Getting the most from PSI–BLAST

Most biologists now conduct sequence searches as a matter of course. But how do we know that a relationship predicted by a homology search is a true, rather than false, hit with the same score? Many biologists design their own experiments with exquisite care yet still assume that results from progra...

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Veröffentlicht in:Trends in biochemical sciences (Amsterdam. Regular ed.) Jg. 27; H. 3; S. 161 - 164
Hauptverfasser: Jones, David T, Swindells, Mark B
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier Ltd 01.03.2002
Schlagworte:
Algorithms
Amino Acid Sequence
Animals
Databases, Factual
DNA - chemistry
Gapped BLAST
Humans
Molecular Sequence Data
protein database
protein sequence
Proteins - chemistry
PSI-BLAST
Sequence Alignment
Sequence Homology, Amino Acid
Software
protein database
Gapped BLAST
Structural Biology
Biochemistry
PSI-BLAST
Bioinformatics
protein sequence
ISSN:0968-0004, 1362-4326
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Zusammenfassung:Most biologists now conduct sequence searches as a matter of course. But how do we know that a relationship predicted by a homology search is a true, rather than false, hit with the same score? Many biologists design their own experiments with exquisite care yet still assume that results from programs with more than 20 adjustable parameters are 100% reliable. This article explains some of the key steps in getting the most from PSI–Blast, one of the most popular and powerful homology search programs currently available.
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ISSN:0968-0004
1362-4326
DOI:10.1016/S0968-0004(01)02039-4