Immunization of mice with plasmid DNA expressing the measles virus nucleoprotein gene

The measles virus (MV) nucleocapsid (N) protein gene has been inserted into a plasmid vector so as to place the gene under the control of the strong constitutive human cytomegalovirus major immediate early promoter. On intramuscular injection of pMV64 DNA into C3H/He mice, seroconversion with increa...

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Veröffentlicht in:Viral immunology Jg. 9; H. 2; S. 65
Hauptverfasser: Fooks, A R, Jeevarajah, D, Warnes, A, Wilkinson, G W, Clegg, J C
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.01.1996
Schlagworte:
Animals
Antibodies, Viral - blood
Cell Line
DNA, Viral - immunology
Gene Expression
Humans
Immunization
Immunoglobulin G - blood
Measles - prevention & control
Measles Vaccine
Measles virus - genetics
Mice
Mice, Inbred C3H
Nucleoproteins - genetics
Plasmids - genetics
Vaccines, DNA
Viral Proteins - genetics
ISSN:0882-8245
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Zusammenfassung:The measles virus (MV) nucleocapsid (N) protein gene has been inserted into a plasmid vector so as to place the gene under the control of the strong constitutive human cytomegalovirus major immediate early promoter. On intramuscular injection of pMV64 DNA into C3H/He mice, seroconversion with increasing titers of N-specific serum IgG antibodies was observed over a period of 3 months. However, when 3-week-old mice were immunized by intramuscular injection of pMV64 in a two-dose schedule, and challenged intracranially with a rodent-adapted measles virus strain (CAM/RB) at 5 weeks of age, no significant protective response was seen. The lack of effective protection evoked by DNA immunization in this model, where MV challenge must take place before 8 weeks of age, may be due to inefficient induction of cell-mediated immunity resulting from expression in muscle tissue, compounded by a relatively slow rise in immune response compared with that seen with the recombinant adenovirus.
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ISSN:0882-8245
DOI:10.1089/vim.1996.9.65