Structural characterization and anticancer properties of a novel mushroom-derived rhamnogalactan in a triple-negative breast cancer model

Mushroom-derived polysaccharides are known for their diverse biological activities, including immunomodulatory and anticancer properties. Breast cancer, the leading cause of cancer-related death among women worldwide, lacks targeted molecular therapies, underscoring the need for novel therapeutic ag...

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Bibliographic Details
Published in:Carbohydrate polymers Vol. 364; p. 123735
Main Authors: de Lara, Eliane Leal, Zavadinack, Matheus, da Silva Milhorini, Shayane, Wurzer, Karin Maciel, Klassen, Giseli, Cordeiro, Lucimara M.C., Iacomini, Marcello
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15.09.2025
Subjects:
additive effect
Agaricales - chemistry
Anticancer effect
Antineoplastic Agents - chemistry
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
breast neoplasms
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
cell viability
death
drug therapy
drugs
Female
Galactans - chemistry
Galactans - isolation & purification
Galactans - pharmacology
galactose
Heteropolysaccharides
Humans
Lactarius
Lactarius quieticolor
molecular weight
mushrooms
paclitaxel
Paclitaxel - pharmacology
polysaccharides
Rhamnogalactan
rhamnose
Triple Negative Breast Neoplasms - drug therapy
Triple Negative Breast Neoplasms - pathology
Triple-negative breast cancer
Heteropolysaccharides
Anticancer effect
Lactarius quieticolor
Rhamnogalactan
Triple-negative breast cancer
ISSN:0144-8617, 1879-1344, 1879-1344
Online Access:Get full text
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Summary:Mushroom-derived polysaccharides are known for their diverse biological activities, including immunomodulatory and anticancer properties. Breast cancer, the leading cause of cancer-related death among women worldwide, lacks targeted molecular therapies, underscoring the need for novel therapeutic agents. In this study, we extracted, purified, and characterized a novel heteropolysaccharide (HTP) - a rhamnogalactan - composed of galactose (72.6 %) and rhamnose (27.4 %) from the edible mushroom Lactarius quieticolor. Structural analysis revealed that HTP consists of a (1 → 6)-linked α-d-galactopyranose backbone, heavily substituted at O-2 by non-reducing rhamnose units, with a weight-average molar mass of 2.41 × 104 g.mol−1. In vitro assays using a triple-negative breast cancer (TNBC) cell model - the most aggressive subtype - showed a significant reduction in cell viability (50 % at 1200 μg·mL−1). Furthermore, an additive effect was observed when co-administered with the chemotherapy drug paclitaxel, reducing the cell viability to 42 %. These findings suggest potential therapeutic applications of the purified mushroom rhamnogalactan reported in this study against TNBC. [Display omitted]
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ISSN:0144-8617
1879-1344
1879-1344
DOI:10.1016/j.carbpol.2025.123735