Inflammatory bowel disease is associated with increased mucosal levels of bactericidal/permeability-increasing protein

Clinical sepsis seldom accompanies inflammatory bowel disease. The aim of this study was to measure colonic mucosal levels of the neutrophil product bactericidal/permeability-increasing protein (BPI), which kills gram-negative bacteria in addition to inactivating endotoxin. Enzyme-linked immunosorbe...

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Vydané v:Gastroenterology (New York, N.Y. 1943) Ročník 110; číslo 3; s. 733
Hlavní autori: Monajemi, H, Meenan, J, Lamping, R, Obradov, D O, Radema, S A, Trown, P W, Tytgat, G N, Van Deventer, S J
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.03.1996
Predmet:
Adolescent
Adult
Animals
Antimicrobial Cationic Peptides
Biopsy
Blood Proteins - metabolism
Blotting, Western
Colitis, Ulcerative - metabolism
Colon - metabolism
Colon - pathology
Crohn Disease - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Inflammatory Bowel Diseases - metabolism
Inflammatory Bowel Diseases - pathology
Intestinal Mucosa - metabolism
Intestinal Mucosa - pathology
Male
Membrane Proteins
Middle Aged
Neutrophils - pathology
ISSN:0016-5085
On-line prístup:Zistit podrobnosti o prístupe
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Popis
Shrnutí:Clinical sepsis seldom accompanies inflammatory bowel disease. The aim of this study was to measure colonic mucosal levels of the neutrophil product bactericidal/permeability-increasing protein (BPI), which kills gram-negative bacteria in addition to inactivating endotoxin. Enzyme-linked immunosorbent assay and immunohistochemistry for BPI were performed on homogenates and tissue secretions of biopsy specimens from patients with ulcerative colitis (n=11) and Crohn's disease (n=5) and from normal controls (n=5). Mucosal neutrophil content (144 +/- 23 vs. 35 +/- 9 neutrophils/mg protein; P<0.007) and BPI content (2.07 +/- 0.75 vs. 0.12 +/- 0.02 ng/mg protein; P<0.002) were greater in the colitis groups and correlated closely (r=0.68; P<0.001). This relationship held for both ulcerative colitis (P<0.002) and Crohn's disease (P<0.01) with a trend towards greater levels in Crohn's disease. There was a trend towards higher BPI levels with an increasing endoscopic inflammation score (grade I, 1.32 +/- 0.6 ng/mg protein; grade II, 2.82 +/- 1.4 ng/mg protein). Immunohistochemistry and the biopsy culture showed BPI to be both intracellular and extracellular, to be present in the crypt lumen, and to be released into incubating medium. Mucosal levels of BPI are increased in colitis. Such localization may ameliorate mucosal responses to gram-negative bacteria and their products.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0016-5085
DOI:10.1053/gast.1996.v110.pm8608882