Quantification of tumor-derived cell free DNA(cfDNA) by digital PCR (DigPCR) in cerebrospinal fluid of patients with BRAFV600 mutated malignancies

Tumor-derived cell free DNA (cfDNA) can be detected in plasma. We hypothesized that mutated BRAF V600 cfDNA could be quantified in the cerebrospinal fluid (CSF) of patients with central nervous system (CNS) metastases. We collected CSF from patients with BRAF V600E or K-mutated melanoma (N=8) or BRA...

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Bibliographic Details
Published in:	Oncotarget Vol. 7; no. 51; p. 85430
Main Authors:	Momtaz, Parisa, Pentsova, Elena, Abdel-Wahab, Omar, Diamond, Eli, Hyman, David, Merghoub, Taha, You, Daoqi, Gasmi, Billel, Viale, Agnes, Chapman, Paul B
Format:	Journal Article
Language:	English
Published:	United States 20.12.2016
Subjects:	Adult Aged Biomarkers, Tumor - cerebrospinal fluid Biomarkers, Tumor - genetics Central Nervous System Neoplasms - cerebrospinal fluid Central Nervous System Neoplasms - enzymology Central Nervous System Neoplasms - genetics Central Nervous System Neoplasms - secondary Circulating Tumor DNA - cerebrospinal fluid Circulating Tumor DNA - genetics DNA Mutational Analysis - methods Erdheim-Chester Disease - cerebrospinal fluid Erdheim-Chester Disease - enzymology Erdheim-Chester Disease - genetics Erdheim-Chester Disease - pathology Female Humans Magnetic Resonance Imaging Male Melanoma - cerebrospinal fluid Melanoma - enzymology Melanoma - genetics Melanoma - secondary Middle Aged Mutation Polymerase Chain Reaction - methods Predictive Value of Tests Proto-Oncogene Proteins B-raf - cerebrospinal fluid Proto-Oncogene Proteins B-raf - genetics Skin Neoplasms - cerebrospinal fluid Skin Neoplasms - enzymology Skin Neoplasms - genetics Skin Neoplasms - pathology Spinal Puncture erdheim-chester disease digital PCR BRAF mutated melanoma cell free DNA cerebrospinal fluid
ISSN:	1949-2553, 1949-2553
Online Access:	Get more information
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