Biological reference materials for extracellular vesicle studies

Extracellular vesicles (EVs) mediate normal physiological homeostasis and pathological processes by facilitating intercellular communication. Research of EVs in basic science and clinical settings requires both methodological standardization and development of reference materials (RM). Here, we show...

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Veröffentlicht in:European journal of pharmaceutical sciences Jg. 98; S. 4 - 16
Hauptverfasser: Valkonen, S., van der Pol, E., Böing, A., Yuana, Y., Yliperttula, M., Nieuwland, R., Laitinen, S., Siljander, P.R.M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 15.02.2017
Schlagworte:
Characterization
Erythrocytes - cytology
Extracellular vesicles
Extracellular Vesicles - ultrastructure
Flow Cytometry
Humans
Microscopy, Electron, Transmission
Nanoerythrosome
Nanostructures - chemistry
Nanostructures - standards
Nanostructures - ultrastructure
Proteolipids - chemistry
Proteolipids - standards
Quantification
Reference material
Reference Standards
Standardization
Characterization
Extracellular vesicles
Reference material
Nanoerythrosome
Quantification
Standardization
ISSN:0928-0987, 1879-0720, 1879-0720
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Zusammenfassung:Extracellular vesicles (EVs) mediate normal physiological homeostasis and pathological processes by facilitating intercellular communication. Research of EVs in basic science and clinical settings requires both methodological standardization and development of reference materials (RM). Here, we show insights and results of biological RM development for EV studies. We used a three-step approach to find and develop a biological RM. First, a literature search was done to find candidates for biological RMs. Second, a questionnaire was sent to EV researchers querying the preferences for RM and their use. Third, a biological RM was selected, developed, characterized, and evaluated. The responses to the survey demonstrated a clear and recognized need for RM optimized for the calibration of EV measurements. Based on the literature, naturally occurring and produced biological RM, such as virus particles and liposomes, were proposed as RM. However, none of these candidate RMs have properties completely matching those of EVs, such as size and refractive index distribution. Therefore, we evaluated the use of nanoerythrosomes (NanoE), vesicles produced from erythrocytes, as a potential biological RM. The strength of NanoE is their resemblance to EVs. Compared to the erythrocyte-derived EVs (eryEVs), NanoE have similar morphology, a similar refractive index (1.37), larger diameter (70% of the NanoE are over 200nm), and increased positive staining for CD235a and lipids (Di-8-ANEPPS) (58% and 67% in NanoE vs. 21% and 45% in eryEVs, respectively). Altogether, our results highlight the general need to develop and validate new RM with similar physical and biochemical properties as EVs to standardize EV measurements between instruments and laboratories. [Display omitted]
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0928-0987
1879-0720
1879-0720
DOI:10.1016/j.ejps.2016.09.008