Standardization of Laboratory Methods for the PERCH Study
The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from child...
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| Published in: | Clinical infectious diseases Vol. 64; no. suppl_3; p. S245 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
15.06.2017
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| ISSN: | 1537-6591, 1537-6591 |
| Online Access: | Get more information |
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| Abstract | The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1-59 months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory. |
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| AbstractList | The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1-59 months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory. The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1-59 months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory.The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1-59 months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory. |
| Author | Hammitt, Laura L Endtz, Hubert P Panchalingam, Sandra Madhi, Shabir A Mwarumba, Salim Morpeth, Susan C Mitchell, Joanne L O'Brien, Katherine L Nyongesa, Sammy Ahmed, Dilruba Sawatwong, Pongpun Gitahi, Caroline Karani, Angela Morailane, Palesa Maiga, Abdoul Aziz Murdoch, David R Baggett, Henry C Baillie, Vicky L Levine, Orin S Howie, Stephen R C Karron, Ruth A Bhat, Niranjan Brooks, W Abdullah Anderson, Trevor P Dione, Michel Thea, Donald M Toure, Aliou Kotloff, Karen L Adrian, Peter V Scott, J Anthony G Mugo, Daisy Knoll, Maria Deloria Mwaba, John Rahman, Mustafizur Driscoll, Amanda J Alam, Muntasir Kwenda, Geoffrey Mwansa, James Whistler, Toni Feikin, Daniel R McClellan, Jessica Antonio, Martin Tamboura, Boubou |
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Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia – sequence: 7 givenname: W Abdullah surname: Brooks fullname: Brooks, W Abdullah organization: Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland – sequence: 8 givenname: Daniel R surname: Feikin fullname: Feikin, Daniel R organization: Division of Viral Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia – sequence: 9 givenname: Laura L surname: Hammitt fullname: Hammitt, Laura L organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 10 givenname: Stephen R C surname: Howie fullname: Howie, Stephen R C organization: Centre for International Health, University of Otago, Dunedin, New Zealand – sequence: 11 givenname: Maria Deloria surname: Knoll fullname: Knoll, Maria Deloria organization: International Vaccine Access Center, and – sequence: 12 givenname: Karen L surname: Kotloff fullname: Kotloff, Karen L organization: Division of Infectious Disease and Tropical Pediatrics, Department of Pediatrics, Center for Vaccine Development, Institute of Global Health, University of Maryland School of Medicine, Baltimore – sequence: 13 givenname: Shabir A surname: Madhi fullname: Madhi, Shabir A organization: Department of Science and Technology/National Research Foundation, Vaccine Preventable Diseases Unit, University of the Witwatersrand, Johannesburg, South Africa – sequence: 14 givenname: J Anthony G surname: Scott fullname: Scott, J Anthony G organization: Department of Infectious Disease Epidemiology, London School of Hygiene & Tropical Medicine, United Kingdom – sequence: 15 givenname: Donald M surname: Thea fullname: Thea, Donald M organization: Center for Global Health and Development, Boston University School of Public Health, Massachusetts – sequence: 16 givenname: Peter V surname: Adrian fullname: Adrian, Peter V organization: Department of Science and Technology/National Research Foundation, Vaccine Preventable Diseases Unit, University of the Witwatersrand, Johannesburg, South Africa – sequence: 17 givenname: Dilruba surname: Ahmed fullname: Ahmed, Dilruba organization: InternationalCentre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka and Matlab – sequence: 18 givenname: Muntasir surname: Alam fullname: Alam, Muntasir organization: International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka – sequence: 19 givenname: Trevor P surname: Anderson fullname: Anderson, Trevor P organization: Microbiology Department, Canterbury Health Laboratories, Christchurch, New Zealand – sequence: 20 givenname: Martin surname: Antonio fullname: Antonio, Martin organization: Microbiology and Infection Unit, Warwick Medical School, University of Warwick, Coventry, United Kingdom – sequence: 21 givenname: Vicky L surname: Baillie fullname: Baillie, Vicky L organization: Department of Science and Technology/National Research Foundation, Vaccine Preventable Diseases Unit, University of the Witwatersrand, Johannesburg, South Africa – sequence: 22 givenname: Michel surname: Dione fullname: Dione, Michel organization: International Livestock Research Institute, Kampala, Uganda – sequence: 23 givenname: Hubert P surname: Endtz fullname: Endtz, Hubert P organization: Fondation Mérieux, Lyon, France – sequence: 24 givenname: Caroline surname: Gitahi fullname: Gitahi, Caroline organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 25 givenname: Angela surname: Karani fullname: Karani, Angela organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 26 givenname: Geoffrey surname: Kwenda fullname: Kwenda, Geoffrey organization: Zambia Center for Applied Health Research and Development, Lusaka – sequence: 27 givenname: Abdoul Aziz surname: Maiga fullname: Maiga, Abdoul Aziz organization: Centre pour le Développement des Vaccins (CVD-Mali), Bamako – sequence: 28 givenname: Jessica surname: McClellan fullname: McClellan, Jessica organization: Cummings School of Medicine, University of Calgary, Canada – sequence: 29 givenname: Joanne L surname: Mitchell fullname: Mitchell, Joanne L organization: Microbiology Department, Canterbury Health Laboratories, Christchurch, New Zealand – sequence: 30 givenname: Palesa surname: Morailane fullname: Morailane, Palesa organization: Department of Science and Technology/National Research Foundation, Vaccine Preventable Diseases Unit, University of the Witwatersrand, Johannesburg, South Africa – sequence: 31 givenname: Daisy surname: Mugo fullname: Mugo, Daisy organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 32 givenname: John surname: Mwaba fullname: Mwaba, John organization: Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia – sequence: 33 givenname: James surname: Mwansa fullname: Mwansa, James organization: Cummings School of Medicine, University of Calgary, Canada – sequence: 34 givenname: Salim surname: Mwarumba fullname: Mwarumba, Salim organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 35 givenname: Sammy surname: Nyongesa fullname: Nyongesa, Sammy organization: Kenya Medical Research Institute-Wellcome Trust Research Programme, Kilifi – sequence: 36 givenname: Sandra surname: Panchalingam fullname: Panchalingam, Sandra organization: Department of Medicine, Center for Vaccine Development, Institute of Global Health, University of Maryland School of Medicine, Baltimore; and – sequence: 37 givenname: Mustafizur surname: Rahman fullname: Rahman, Mustafizur organization: International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka – sequence: 38 givenname: Pongpun surname: Sawatwong fullname: Sawatwong, Pongpun organization: Global Disease Detection Center, Thailand Ministry of Public Health-US Centers for Disease Control and Prevention Collaboration, Nonthaburi – sequence: 39 givenname: Boubou surname: Tamboura fullname: Tamboura, Boubou organization: Centre pour le Développement des Vaccins (CVD-Mali), Bamako – sequence: 40 givenname: Aliou surname: Toure fullname: Toure, Aliou organization: Centre pour le Développement des Vaccins (CVD-Mali), Bamako – sequence: 41 givenname: Toni surname: Whistler fullname: Whistler, Toni organization: Division of Global Health Protection, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia – sequence: 42 givenname: Katherine L surname: O'Brien fullname: O'Brien, Katherine L organization: International Vaccine Access Center, and – sequence: 43 givenname: David R surname: Murdoch fullname: Murdoch, David R organization: Department of Pathology, University of Otago, Christchurch, New Zealand |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28575358$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Algorithms Child, Preschool Clinical Laboratory Techniques - standards Data Accuracy Female HIV Infections Humans Infant Male Pneumonia - diagnosis Pneumonia - etiology Pneumonia, Bacterial - diagnosis Pneumonia, Viral - diagnosis Quality Control Reference Standards Respiratory Tract Infections - etiology Specimen Handling - standards |
| Title | Standardization of Laboratory Methods for the PERCH Study |
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