The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State

Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculia...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Cancers Ročník 13; číslo 21; s. 5478
Hlavní autoři:	Spera, Iolanda, Sánchez-Rodríguez, Ricardo, Favia, Maria, Menga, Alessio, Venegas, Francisca C., Angioni, Roberta, Munari, Fabio, Lanza, Martina, Campanella, Annalisa, Pierri, Ciro L., Canton, Marcella, Castegna, Alessandra
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Basel MDPI AG 31.10.2021 MDPI
Témata:	Angiogenesis Bone marrow Breeding Cancer Cell activation Cell culture Cloning Disease Gene expression Immune system Innate immunity Investigations Macrophages Metabolism Metabolites Respiration Tumors United States > US
ISSN:	2072-6694, 2072-6694
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages.
AbstractList	Simple SummaryEvidence of the role of macrophages in promoting cancer progression has prompted scientists to investigate innate immune cell function in order to identify targetable checkpoint for reverting the protumoral functions of macrophages. Primary cultures isolated from mice necessary to investigate the mechanisms mediating immune cell activation require expensive and time-consuming breeding and housing of mice strains. We obtained an in-house generated immortalized macrophage cell line from BMDMs. In the present study, we characterize this cell line both from a functional and metabolic point of view, comparing the different parameters to those obtained from the primary counterpart. Our results indicate that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features to primary cells polarized in the same way, validating their use for in vitro studies relevant to the understanding and targeting of immune cell functions within tumors.AbstractMacrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages. Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages. Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages.Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages.
Author	Sánchez-Rodríguez, Ricardo Campanella, Annalisa Spera, Iolanda Venegas, Francisca C. Pierri, Ciro L. Castegna, Alessandra Angioni, Roberta Munari, Fabio Lanza, Martina Menga, Alessio Canton, Marcella Favia, Maria
AuthorAffiliation	4 Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, 10126 Turin, Italy; alessio.menga@unito.it 1 Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, 70125 Bari, Italy; iolandaspera@yahoo.it (I.S.); mariafavia@hotmail.com (M.F.); martinaa.lanzaa@gmail.com (M.L.); annalisa.campanella@uniba.it (A.C.); ciro.pierri@uniba.it (C.L.P.) 2 Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; ricardo.sanchezrodriguez@unipd.it (R.S.-R.); francisca-venegas@phd.unipd.it (F.C.V.); fabio.munari@unipd.it (F.M.) 3 Fondazione Istituto di Ricerca Pediatrica Città della Speranza—IRP, 35129 Padova, Italy; roberta.angioni@unipd.it
AuthorAffiliation_xml	– name: 4 Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, 10126 Turin, Italy; alessio.menga@unito.it – name: 2 Department of Biomedical Sciences, University of Padova, 35131 Padova, Italy; ricardo.sanchezrodriguez@unipd.it (R.S.-R.); francisca-venegas@phd.unipd.it (F.C.V.); fabio.munari@unipd.it (F.M.) – name: 1 Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, 70125 Bari, Italy; iolandaspera@yahoo.it (I.S.); mariafavia@hotmail.com (M.F.); martinaa.lanzaa@gmail.com (M.L.); annalisa.campanella@uniba.it (A.C.); ciro.pierri@uniba.it (C.L.P.) – name: 3 Fondazione Istituto di Ricerca Pediatrica Città della Speranza—IRP, 35129 Padova, Italy; roberta.angioni@unipd.it
Author_xml	– sequence: 1 givenname: Iolanda surname: Spera fullname: Spera, Iolanda – sequence: 2 givenname: Ricardo surname: Sánchez-Rodríguez fullname: Sánchez-Rodríguez, Ricardo – sequence: 3 givenname: Maria orcidid: 0000-0001-6039-9365 surname: Favia fullname: Favia, Maria – sequence: 4 givenname: Alessio orcidid: 0000-0002-2827-5298 surname: Menga fullname: Menga, Alessio – sequence: 5 givenname: Francisca C. surname: Venegas fullname: Venegas, Francisca C. – sequence: 6 givenname: Roberta surname: Angioni fullname: Angioni, Roberta – sequence: 7 givenname: Fabio surname: Munari fullname: Munari, Fabio – sequence: 8 givenname: Martina surname: Lanza fullname: Lanza, Martina – sequence: 9 givenname: Annalisa surname: Campanella fullname: Campanella, Annalisa – sequence: 10 givenname: Ciro L. orcidid: 0000-0003-1816-548X surname: Pierri fullname: Pierri, Ciro L. – sequence: 11 givenname: Marcella surname: Canton fullname: Canton, Marcella – sequence: 12 givenname: Alessandra surname: Castegna fullname: Castegna, Alessandra
BookMark	eNp1UU1v1DAQjVARLaVnrpa4cAn1ZxJfkGBLoWgjVqKco1ln0nWVtYPtIG3_Bn8YL1shWAlfxvK89-b5zfPixHmHRfGS0TdCaHppwBkMkQnOlKybJ8UZpzUvq0rLk7_up8VFjPc0HyFYXdXPilMh65pVkp0VP283SD7z8ma79SHBaB-wJ-0crEPSggl-2sAdkgWOI1nuH69snEbYRbLaoPNpN1kDIwGXWZhg7UdryDVCmgNG4geyCnYLYUfet1dtJNaRPNAG0rIDh5OVHyHYB0jWO_I1QcIXxdMBxogXj_W8-Hb94XbxqVx--XizeLcsjdBNKgdj-lrI9cAbRQVI5AoaULpfS4NYGVNp7FH1oh_qHpkGzitFUXIjpO5pL86LtwfdaV5vsTfoUoCxmw6OOw-2-7fj7Ka78z-6RjVcNToLvH4UCP77jDF1WxtNjgoc-jl2XOlaaqVUlaGvjqD3fg4uf2-PqiijWtKMujygcvAxBhz-mGG02--8O9p5ZqgjhrHpd5bZsR3_y_sF0La00Q
CitedBy_id	crossref_primary_10_1016_j_isci_2023_106938 crossref_primary_10_1038_s41418_022_01076_y crossref_primary_10_3389_fimmu_2022_923727 crossref_primary_10_1002_art_42697 crossref_primary_10_1039_D3SC06431J crossref_primary_10_1080_21505594_2025_2508813 crossref_primary_10_26508_lsa_202402760 crossref_primary_10_3389_fimmu_2024_1379853 crossref_primary_10_3389_fcimb_2024_1457323
Cites_doi	10.1038/ni.1937 10.1038/sj.onc.1202786 10.1038/ncomms15556 10.1038/s41392-021-00506-6 10.3791/53424 10.1002/1873-3468.12744 10.3389/fimmu.2019.01462 10.1016/j.febslet.2014.11.015 10.1016/j.immuni.2015.02.005 10.1038/nature11986 10.15252/emmm.201911210 10.1074/jbc.M113.522037 10.1016/j.celrep.2017.07.054 10.1016/j.pharmthera.2020.107521 10.1016/j.bbagrm.2014.07.013 10.1038/s41467-020-14433-7 10.1186/1743-8977-5-7 10.1016/j.kint.2017.07.026 10.1016/0008-8749(88)90236-5 10.2967/jnumed.112.119099 10.1016/j.addr.2015.11.009 10.1007/s00726-019-02719-0 10.1128/jvi.65.2.842-851.1991 10.1093/jnci/81.19.1492 10.1111/febs.14295 10.1073/pnas.1917663117 10.1172/JCI59643 10.1016/j.bbabio.2015.04.009 10.3389/fonc.2018.00049 10.1155/2017/9624760 10.1016/B978-0-12-417028-5.00006-5 10.1038/ni.2956 10.1089/ars.2016.6715 10.1016/j.cmet.2006.05.011 10.1016/0165-5728(90)90073-V 10.1016/j.ccr.2004.11.022 10.4049/jimmunol.177.10.7303 10.1038/318667a0 10.1073/pnas.0810199105 10.1038/cmi.2016.35 10.1104/pp.113.218081 10.1016/j.immuni.2013.04.005 10.1016/j.bbrc.2013.09.037 10.1038/ncomms5436
ContentType	Journal Article
Copyright	2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2021 by the authors. 2021
Copyright_xml	– notice: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2021 by the authors. 2021
DBID	AAYXX CITATION 3V. 7T5 7TO 7XB 8FE 8FH 8FK 8G5 ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ GUQSH H94 HCIFZ LK8 M2O M7P MBDVC PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM
DOI	10.3390/cancers13215478
DatabaseName	CrossRef ProQuest Central (Corporate) Immunology Abstracts Oncogenes and Growth Factors Abstracts ProQuest Central (purchase pre-March 2016) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) (purchase pre-March 2016) Research Library (Alumni Edition) ProQuest Central ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea ProQuest Central Student Research Library Prep AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Biological Science Collection Research Library Biological Science Database Research Library (Corporate) ProQuest Central Premium ProQuest One Academic (New) ProQuest Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles)
DatabaseTitle	CrossRef Publicly Available Content Database Research Library Prep ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College Research Library (Alumni Edition) ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences Natural Science Collection ProQuest Central Korea Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Research Library ProQuest Central (New) ProQuest Biological Science Collection ProQuest Central Basic ProQuest One Academic Eastern Edition Biological Science Database ProQuest SciTech Collection ProQuest One Academic UKI Edition Immunology Abstracts ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	Publicly Available Content Database CrossRef MEDLINE - Academic
Database_xml	– sequence: 1 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Medicine
EISSN	2072-6694
ExternalDocumentID	PMC8582589 10_3390_cancers13215478
GeographicLocations	United States--US
GeographicLocations_xml	– name: United States--US
GroupedDBID	--- 53G 5VS 8FE 8FH 8G5 AADQD AAFWJ AAYXX ABDBF ABUWG ACUHS ADBBV AFFHD AFKRA AFZYC ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ CCPQU CITATION DIK DWQXO E3Z EBD ESX GNUQQ GUQSH GX1 HCIFZ HYE IAO IHR ITC KQ8 LK8 M2O M48 M7P MODMG M~E OK1 P6G PGMZT PHGZM PHGZT PIMPY PQGLB PQQKQ PROAC RPM TUS 3V. 7T5 7TO 7XB 8FK H94 MBDVC PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 5PM
ID	FETCH-LOGICAL-c398t-fccd734bf28503a4e25a8a59db4cee6cc69ede5d3df7de19a22650e42c349d0d3
IEDL.DBID	M7P
ISICitedReferencesCount	8
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000719018700001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2072-6694
IngestDate	Tue Nov 04 02:02:27 EST 2025 Fri Sep 05 14:46:53 EDT 2025 Fri Jul 25 12:04:14 EDT 2025 Sat Nov 29 07:16:06 EST 2025 Tue Nov 18 22:13:47 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	21
Language	English
License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c398t-fccd734bf28503a4e25a8a59db4cee6cc69ede5d3df7de19a22650e42c349d0d3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Iolanda Spera and Ricardo Sánchez-Rodríguez contributed equally to this work.
ORCID	0000-0002-2827-5298 0000-0001-6039-9365 0000-0003-1816-548X
OpenAccessLink	https://www.proquest.com/docview/2596010940?pq-origsite=%requestingapplication%
PMID	34771641
PQID	2596010940
PQPubID	2032421
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_8582589 proquest_miscellaneous_2597495556 proquest_journals_2596010940 crossref_primary_10_3390_cancers13215478 crossref_citationtrail_10_3390_cancers13215478
PublicationCentury	2000
PublicationDate	20211031
PublicationDateYYYYMMDD	2021-10-31
PublicationDate_xml	– month: 10 year: 2021 text: 20211031 day: 31
PublicationDecade	2020
PublicationPlace	Basel
PublicationPlace_xml	– name: Basel
PublicationTitle	Cancers
PublicationYear	2021
Publisher	MDPI AG MDPI
Publisher_xml	– name: MDPI AG – name: MDPI
References	Palmieri (ref_19) 2014; 588 Sica (ref_29) 2012; 122 Latour (ref_42) 2020; 52 Jha (ref_25) 2015; 42 Palmieri (ref_28) 2017; 20 Tavakoli (ref_6) 2013; 54 Zallot (ref_21) 2013; 162 Ryan (ref_26) 2017; 591 Selak (ref_37) 2005; 7 Vats (ref_7) 2006; 4 Poh (ref_34) 2018; 8 Freemerman (ref_5) 2014; 289 Yang (ref_4) 2014; 5 Infantino (ref_40) 2013; 440 ref_18 Ratsimandresy (ref_17) 2017; 8 Huang (ref_8) 2014; 15 Viola (ref_27) 2019; 10 Nielsen (ref_33) 2017; 2017 Palmieri (ref_41) 2020; 11 Pearce (ref_3) 2013; 38 Roberson (ref_14) 1988; 116 Leek (ref_30) 1996; 56 Mazzone (ref_36) 2018; 285 Wise (ref_45) 2008; 105 Ratsimandresy (ref_16) 2017; 14 Tannahill (ref_9) 2013; 496 Zhou (ref_15) 2008; 5 Palmieri (ref_20) 2017; 26 Blasi (ref_12) 1990; 27 Infantino (ref_39) 2014; 1839 Wagner (ref_46) 1993; 4 Palmieri (ref_38) 2015; 1847 Cox (ref_13) 1989; 81 Blasi (ref_11) 1985; 318 Lee (ref_43) 1999; 18 Komohara (ref_32) 2016; 99 Tang (ref_24) 2018; 93 Martinez (ref_2) 2006; 177 Castegna (ref_35) 2020; 210 Biswas (ref_31) 2010; 11 Koskinen (ref_44) 1991; 65 Menga (ref_22) 2020; 12 Locati (ref_1) 2013; 120 Duan (ref_10) 2021; 6 Tang (ref_23) 2020; 117
References_xml	– volume: 11 start-page: 889 year: 2010 ident: ref_31 article-title: Macrophage Plasticity and Interaction with Lymphocyte Subsets: Cancer as a Paradigm publication-title: Nat. Immunol. doi: 10.1038/ni.1937 – volume: 4 start-page: 879 year: 1993 ident: ref_46 article-title: C-Myc Induces the Expression and Activity Ornithine Decarboxylase publication-title: Cell Growth Differ. – volume: 18 start-page: 2997 year: 1999 ident: ref_43 article-title: The V-Myc Oncogene publication-title: Oncogene doi: 10.1038/sj.onc.1202786 – volume: 8 start-page: 15556 year: 2017 ident: ref_17 article-title: The PYRIN Domain-Only Protein POP2 Inhibits Inflammasome Priming and Activation publication-title: Nat. Commun. doi: 10.1038/ncomms15556 – volume: 6 start-page: 127 year: 2021 ident: ref_10 article-title: Targeting Macrophages in Cancer Immunotherapy publication-title: Signal. Transduct. Target. Ther. doi: 10.1038/s41392-021-00506-6 – ident: ref_18 doi: 10.3791/53424 – volume: 591 start-page: 2992 year: 2017 ident: ref_26 article-title: Krebs Cycle Rewired for Macrophage and Dendritic Cell Effector Functions publication-title: FEBS Lett. doi: 10.1002/1873-3468.12744 – volume: 56 start-page: 4625 year: 1996 ident: ref_30 article-title: Association of Macrophage Infiltration with Angiogenesis and Prognosis in Invasive Breast Carcinoma publication-title: Cancer Res. – volume: 10 start-page: 1462 year: 2019 ident: ref_27 article-title: The Metabolic Signature of Macrophage Responses publication-title: Front. Immunol. doi: 10.3389/fimmu.2019.01462 – volume: 588 start-page: 4807 year: 2014 ident: ref_19 article-title: Glutamine Synthetase Desensitizes Differentiated Adipocytes to Proinflammatory Stimuli by Raising Intracellular Glutamine Levels publication-title: FEBS Lett. doi: 10.1016/j.febslet.2014.11.015 – volume: 42 start-page: 419 year: 2015 ident: ref_25 article-title: Network Integration of Parallel Metabolic and Transcriptional Data Reveals Metabolic Modules That Regulate Macrophage Polarization publication-title: Immunity doi: 10.1016/j.immuni.2015.02.005 – volume: 496 start-page: 238 year: 2013 ident: ref_9 article-title: Succinate Is an Inflammatory Signal That Induces IL-1β through HIF-1α publication-title: Nature doi: 10.1038/nature11986 – volume: 12 start-page: e11210 year: 2020 ident: ref_22 article-title: Glufosinate Constrains Synchronous and Metachronous Metastasis by Promoting Anti-Tumor Macrophages publication-title: EMBO Mol. Med. doi: 10.15252/emmm.201911210 – volume: 289 start-page: 7884 year: 2014 ident: ref_5 article-title: Metabolic Reprogramming of Macrophages: Glucose Transporter 1 (GLUT1)-Mediated Glucose Metabolism Drives a Proinflammatory Phenotype publication-title: J. Biol. Chem. doi: 10.1074/jbc.M113.522037 – volume: 20 start-page: 1654 year: 2017 ident: ref_28 article-title: Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis publication-title: Cell Rep. doi: 10.1016/j.celrep.2017.07.054 – volume: 210 start-page: 107521 year: 2020 ident: ref_35 article-title: Pharmacological Targets of Metabolism in Disease: Opportunities from Macrophages publication-title: Pharmacol. Ther. doi: 10.1016/j.pharmthera.2020.107521 – volume: 1839 start-page: 1217 year: 2014 ident: ref_39 article-title: A Key Role of the Mitochondrial Citrate Carrier (SLC25A1) in TNFα- and IFNγ-Triggered Inflammation publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbagrm.2014.07.013 – volume: 11 start-page: 698 year: 2020 ident: ref_41 article-title: Nitric Oxide Orchestrates Metabolic Rewiring in M1 Macrophages by Targeting Aconitase 2 and Pyruvate Dehydrogenase publication-title: Nat. Commun. doi: 10.1038/s41467-020-14433-7 – volume: 5 start-page: 7 year: 2008 ident: ref_15 article-title: Characterization of Immortalized MARCO and SR-AI/II-Deficient Murine Alveolar Macrophage Cell Lines publication-title: Part. Fibre Toxicol. doi: 10.1186/1743-8977-5-7 – volume: 93 start-page: 173 year: 2018 ident: ref_24 article-title: The Proto-Oncogene Tyrosine Protein Kinase Src Is Essential for Macrophage-Myofibroblast Transition during Renal Scarring publication-title: Kidney Int. doi: 10.1016/j.kint.2017.07.026 – volume: 116 start-page: 341 year: 1988 ident: ref_14 article-title: Immortalization of Cloned Mouse Splenic Macrophages with a Retrovirus Containing the V-Raf/Mil and v-Myc Oncogenes publication-title: Cell Immunol. doi: 10.1016/0008-8749(88)90236-5 – volume: 54 start-page: 1661 year: 2013 ident: ref_6 article-title: Bioenergetic Profiles Diverge during Macrophage Polarization: Implications for the Interpretation of 18F-FDG PET Imaging of Atherosclerosis publication-title: J. Nucl. Med. doi: 10.2967/jnumed.112.119099 – volume: 99 start-page: 180 year: 2016 ident: ref_32 article-title: Tumor-Associated Macrophages: Potential Therapeutic Targets for Anti-Cancer Therapy publication-title: Adv. Drug Deliv. Rev. doi: 10.1016/j.addr.2015.11.009 – volume: 52 start-page: 151 year: 2020 ident: ref_42 article-title: The Role of Polyamines in the Regulation of Macrophage Polarization and Function publication-title: Amino Acids doi: 10.1007/s00726-019-02719-0 – volume: 65 start-page: 842 year: 1991 ident: ref_44 article-title: Nuclear Colocalization of Cellular and Viral Myc Proteins with HSP70 in Myc-Overexpressing Cells publication-title: J. Virol. doi: 10.1128/jvi.65.2.842-851.1991 – volume: 81 start-page: 1492 year: 1989 ident: ref_13 article-title: Heterogeneity of Hematopoietic Cells Immortalized by V-Myc/v-Raf Recombinant Retrovirus Infection of Bone Marrow or Fetal Liver publication-title: J. Natl. Cancer Inst. doi: 10.1093/jnci/81.19.1492 – volume: 285 start-page: 700 year: 2018 ident: ref_36 article-title: Metabolism and TAM Functions—It Takes Two to Tango publication-title: FEBS J. doi: 10.1111/febs.14295 – volume: 117 start-page: 20741 year: 2020 ident: ref_23 article-title: Neural Transcription Factor Pou4f1 Promotes Renal Fibrosis via Macrophage-Myofibroblast Transition publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1917663117 – volume: 122 start-page: 787 year: 2012 ident: ref_29 article-title: Macrophage Plasticity and Polarization: In Vivo Veritas publication-title: J. Clin. Investig. doi: 10.1172/JCI59643 – volume: 1847 start-page: 729 year: 2015 ident: ref_38 article-title: Acetylation of Human Mitochondrial Citrate Carrier Modulates Mitochondrial Citrate/Malate Exchange Activity to Sustain NADPH Production during Macrophage Activation publication-title: Biochim. Biophys. Acta-Bioenerg. doi: 10.1016/j.bbabio.2015.04.009 – volume: 8 start-page: 49 year: 2018 ident: ref_34 article-title: Targeting Macrophages in Cancer: From Bench to Bedside publication-title: Front. Oncol. doi: 10.3389/fonc.2018.00049 – volume: 2017 start-page: 9624760 year: 2017 ident: ref_33 article-title: Macrophages as Key Drivers of Cancer Progression and Metastasis publication-title: Mediat. Inflamm. doi: 10.1155/2017/9624760 – volume: 120 start-page: 163 year: 2013 ident: ref_1 article-title: Macrophage Activation and Polarization as an Adaptive Component of Innate Immunity publication-title: Adv. Immunol. doi: 10.1016/B978-0-12-417028-5.00006-5 – volume: 15 start-page: 846 year: 2014 ident: ref_8 article-title: Cell-Intrinsic Lysosomal Lipolysis Is Essential for Alternative Activation of Macrophages publication-title: Nat. Immunol. doi: 10.1038/ni.2956 – volume: 26 start-page: 351 year: 2017 ident: ref_20 article-title: Blockade of Glutamine Synthetase Enhances Inflammatory Response in Microglial Cells publication-title: Antioxid. Redox Signal. doi: 10.1089/ars.2016.6715 – volume: 4 start-page: 13 year: 2006 ident: ref_7 article-title: Oxidative Metabolism and PGC-1beta Attenuate Macrophage-Mediated Inflammation publication-title: Cell Metab. doi: 10.1016/j.cmet.2006.05.011 – volume: 27 start-page: 229 year: 1990 ident: ref_12 article-title: Immortalization of Murine Microglial Cells by a V-Raf/v-Myc Carrying Retrovirus publication-title: J. Neuroimmunol. doi: 10.1016/0165-5728(90)90073-V – volume: 7 start-page: 77 year: 2005 ident: ref_37 article-title: Succinate Links TCA Cycle Dysfunction to Oncogenesis by Inhibiting HIF-Alpha Prolyl Hydroxylase publication-title: Cancer Cell doi: 10.1016/j.ccr.2004.11.022 – volume: 177 start-page: 7303 year: 2006 ident: ref_2 article-title: Transcriptional Profiling of the Human Monocyte-to-Macrophage Differentiation and Polarization: New Molecules and Patterns of Gene Expression publication-title: J. Immunol. doi: 10.4049/jimmunol.177.10.7303 – volume: 318 start-page: 667 year: 1985 ident: ref_11 article-title: Selective Immortalization of Murine Macrophages from Fresh Bone Marrow by a Raf/Myc Recombinant Murine Retrovirus publication-title: Nature doi: 10.1038/318667a0 – volume: 105 start-page: 18782 year: 2008 ident: ref_45 article-title: Myc Regulates a Transcriptional Program That Stimulates Mitochondrial Glutaminolysis and Leads to Glutamine Addiction publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0810199105 – volume: 14 start-page: 127 year: 2017 ident: ref_16 article-title: The AIM2 Inflammasome Is a Central Regulator of Intestinal Homeostasis through the IL-18/IL-22/STAT3 Pathway publication-title: Cell Mol. Immunol. doi: 10.1038/cmi.2016.35 – volume: 162 start-page: 581 year: 2013 ident: ref_21 article-title: Identification of Mitochondrial Coenzyme a Transporters from Maize and Arabidopsis publication-title: Plant. Physiol. doi: 10.1104/pp.113.218081 – volume: 38 start-page: 633 year: 2013 ident: ref_3 article-title: Metabolic Pathways in Immune Cell Activation and Quiescence publication-title: Immunity doi: 10.1016/j.immuni.2013.04.005 – volume: 440 start-page: 105 year: 2013 ident: ref_40 article-title: ATP-Citrate Lyase Is Essential for Macrophage Inflammatory Response publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2013.09.037 – volume: 5 start-page: 4436 year: 2014 ident: ref_4 article-title: PKM2 Regulates the Warburg Effect and Promotes HMGB1 Release in Sepsis publication-title: Nat. Commun. doi: 10.1038/ncomms5436
SSID	ssj0000331767
Score	2.3004413
Snippet	Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition,... Simple SummaryEvidence of the role of macrophages in promoting cancer progression has prompted scientists to investigate innate immune cell function in order...
SourceID	pubmedcentral proquest crossref
SourceType	Open Access Repository Aggregation Database Enrichment Source Index Database
StartPage	5478
SubjectTerms	Angiogenesis Bone marrow Breeding Cancer Cell activation Cell culture Cloning Disease Gene expression Immune system Innate immunity Investigations Macrophages Metabolism Metabolites Respiration Tumors
Title	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
URI	https://www.proquest.com/docview/2596010940 https://www.proquest.com/docview/2597495556 https://pubmed.ncbi.nlm.nih.gov/PMC8582589
Volume	13
WOSCitedRecordID	wos000719018700001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2072-6694 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331767 issn: 2072-6694 databaseCode: M~E dateStart: 20090101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2072-6694 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331767 issn: 2072-6694 databaseCode: M7P dateStart: 20090101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2072-6694 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331767 issn: 2072-6694 databaseCode: BENPR dateStart: 20090101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2072-6694 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331767 issn: 2072-6694 databaseCode: PIMPY dateStart: 20090101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Research Library customDbUrl: eissn: 2072-6694 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331767 issn: 2072-6694 databaseCode: M2O dateStart: 20090101 isFulltext: true titleUrlDefault: https://search.proquest.com/pqrl providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZoixCX8qxYKCsjceASmthxYp8QfYmiZolQQctp5dgTbaQoWTbZSuVv8IfxJNktiwQXjnE8cpIZe755ZIaQ1waVIDDrMZkLL7QavMyhBE_LjOVKhiboCml_vYwnEzmdqnRwuDVDWuX6TOwOalsb9JEfOZiOtoMK_XeL7x52jcLo6tBCY4fsYZUE3qXupRsfi8-ddozivqIPd9b9kcFPuWycCRZgIattZXSLMLfzI39TOOcP_vdRH5L9AWrS971sPCJ3oHpM7iVDMP0J-elEhH5k3oUjQAxe_ABLE_S-A000tvaau8OGnkBZ0kscPC2aRalvGprOoarbmwUymOrKUUHrhKksDEVIuXImPK1zmvaVLOhxcpo0tKjoFUYlaBL0NIymaFcPP4LSDvY-JV_Oz65OPnhDjwbPcCVbLzfGxjzMciaFz3UITGiphbJZ6NRvZEykwIKw3OaxhUBpB_eEDyEzPFTWt_yA7FZ1Bc8INYH2YxtlkGsbMuDaBlJHigHwwGgmRuTtmlkzMxQwxz4a5cwZMsjd2R_cHZE3G4JF_8Z_n3q4ZuVs2MTN7JaPI_Jqc9ttP4yp6ArqVTcndjamENGIxFtSs1kSC3hv36mKeVfIWwpnn0v1_N-LvyD3GSbSdArzkOy2yxW8JHfNdVs0yzHZiadyTPaOzybpZ3eVsE_jbhe4sfQiSb_9Ao6GFaY
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3NjtMwELaWBQEX_hGFBYwEEpewiR0n9gEh2LLask3VQ0F7C649USNVSWlSUHkN3oNnxJOkXYoEtz1wje04sT_PfGOPZwh5blAJArMek5nwQqvBmzqW4Gk5ZZmSoQmaQNqfhvFoJM_O1HiP_NzchUG3yo1MbAS1LQ3ukR86mo62gwr9N4svHmaNwtPVTQqNFhansP7mTLbq9aDv5vcFY8fvJ0cnXpdVwDNcydrLjLExD6cZk8LnOgQmtNRC2WnoFEZkTKTAgrDcZrGFQGlHUIQPITM8VNa33L33ErnsaASTjavgeLun43OnjaO4jSDEufIPDU7dsnImX4CBs3aV3zmj3fXH_E3BHd_834bmFrnRUWn6tsX-bbIHxR1yNemcBe6SH24J0A_MG7gPRBsj_w6WJni6ADTRmLps5oQpPYL5nA7xYT-vFnO9ruh4BkVZrxcIYKoL1wpqt1jmuaFImVdLqGiZ0XEbqYO-S_pJRfOCTvDUhSZB24bRMe4bdBddaUPr75GPFzIk98l-URbwgFATaD-20RQybUMGXNtA6kgxAB4YzUSPvNqAIzVdgHbMEzJPnaGGaEr_QFOPvNw2WLR__PeqBxvopJ2QqtJz3PTIs22xEy94ZqQLKFdNndjZ0EJEPRLvoHTbJQYo3y0p8lkTqFwKyYRUD__d-VNy7WSSDNPhYHT6iFxn6DTUkIMDsl8vV_CYXDFf67xaPmlWGyWfLxrDvwDU83Ch
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V3NjtMwELaWBa24LP-iywJGAolLaGLHiX1ACLZUlG2qHBa0t6xrT9RIVVqaFlReg7fh6fAkaZciwW0PXGM7Tuxv_jzjGUKeGxSCwKzHZC680Grwxk5L8LQcs1zJ0AR1Iu3Pw3g0kufnKt0jPzd3YTCscsMTa0ZtZwbPyLtOTUfbQYV-N2_DItJe_838i4cVpNDTuimn0UDkFNbfnPlWvR703F6_YKz__uzkg9dWGPAMV3Lp5cbYmIfjnEnhcx0CE1pqoew4dMIjMiZSYEFYbvPYQqC0U1aEDyEzPFTWt9y99xq5HmPS8jpsMN2e7_jcSeYobrIJca78rsFtXFTO_AswidauILzUbndjM38Tdv1b__My3SaHrYpN3zY0cYfsQXmXHCRtEME98sORBv3IvIH7QLQ9iu9gaYJeB6CJxpJmE8dk6QlMp3SID3tFNZ_qdUXTCZSz5XqOwKa6dKNg6YhoWhiKqvRqARWd5TRtMnjQd0kvqWhR0jP0xtAkaMYwmuJ5QnsBltbq_n3y6UqW5AHZL2clPCTUBNqPbTSGXNuQAdc2kDpSDIAHRjPRIa82QMlMm7gd64dMM2fAIbKyP5DVIS-3A-bNH_-96_EGRlnLvKrsEkMd8mzb7NgO-pJ0CbNV3Sd2trUQUYfEO4jdTomJy3dbymJSJzCXQjIh1dG_J39KDhx0s-FgdPqI3GQYS1TrDMdkf7lYwWNyw3xdFtXiSU14lFxcNYR_AQH8eV4
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+J2-Immortalized+Murine+Macrophage+Cell+Line+Displays+Phenotypical+and+Metabolic+Features+of+Primary+BMDMs+in+Their+M1+and+M2+Polarization+State&rft.jtitle=Cancers&rft.au=Spera%2C+Iolanda&rft.au=S%C3%A1nchez-Rodr%C3%ADguez%2C+Ricardo&rft.au=Favia%2C+Maria&rft.au=Menga%2C+Alessio&rft.date=2021-10-31&rft.pub=MDPI+AG&rft.eissn=2072-6694&rft.volume=13&rft.issue=21&rft.spage=5478&rft_id=info:doi/10.3390%2Fcancers13215478&rft.externalDBID=HAS_PDF_LINK
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2072-6694&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2072-6694&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2072-6694&client=summon