-page: 2739 issue: 16 year: 2018 ident: 10.1016/j.jbiotec.2025.04.007_bib3 article-title: Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions publication-title: FEBS Lett. doi: 10.1002/1873-3468.13198 – volume: 54 start-page: 255 issue: 4 year: 2006 ident: 10.1016/j.jbiotec.2025.04.007_bib48 article-title: Isolation and characterization of a starchless mutant of Chlorella pyrenoidosa STL-PI with a high growth rate, and high protein and polyunsaturated fatty acid content publication-title: Phycol. Res. doi: 10.1111/j.1440-1835.2006.00416.x – volume: 17 start-page: 261 issue: 3 year: 2002 ident: 10.1016/j.jbiotec.2025.04.007_bib6 article-title: Low molecular weight polyethylene glycol induces chromosome aberrations in Chinese hamster cells cultured in vitro publication-title: Mutagenesis doi: 10.1093/mutage/17.3.261 – volume: 49 start-page: 6288 issue: 36 year: 2010 ident: 10.1016/j.jbiotec.2025.04.007_bib26 article-title: Poly(ethylene glycol) in drug delivery: pros and cons as well as potential alternatives publication-title: Angew. Chem. Int. Ed. doi: 10.1002/anie.200902672 – volume: 92 start-page: 881 issue: 4 year: 1990 ident: 10.1016/j.jbiotec.2025.04.007_bib47 article-title: Molecular characterization of the Brittle-2 gene effect on maize endosperm ADPglucose pyrophosphorylase subunits publication-title: Plant Physiol. doi: 10.1104/pp.92.4.881
SSID	ssj0004951
Score	2.476847
Snippet	Microalgae with rapid growth rate, ability to be cultivated in non-agricultural land, and producing numerous bioactive compounds have attracted attention for...
SourceID	proquest pubmed crossref elsevier
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	81
SubjectTerms	acetates AGPase biobased products biorefining biosynthesis biotechnology carbohydrate content carbon Carotenoids Chlorella vulgaris Chlorella vulgaris - drug effects Chlorella vulgaris - enzymology Chlorella vulgaris - genetics Chlorella vulgaris - growth & development Chlorella vulgaris - metabolism coproducts culture media exopolysaccharides fuel production Gene Silencing genes genetically modified organisms glucose-1-phosphate adenylyltransferase Glucose-1-Phosphate Adenylyltransferase - genetics Glucose-1-Phosphate Adenylyltransferase - metabolism lipid content liquid state fermentation metabolites microalgae PEG 6000 phosphates polyethylene glycol Polyethylene Glycols - chemistry Polyethylene Glycols - pharmacology RNA Interference RNA interference, starch biosynthesis starch Starch - biosynthesis Starch - metabolism
Title	Enhanced accumulation of important bioproducts in Chlorella vulgaris through AGPase gene silencing coupled with polyethylene glycol treatment
URI	https://dx.doi.org/10.1016/j.jbiotec.2025.04.007 https://www.ncbi.nlm.nih.gov/pubmed/40222633 https://www.proquest.com/docview/3189915859 https://www.proquest.com/docview/3206211973
Volume	403
WOSCitedRecordID	wos001471943500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1873-4863 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0004951 issn: 0168-1656 databaseCode: AIEXJ dateStart: 20210110 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3JbtswECWcpIf2UHSvuwQsUPRiyLVFSaSORuB0S9wAdgrfBIqiKhmKpHqD8xG99ns7FLUYcN2kh14EgxAl2u-ZM8PZEHobmGZgchkarm2bhmWGgeGSsG8ERBIeUuL4RbT7tzM6GrHp1L1otX5VuTDrhKYp22zc_L9CDWMAtkqd_Qe464fCAHwG0OEKsMP1VsAP00h79bkQq6uyO1dRGeKq0LXTZcePs1xXei2iYU8iMNpVFFRnvVKZHfGibt8z-HABYk71WZadRawSlHSS7ipPqrj1PEuuJcCdqHu-J9fArCZ8fY_uCwtY7pzpj7l2Po1huwyiWlxM-Ub7pGQyh8WOI152_C7ChqJsEelJQZw3Z7bnPNZxCufzou3Xl-3TDdOuI2GrDZlRYlis3ATl7tiOCNCnEbPuTH-VrnpqV9dIb2Re5ecfffVOL8_OvMlwOnmX_zBUNzLltS9bsxygI5PaLuyWR4NPw-nnJuPWLRp71itpcsPe__HN-7SefVZNod1MHqD7JTR4oOn0ELVk-gjd2ypW-Rj9rIiFt4mFsxDXxMJbxMJximti4YpYuCQW1sTCili4JhYuiYUVsfA2sbAmFq6J9QRdng4nJx-NspmHIUBMLI3QZ7wnOEiUwOecuNKnliOcvvAF8x3bt8zAESYXPlgEVDLQIykXzFX1MpkbEJs8RYdplsrnCIfEFf2QBqAz9C3KJFfOdAcsFUZFnwunjazqx_ZEWeleNVxJvCqkceaVGHkKI69neYBRG3Xrabku9XLTBFYh6ZX6qtZDPeDiTVPfVMh7sJ8rJx1PZbZaeCBjwWQDI979yz1mz1GVGSlpo2eaNvWKLXWC4xDy4hZveInuNv-5V-hwOV_J1-iOWC_jxfwYHdApOy6Z_xvKsuFW
linkProvider	Elsevier
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Enhanced+accumulation+of+important+bioproducts+in+Chlorella+vulgaris+through+AGPase+gene+silencing+coupled+with+polyethylene+glycol+treatment&rft.jtitle=Journal+of+biotechnology&rft.au=Saha%2C+Sumedha&rft.au=Xaxa%2C+Delrin+Shaina&rft.au=Ghosh%2C+Sudip+K&rft.au=Maiti%2C+Mrinal+K&rft.date=2025-07-01&rft.issn=1873-4863&rft.eissn=1873-4863&rft_id=info:doi/10.1016%2Fj.jbiotec.2025.04.007&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0168-1656&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0168-1656&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0168-1656&client=summon