Occurrence and antibiotic resistance of Vibrio parahaemolyticus isolated from the Tunisian coastal seawater

Vibrio parahaemolyticus is a gram-negative bacterium ubiquitous in seawater or estuarine water throughout the world. It is a major cause of seafood gastroenteritis complications. In this study, the presence of V. parahaemolyticus was investigated in 66 seawater samples collected during 2018 from 15...

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Veröffentlicht in:Journal of water and health Jg. 20; H. 2; S. 369 - 384
Hauptverfasser: Zaafrane, Sami, Maatouk, Kaouthar, Alibi, Sana, Ben Mansour, Hedi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London IWA Publishing 01.02.2022
Schlagworte:
Amikacin
Antibiotic resistance
Antibiotics
Bacteria
Brackishwater environment
Cefotaxime
Chemical analysis
Chloramphenicol
Chloromycetin
Colistin
Colonies
Disease resistance
Drug resistance
Estuaries
Gastroenteritis
Genes
Gram-negative bacteria
Identification
Laboratories
Nitrofurantoin
Nucleotide sequence
PCR
Penicillin
Polymerase chain reaction
Salinity
Seafood
Seafoods
Seawater
Selective media
Sulfamethoxazole
Target detection
ToxR gene
Trimethoprim
Vibrio parahaemolyticus
Virulence
virulence gene
Water analysis
France
Spain
ISSN:1477-8920, 1996-7829
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Zusammenfassung:Vibrio parahaemolyticus is a gram-negative bacterium ubiquitous in seawater or estuarine water throughout the world. It is a major cause of seafood gastroenteritis complications. In this study, the presence of V. parahaemolyticus was investigated in 66 seawater samples collected during 2018 from 15 stations spread along the Tunisian coast using selective media including CHROMagar Vibrio media. The results show that only eight samples contained V. parahaemolyticus. However, while Vibrio alginolyticus was detected in all samples; both Vibrio cholerae and Vibrio vulnificus were not found. Nine of the presumed V. parahaemolyticus colonies were purified on tryptic soy agar from eight positive samples then identified by the API 20E biochemical test and confirmed by the presence of a specific target toxR gene. The detection of virulence genes, thermostable direct haemolysin (tdh) and thermostable-related haemolysin (trh), by the polymerase chain reaction (PCR) showed the presence of only two trh-positive isolates. The assessment of antibiotic susceptibility of the V. parahaemolyticus isolated revealed a complete resistance to colistin, amikacin, penicillin and cefotaxime and a total sensitivity to chloramphenicol, nitrofurantoin and sulfamethoxazole-trimethoprim with a multiple antibiotic resistance index (MAR) ranging from 0.4 to 0.5.
Bibliographie:ObjectType-Article-1
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ISSN:1477-8920
1996-7829
DOI:10.2166/wh.2022.243