Improved Nanopore full-length cDNA sequencing by PCR-suppression

Full-length transcript sequencing remains a main goal of RNA sequencing. However, even the application of long-read sequencing technologies such as Oxford Nanopore Technologies still fail to yield full-length transcript sequencing for a significant portion of sequenced reads. Since these technologie...

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Vydáno v:	Frontiers in genetics Ročník 13; s. 1031355
Hlavní autoři:	Bayega, Anthony, Oikonomopoulos, Spyros, Wang, Yu Chang, Ragoussis, Jiannis
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland Frontiers Media S.A 17.10.2022
Témata:	cDNA Genetics long-read sequencing nanopore panhandle PCR suppression effect RNAseq cDNA nanopore RNAseq long-read sequencing PCR suppression effect panhandle
ISSN:	1664-8021, 1664-8021
On-line přístup:	Získat plný text
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