Changes in human hepatic metabolism in steatosis and cirrhosis

To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. We have carried out pilot study using H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic...

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Vydané v:World journal of gastroenterology : WJG Ročník 23; číslo 15; s. 2685
Hlavní autori: Schofield, Zoe, Reed, Michelle Ac, Newsome, Philip N, Adams, David H, Günther, Ulrich L, Lalor, Patricia F
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 21.04.2017
Predmet:
Adult
Aged
Fatty Liver - metabolism
Female
Humans
Liver - metabolism
Liver Cirrhosis - metabolism
Magnetic Resonance Spectroscopy
Male
Middle Aged
Human
Metabolomics
Alcohol
Nuclear magnetic resonance
Liver
Steatosis
ISSN:2219-2840, 2219-2840
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Shrnutí:To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. We have carried out pilot study using H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2219-2840
2219-2840
DOI:10.3748/wjg.v23.i15.2685