Purification, characterization and cytotoxicity assessment of Ageritin: The first ribotoxin from the basidiomycete mushroom Agrocybe aegerita
Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosyn...
Gespeichert in:
| Veröffentlicht in: | Biochimica et biophysica acta. General subjects Jg. 1861; H. 5; S. 1113 - 1121 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
Netherlands
Elsevier B.V
01.05.2017
|
| Schlagworte: | |
| ISSN: | 0304-4165, 1872-8006 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pest resistant transgenic plants.
The ribotoxin Ageritin has been isolated for the first time from the Basidiomycetes class. The enzyme, characterized by means of its amino acid composition, N-terminal sequence and a circular dichroism, structurally differs from Ascomycota ribotoxin prototype, although it was able, as α-sarcin, to release a specific α-fragment. However, it does not display aspecific ribonucleolytic activity. Ageritin exerts cytotoxicity and cell death promoting effects towards CNS model cell lines (SK-N-BE(2)-C, U-251 and C6), as vinblastine, a plant alkaloid used in cancer therapy. Moreover, our results indicate that Ageritin initially activates caspase-8, whereas caspase-9 cleavage was not detected, demonstrating the involvement of an extrinsic apoptotic pathway.
Our findings show that Ageritin is the earliest diverging member of the Ascomycota ribotoxin family, suggesting that ribotoxins are more widely distributed among fungi than previously believed.
Ageritin, structurally different from the widely known Ascomycota ribotoxins, with promising anti-cancer properties vs. aggressive brain tumours, has been found from the basidiomycete fungus Agrocybe aegerita. Finally, this finding highlights that the ribotoxin family has divergent members in Basidiomycota phylum, whose structural and functional characterization can give new information on ribotoxin or ribonuclease superfamilies.
[Display omitted]
•Ageritin is a ribotoxin purified from Agrocybe aegerita mushroom.•This enzyme is the first ribotoxin from the Basidiomycetes class.•Ageritin is enzymatically similar to alpha-sarcin, a ribotoxin prototype.•This protein is structurally different from the widely known Ascomycota ribotoxins.•Ageritin inhibits the cell proliferation of several CNS model cell lines via apoptosis. |
|---|---|
| AbstractList | BACKGROUNDSeveral species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pest resistant transgenic plants.RESULTSThe ribotoxin Ageritin has been isolated for the first time from the Basidiomycetes class. The enzyme, characterized by means of its amino acid composition, N-terminal sequence and a circular dichroism, structurally differs from Ascomycota ribotoxin prototype, although it was able, as α-sarcin, to release a specific α-fragment. However, it does not display aspecific ribonucleolytic activity. Ageritin exerts cytotoxicity and cell death promoting effects towards CNS model cell lines (SK-N-BE(2)-C, U-251 and C6), as vinblastine, a plant alkaloid used in cancer therapy. Moreover, our results indicate that Ageritin initially activates caspase-8, whereas caspase-9 cleavage was not detected, demonstrating the involvement of an extrinsic apoptotic pathway.CONCLUSIONSOur findings show that Ageritin is the earliest diverging member of the Ascomycota ribotoxin family, suggesting that ribotoxins are more widely distributed among fungi than previously believed.GENERAL SIGNIFICANCEAgeritin, structurally different from the widely known Ascomycota ribotoxins, with promising anti-cancer properties vs. aggressive brain tumours, has been found from the basidiomycete fungus Agrocybe aegerita. Finally, this finding highlights that the ribotoxin family has divergent members in Basidiomycota phylum, whose structural and functional characterization can give new information on ribotoxin or ribonuclease superfamilies. Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pest resistant transgenic plants.The ribotoxin Ageritin has been isolated for the first time from the Basidiomycetes class. The enzyme, characterized by means of its amino acid composition, N-terminal sequence and a circular dichroism, structurally differs from Ascomycota ribotoxin prototype, although it was able, as α-sarcin, to release a specific α-fragment. However, it does not display aspecific ribonucleolytic activity. Ageritin exerts cytotoxicity and cell death promoting effects towards CNS model cell lines (SK-N-BE(2)-C, U-251 and C6), as vinblastine, a plant alkaloid used in cancer therapy. Moreover, our results indicate that Ageritin initially activates caspase-8, whereas caspase-9 cleavage was not detected, demonstrating the involvement of an extrinsic apoptotic pathway.Our findings show that Ageritin is the earliest diverging member of the Ascomycota ribotoxin family, suggesting that ribotoxins are more widely distributed among fungi than previously believed.Ageritin, structurally different from the widely known Ascomycota ribotoxins, with promising anti-cancer properties vs. aggressive brain tumours, has been found from the basidiomycete fungus Agrocybe aegerita. Finally, this finding highlights that the ribotoxin family has divergent members in Basidiomycota phylum, whose structural and functional characterization can give new information on ribotoxin or ribonuclease superfamilies. Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pest resistant transgenic plants. The ribotoxin Ageritin has been isolated for the first time from the Basidiomycetes class. The enzyme, characterized by means of its amino acid composition, N-terminal sequence and a circular dichroism, structurally differs from Ascomycota ribotoxin prototype, although it was able, as α-sarcin, to release a specific α-fragment. However, it does not display aspecific ribonucleolytic activity. Ageritin exerts cytotoxicity and cell death promoting effects towards CNS model cell lines (SK-N-BE(2)-C, U-251 and C6), as vinblastine, a plant alkaloid used in cancer therapy. Moreover, our results indicate that Ageritin initially activates caspase-8, whereas caspase-9 cleavage was not detected, demonstrating the involvement of an extrinsic apoptotic pathway. Our findings show that Ageritin is the earliest diverging member of the Ascomycota ribotoxin family, suggesting that ribotoxins are more widely distributed among fungi than previously believed. Ageritin, structurally different from the widely known Ascomycota ribotoxins, with promising anti-cancer properties vs. aggressive brain tumours, has been found from the basidiomycete fungus Agrocybe aegerita. Finally, this finding highlights that the ribotoxin family has divergent members in Basidiomycota phylum, whose structural and functional characterization can give new information on ribotoxin or ribonuclease superfamilies. Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a single phosphodiester bond, located at the universally conserved sarcin/ricin loop of the large rRNA leading to inhibition of protein biosynthesis. Clarifying the structure-function relationship in ribotoxins is interesting for their use in human tumour therapy and in construction of pest resistant transgenic plants. The ribotoxin Ageritin has been isolated for the first time from the Basidiomycetes class. The enzyme, characterized by means of its amino acid composition, N-terminal sequence and a circular dichroism, structurally differs from Ascomycota ribotoxin prototype, although it was able, as α-sarcin, to release a specific α-fragment. However, it does not display aspecific ribonucleolytic activity. Ageritin exerts cytotoxicity and cell death promoting effects towards CNS model cell lines (SK-N-BE(2)-C, U-251 and C6), as vinblastine, a plant alkaloid used in cancer therapy. Moreover, our results indicate that Ageritin initially activates caspase-8, whereas caspase-9 cleavage was not detected, demonstrating the involvement of an extrinsic apoptotic pathway. Our findings show that Ageritin is the earliest diverging member of the Ascomycota ribotoxin family, suggesting that ribotoxins are more widely distributed among fungi than previously believed. Ageritin, structurally different from the widely known Ascomycota ribotoxins, with promising anti-cancer properties vs. aggressive brain tumours, has been found from the basidiomycete fungus Agrocybe aegerita. Finally, this finding highlights that the ribotoxin family has divergent members in Basidiomycota phylum, whose structural and functional characterization can give new information on ribotoxin or ribonuclease superfamilies. [Display omitted] •Ageritin is a ribotoxin purified from Agrocybe aegerita mushroom.•This enzyme is the first ribotoxin from the Basidiomycetes class.•Ageritin is enzymatically similar to alpha-sarcin, a ribotoxin prototype.•This protein is structurally different from the widely known Ascomycota ribotoxins.•Ageritin inhibits the cell proliferation of several CNS model cell lines via apoptosis. |
| Author | Di Giuseppe, Antonella M.A. Di Maro, Antimo Ragucci, Sara Landi, Nicola Amici, Adolfo Piccolella, Simona Pacifico, Severina Iglesias, Rosario |
| Author_xml | – sequence: 1 givenname: Nicola surname: Landi fullname: Landi, Nicola organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy – sequence: 2 givenname: Severina surname: Pacifico fullname: Pacifico, Severina organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy – sequence: 3 givenname: Sara surname: Ragucci fullname: Ragucci, Sara organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy – sequence: 4 givenname: Rosario surname: Iglesias fullname: Iglesias, Rosario organization: Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, E-47011 Valladolid, Spain – sequence: 5 givenname: Simona surname: Piccolella fullname: Piccolella, Simona organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy – sequence: 6 givenname: Adolfo surname: Amici fullname: Amici, Adolfo organization: Department of Odontostomatologic and Specialized Clinical Sciences, Polytechnic University of Marche, I-60131 Ancona, Italy – sequence: 7 givenname: Antonella M.A. surname: Di Giuseppe fullname: Di Giuseppe, Antonella M.A. organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy – sequence: 8 givenname: Antimo surname: Di Maro fullname: Di Maro, Antimo email: antimo.dimaro@unina2.it organization: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, I-81100 Caserta, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28232091$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkUtrGzEUhUVJaJy0_6AULbvouHrMM4uCCX1BIFkka6HHlS3jkVJJEzr9D_3Ple1k00UjLgh0v3NA55yjEx88IPSOkiUltP20XSol1-CXjNBuSVgZ_gotaN-xqiekPUELwkld1bRtztB5SltSTjM0r9EZ6xlnZKAL9Od2is46LbML_iPWGxmlzhDd78MLlt5gPeeQwy-nXZ6xTAlSGsFnHCxerQuanb_EdxvA1sWUcXTqgHtsYxhxLgslkzMujLOGDHic0iaGslqtY9CzAizh4CPfoFMrdwnePt0X6P7rl7ur79X1zbcfV6vrSvOhyZWtqVZM6lYBMV35pDZgOTe9ZbQbwPS61mRQVvVDaxstGeM1kcb2jWk4tTW_QB-Ovg8x_JwgZTG6pGG3kx7ClAQrSfG6o4y8iO7zbrqhr7uCvn9CJzWCEQ_RjTLO4jntAlweAR1DShGsKJEecs5Rup2gROyrFVtxrFbsqxWEleFFXP8jfvZ_Qfb5KIOS56ODKJJ24DUYF0FnYYL7v8FfgfrC9A |
| CitedBy_id | crossref_primary_10_3390_molecules27082385 crossref_primary_10_1016_j_ijbiomac_2020_07_267 crossref_primary_10_3390_ijms22168964 crossref_primary_10_1016_j_ijbiomac_2019_11_090 crossref_primary_10_1016_j_bcab_2019_101215 crossref_primary_10_1099_mgen_0_000637 crossref_primary_10_1128_AEM_01549_19 crossref_primary_10_3390_biom13020237 crossref_primary_10_1016_j_foodchem_2022_133655 crossref_primary_10_1016_j_ijbiomac_2019_06_125 crossref_primary_10_1016_j_foodchem_2020_128337 crossref_primary_10_1016_j_ijbiomac_2024_138447 crossref_primary_10_1093_jb_mvab062 crossref_primary_10_1039_C9FO01483G crossref_primary_10_1007_s11101_021_09748_2 crossref_primary_10_1016_j_fbio_2025_106859 crossref_primary_10_1016_j_foodchem_2025_144026 crossref_primary_10_3390_toxins12080524 crossref_primary_10_1016_j_ijbiomac_2022_09_103 crossref_primary_10_1016_j_bbagen_2018_09_010 crossref_primary_10_1002_jsfa_8428 crossref_primary_10_3390_toxins13040263 crossref_primary_10_1080_87559129_2023_2202738 crossref_primary_10_2174_0929867327666200402100157 crossref_primary_10_3390_jof10090602 crossref_primary_10_3390_toxins14020084 crossref_primary_10_1016_j_ijbiomac_2024_135700 crossref_primary_10_1093_jb_mvy113 crossref_primary_10_3390_toxins15010057 crossref_primary_10_3390_toxins14080566 crossref_primary_10_3390_toxins14060403 crossref_primary_10_1016_j_napere_2023_100044 crossref_primary_10_1016_j_abb_2023_109836 crossref_primary_10_3390_jof7090700 crossref_primary_10_1016_j_foodchem_2021_129931 crossref_primary_10_3390_ijms21197158 |
| Cites_doi | 10.1016/j.ijdevneu.2012.12.006 10.1155/2015/376387 10.1038/443758a 10.1016/j.foodchem.2012.02.042 10.1016/j.fct.2016.06.028 10.1111/j.1442-2050.2012.01397.x 10.1016/S0021-9258(18)32977-6 10.1007/s00425-014-2191-2 10.3390/molecules21121627 10.4103/2319-4170.138318 10.1016/S0021-9258(18)45660-8 10.1016/j.smim.2014.03.005 10.1089/jmf.2013.2846 10.1155/2014/805841 10.1007/978-1-62703-290-2_7 10.1016/j.mycres.2007.03.004 10.1002/bip.20853 10.1016/j.phytochem.2006.12.002 10.3314/jjmm.46.11 10.1126/scitranslmed.3004404 10.3732/ajb.1000298 10.1016/0003-2697(82)90347-5 10.1111/j.1469-8137.2006.01936.x 10.1002/jms.102 10.1186/s40694-014-0008-5 10.1111/j.1574-6976.2006.00063.x 10.1021/bi00566a011 10.1016/S0041-0101(99)00103-8 10.1042/bj20030300 10.1002/mnfr.201200316 10.1016/0006-291X(82)91868-X 10.1007/s00425-008-0796-z 10.1016/S0021-9258(19)86062-3 10.1021/np100255u 10.3389/fpls.2016.00631 10.1101/gr.7.11.1041 10.1099/13500872-141-6-1343 10.3390/molecules201019489 10.1016/0167-4838(91)90111-C 10.1017/S0006323198005167 10.1007/s00253-016-7671-9 10.1016/j.ejphar.2009.06.067 10.2174/138920108784567335 10.3390/insects4030339 10.1016/j.bbagen.2016.03.011 10.1016/j.bbapap.2011.06.017 10.1016/j.taap.2012.09.017 |
| ContentType | Journal Article |
| Copyright | 2017 Elsevier B.V. Copyright © 2017 Elsevier B.V. All rights reserved. |
| Copyright_xml | – notice: 2017 Elsevier B.V. – notice: Copyright © 2017 Elsevier B.V. All rights reserved. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7U8 7X8 C1K JXQ 7S9 L.6 |
| DOI | 10.1016/j.bbagen.2017.02.023 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed TOXLINE MEDLINE - Academic Environmental Sciences and Pollution Management Toxline AGRICOLA AGRICOLA - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) TOXLINE MEDLINE - Academic Environmental Sciences and Pollution Management AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | TOXLINE AGRICOLA MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry Biology |
| EISSN | 1872-8006 |
| EndPage | 1121 |
| ExternalDocumentID | 28232091 10_1016_j_bbagen_2017_02_023 S0304416517300776 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 23N 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 8P~ 9JM AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABEFU ABFNM ABGSF ABMAC ABUDA ABXDB ABYKQ ACDAQ ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEHWI AEKER AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CS3 DOVZS EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HVGLF HZ~ IHE J1W KOM LX3 M41 MO0 N9A O-L O9- OAUVE OHT OZT P-8 P-9 PC. Q38 R2- ROL RPZ SBG SCC SDF SDG SDP SES SEW SPCBC SSU SSZ T5K UQL WH7 WUQ XJT XPP ~G- 9DU AAHBH AATTM AAXKI AAYWO AAYXX ABWVN ACLOT ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGQPQ AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP CITATION EFKBS ~HD AGCQF AGRNS BNPGV CGR CUY CVF ECM EIF NPM SSH 7U8 7X8 C1K JXQ 7S9 L.6 |
| ID | FETCH-LOGICAL-c395t-f41cb2ac6be0d7304cdef33d8f2179ed8c4c09bfb896f5ca22340adf85d531f43 |
| ISICitedReferencesCount | 38 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000403733500015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0304-4165 |
| IngestDate | Sun Sep 28 06:02:08 EDT 2025 Sun Sep 28 12:18:13 EDT 2025 Mon Jul 21 05:42:28 EDT 2025 Sat Nov 29 01:38:04 EST 2025 Tue Nov 18 21:02:51 EST 2025 Fri Feb 23 02:34:16 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | Ribotoxins Ribonuclease activity Cytotoxicity Ageritin Agrocybe aegerita Apoptosis |
| Language | English |
| License | Copyright © 2017 Elsevier B.V. All rights reserved. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c395t-f41cb2ac6be0d7304cdef33d8f2179ed8c4c09bfb896f5ca22340adf85d531f43 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 28232091 |
| PQID | 1872579847 |
| PQPubID | 23479 |
| PageCount | 9 |
| ParticipantIDs | proquest_miscellaneous_2000347120 proquest_miscellaneous_1872579847 pubmed_primary_28232091 crossref_citationtrail_10_1016_j_bbagen_2017_02_023 crossref_primary_10_1016_j_bbagen_2017_02_023 elsevier_sciencedirect_doi_10_1016_j_bbagen_2017_02_023 |
| PublicationCentury | 2000 |
| PublicationDate | May 2017 2017-05-00 2017-May 20170501 |
| PublicationDateYYYYMMDD | 2017-05-01 |
| PublicationDate_xml | – month: 05 year: 2017 text: May 2017 |
| PublicationDecade | 2010 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Biochimica et biophysica acta. General subjects |
| PublicationTitleAlternate | Biochim Biophys Acta Gen Subj |
| PublicationYear | 2017 |
| Publisher | Elsevier B.V |
| Publisher_xml | – name: Elsevier B.V |
| References | Fang, Ng (bb0090) 2011; 1815 Liang, Liu, Chen, Sun (bb0130) 2014; 17 Iglesias, Citores, Ragucci, Russo, Di Maro, Ferreras (bb0140) 2016; 1860 Bruns (bb0005) 2006; 443 Ng, Cheung, Wong, Chan, Dan, Pan, Wang, Guan, Chan, Ye, Liu, Xia, Chan (bb0080) 2016 Herrero-Galan, Garcia-Ortega, Olombrada, Lacadena, Del Pozo, Gavilanes, Onaderra (bb0220) 2013; 4 Lacadena, Alvarez-Garcia, Carreras-Sangra, Herrero-Galan, Alegre-Cebollada, Garcia-Ortega, Onaderra, Gavilanes, Martinez del Pozo (bb0215) 2007; 31 Mazet, Vey (bb0235) 1995; 141 Hibbett, Binder, Bischoff, Blackwell, Cannon, Eriksson, Huhndorf, James, Kirk, Lücking, Thorsten Lumbsch, Lutzoni, Matheny, McLaughlin, Powell, Redhead, Schoch, Spatafora, Stalpers, Vilgalys, Aime, Aptroot, Bauer, Begerow, Benny, Castlebury, Crous, Dai, Gams, Geiser, Griffith, Gueidan, Hawksworth, Hestmark, Hosaka, Humber, Hyde, Ironside, Kõljalg, Kurtzman, Larsson, Lichtwardt, Longcore, Miadlikowska, Miller, Moncalvo, Mozley-Standridge, Oberwinkler, Parmasto, Reeb, Rogers, Roux, Ryvarden, Sampaio, Schüßler, Sugiyama, Thorn, Tibell, Untereiner, Walker, Wang, Weir, Weiss, White, Winka, Yao, Zhang (bb0055) 2007; 111 He, Zhou, Pestka (bb0255) 2012; 265 Chan, Moriwaki, De Rosa (bb0180) 2013; 979 Parente, Conforto, Di Maro, Chambery, De Luca, Bolognesi, Iriti, Faoro (bb0135) 2008; 228 Udagawa (bb0035) 2005; 46 Elsayed, El Enshasy, Wadaan, Aziz (bb0070) 2014; 2014 Piccolella, Nocera, Carillo, Woodrow, Greco, Manti, Fiorentino, Pacifico (bb0185) 2016; 95 Endo, Huber, Wool (bb0225) 1983; 258 Idnurm, Meyer (bb0020) 2014; 1 Zhong, Xiao (bb0110) 2009 Pacifico, Scognamiglio, D'Abrosca, Piccolella, Tsafantakis, Gallicchio, Ricci, Fiorentino (bb0175) 2010; 73 Iglesias, Citores, Di Maro, Ferreras (bb0210) 2015; 241 Di Maro, Chambery, Daniele, Casoria, Parente (bb0155) 2007; 68 Aitken, Learmonth (bb0200) 2002 Wright (bb0105) 2001 May, Adams (bb0045) 1997; 7 Di Maro, Ferranti, Mastronicola, Polito, Bolognesi, Stirpe, Malorni, Parente (bb0165) 2001; 36 Blackwell (bb0010) 2011; 98 Girard, McLoughlin, de Kievit, Fernando, Belmonte (bb0030) 2016; 7 Cavalier-Smith (bb0050) 1998; 73 Rodriguez, Lopez-Otin, Barber, Fernandez-Luna, Gonzalez, Mendez (bb0230) 1982; 108 Gasset, Onaderra, Goormaghtigh, Gavilanes (bb0250) 1991; 1080 Ji, Zheng, Ye, Wu, Chen (bb0115) 2013; 26 Leach, Collawn, Fish (bb0170) 1980; 19 Brown, Denning, Gow, Levitz, Netea, White (bb0025) 2012; 4 Chambery, Di Maro, Parente (bb0145) 1973-1982; 69 Blank, Sugiyama, Dekker (bb0150) 1982; 120 Kozarski, Klaus, Jakovljevic, Todorovic, Vunduk, Petrovic, Niksic, Vrvic, van Griensven (bb0075) 2015; 20 Carreras-Sangra, Alvarez-Garcia, Herrero-Galan, Tome, Lacadena, Alegre-Cebollada, Onaderra, Gavilanes, Martinez-Del-Pozo (bb0100) 2008; 9 Gysin, Moore, Proffitt, Deuel, Caldwell, Glaser (bb0270) 1980; 255 Lindahl, Ihrmark, Boberg, Trumbore, Hogberg, Stenlid, Finlay (bb0015) 2007; 173 Kleinkauf-Rocha, Bobermin, Machado Pde, Goncalves, Gottfried, Quincozes-Santos (bb0265) 2013; 31 Martinez-Ruiz, Kao, Davies, Martinez del Pozo (bb0240) 1999; 37 Zhao, Sun, Tong, Qi (bb0125) 2003; 374 Gasset (bb0205) 1997 Valverde, Hernandez-Perez, Paredes-Lopez (bb0065) 2015; 2015 Olombrada, Medina, Budia, Gavilanes, Martinez-Del-Pozo, Garcia-Ortega (bb0245) 2016 Wasser (bb0060) 2014; 37 Alvarez-Garcia, Diago-Navarro, Herrero-Galan, Garcia-Ortega, Lopez-Villarejo, Olmo, Diaz-Orejas, Gavilanes, Martinez-del-Pozo (bb0260) 2011; 1814 Dosi, Carusone, Chambery, Severino, Parente, Di Maro (bb0190) 2012; 133 Bolognesi, Bortolotti, Maiello, Battelli, Polito (bb0095) 2016; 21 Endo, Mitsui, Motizuki, Tsurugi (bb0160) 1987; 262 Whitmore, Wallace (bb0195) 2008; 89 Matassov, Kagan, Leblanc, Sikorska, Zakeri (bb0280) 2004; 282 Gajos, Hilszczańska (bb0040) 2013; 8 Ardelt, Ardelt, Darzynkiewicz (bb0085) 2009; 625 Salvesen, Walsh (bb0275) 2014; 26 Chen, Jiang, Jin, Yin, Yu, Lan, Cui, Liang, Wong, Guo, Sun (bb0120) 2012; 56 Ardelt (10.1016/j.bbagen.2017.02.023_bb0085) 2009; 625 Wright (10.1016/j.bbagen.2017.02.023_bb0105) 2001 Chambery (10.1016/j.bbagen.2017.02.023_bb0145) 1973; 69 He (10.1016/j.bbagen.2017.02.023_bb0255) 2012; 265 Alvarez-Garcia (10.1016/j.bbagen.2017.02.023_bb0260) 2011; 1814 Rodriguez (10.1016/j.bbagen.2017.02.023_bb0230) 1982; 108 Olombrada (10.1016/j.bbagen.2017.02.023_bb0245) 2016 Idnurm (10.1016/j.bbagen.2017.02.023_bb0020) 2014; 1 Zhao (10.1016/j.bbagen.2017.02.023_bb0125) 2003; 374 Gasset (10.1016/j.bbagen.2017.02.023_bb0205) 1997 Gasset (10.1016/j.bbagen.2017.02.023_bb0250) 1991; 1080 Kozarski (10.1016/j.bbagen.2017.02.023_bb0075) 2015; 20 Ng (10.1016/j.bbagen.2017.02.023_bb0080) 2016 May (10.1016/j.bbagen.2017.02.023_bb0045) 1997; 7 Hibbett (10.1016/j.bbagen.2017.02.023_bb0055) 2007; 111 Herrero-Galan (10.1016/j.bbagen.2017.02.023_bb0220) 2013; 4 Martinez-Ruiz (10.1016/j.bbagen.2017.02.023_bb0240) 1999; 37 Zhong (10.1016/j.bbagen.2017.02.023_bb0110) 2009 Udagawa (10.1016/j.bbagen.2017.02.023_bb0035) 2005; 46 Di Maro (10.1016/j.bbagen.2017.02.023_bb0155) 2007; 68 Wasser (10.1016/j.bbagen.2017.02.023_bb0060) 2014; 37 Parente (10.1016/j.bbagen.2017.02.023_bb0135) 2008; 228 Pacifico (10.1016/j.bbagen.2017.02.023_bb0175) 2010; 73 Kleinkauf-Rocha (10.1016/j.bbagen.2017.02.023_bb0265) 2013; 31 Fang (10.1016/j.bbagen.2017.02.023_bb0090) 2011; 1815 Iglesias (10.1016/j.bbagen.2017.02.023_bb0140) 2016; 1860 Endo (10.1016/j.bbagen.2017.02.023_bb0160) 1987; 262 Chan (10.1016/j.bbagen.2017.02.023_bb0180) 2013; 979 Carreras-Sangra (10.1016/j.bbagen.2017.02.023_bb0100) 2008; 9 Chen (10.1016/j.bbagen.2017.02.023_bb0120) 2012; 56 Blank (10.1016/j.bbagen.2017.02.023_bb0150) 1982; 120 Gajos (10.1016/j.bbagen.2017.02.023_bb0040) 2013; 8 Di Maro (10.1016/j.bbagen.2017.02.023_bb0165) 2001; 36 Leach (10.1016/j.bbagen.2017.02.023_bb0170) 1980; 19 Mazet (10.1016/j.bbagen.2017.02.023_bb0235) 1995; 141 Ji (10.1016/j.bbagen.2017.02.023_bb0115) 2013; 26 Matassov (10.1016/j.bbagen.2017.02.023_bb0280) 2004; 282 Liang (10.1016/j.bbagen.2017.02.023_bb0130) 2014; 17 Dosi (10.1016/j.bbagen.2017.02.023_bb0190) 2012; 133 Salvesen (10.1016/j.bbagen.2017.02.023_bb0275) 2014; 26 Valverde (10.1016/j.bbagen.2017.02.023_bb0065) 2015; 2015 Aitken (10.1016/j.bbagen.2017.02.023_bb0200) 2002 Brown (10.1016/j.bbagen.2017.02.023_bb0025) 2012; 4 Elsayed (10.1016/j.bbagen.2017.02.023_bb0070) 2014; 2014 Endo (10.1016/j.bbagen.2017.02.023_bb0225) 1983; 258 Gysin (10.1016/j.bbagen.2017.02.023_bb0270) 1980; 255 Lindahl (10.1016/j.bbagen.2017.02.023_bb0015) 2007; 173 Girard (10.1016/j.bbagen.2017.02.023_bb0030) 2016; 7 Lacadena (10.1016/j.bbagen.2017.02.023_bb0215) 2007; 31 Bolognesi (10.1016/j.bbagen.2017.02.023_bb0095) 2016; 21 Piccolella (10.1016/j.bbagen.2017.02.023_bb0185) 2016; 95 Iglesias (10.1016/j.bbagen.2017.02.023_bb0210) 2015; 241 Blackwell (10.1016/j.bbagen.2017.02.023_bb0010) 2011; 98 Cavalier-Smith (10.1016/j.bbagen.2017.02.023_bb0050) 1998; 73 Whitmore (10.1016/j.bbagen.2017.02.023_bb0195) 2008; 89 Bruns (10.1016/j.bbagen.2017.02.023_bb0005) 2006; 443 |
| References_xml | – volume: 26 start-page: 246 year: 2014 end-page: 252 ident: bb0275 article-title: Functions of caspase 8: the identified and the mysterious publication-title: Semin. Immunol. – start-page: 58 year: 1997 end-page: 60 ident: bb0205 article-title: Toxins affecting protein synthesis publication-title: Guidebook to Protein Toxins and Their Use in Cell Biology, OUP/Sambrook and Tooze Publications – volume: 31 start-page: 212 year: 2007 end-page: 237 ident: bb0215 article-title: Fungal ribotoxins: molecular dissection of a family of natural killers publication-title: FEMS Microbiol. Rev. – volume: 255 start-page: 1515 year: 1980 end-page: 1520 ident: bb0270 article-title: Regulation of the synthesis of S-100 protein in rat glial cells publication-title: J. Biol. Chem. – volume: 374 start-page: 321 year: 2003 end-page: 327 ident: bb0125 article-title: An antitumour lectin from the edible mushroom publication-title: Biochem. J. – volume: 241 start-page: 421 year: 2015 end-page: 433 ident: bb0210 article-title: Biological activities of the antiviral protein BE27 from sugar beet ( publication-title: Planta – volume: 8 start-page: 1837 year: 2013 end-page: 1847 ident: bb0040 article-title: Research on truffles: scientific journals analysis publication-title: Sci. Res. Essays – volume: 36 start-page: 38 year: 2001 end-page: 46 ident: bb0165 article-title: Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation publication-title: J. Mass Spectrom. – volume: 108 start-page: 315 year: 1982 end-page: 321 ident: bb0230 article-title: Amino acid sequence homologies in alfa-sarcin, restrictocin and mitogillin publication-title: Biochem. Biophys. Res. Commun. – volume: 262 start-page: 5908 year: 1987 end-page: 5912 ident: bb0160 article-title: The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins publication-title: J. Biol. Chem. – volume: 37 start-page: 345 year: 2014 end-page: 356 ident: bb0060 article-title: Medicinal mushroom science: current perspectives, advances, evidences, and challenges publication-title: Biomed. J. – year: 2001 ident: bb0105 article-title: Mediterranean Vegetables: A Cook's ABC of Vegetables and Their Preparation in Spain, France, Italy, Greece, Turkey, the Middle East, and North Africa with More Than 200 Authentic Recipes for the Home Cook – volume: 68 start-page: 767 year: 2007 end-page: 776 ident: bb0155 article-title: Isolation and characterization of heterotepalins, type 1 ribosome-inactivating proteins from publication-title: Phytochemistry – volume: 1814 start-page: 1377 year: 2011 end-page: 1382 ident: bb0260 article-title: The ribonucleolytic activity of the ribotoxin alpha-sarcin is not essential for publication-title: Biochim. Biophys. Acta – volume: 56 start-page: 1729 year: 2012 end-page: 1738 ident: bb0120 article-title: Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom publication-title: Mol. Nutr. Food Res. – volume: 20 start-page: 19489 year: 2015 end-page: 19525 ident: bb0075 article-title: Antioxidants of edible mushrooms publication-title: Molecules – volume: 173 start-page: 611 year: 2007 end-page: 620 ident: bb0015 article-title: Spatial separation of litter decomposition and mycorrhizal nitrogen uptake in a boreal forest publication-title: New Phytol. – volume: 46 start-page: 11 year: 2005 end-page: 15 ident: bb0035 article-title: Fungal spoilage of foods and its risk assessment publication-title: Jpn. J. Med. Mycol. – volume: 95 start-page: 64 year: 2016 end-page: 74 ident: bb0185 article-title: An apolar publication-title: Food Chem. Toxicol. – volume: 7 start-page: 631 year: 2016 ident: bb0030 article-title: Integrating large-scale data and RNA technology to protect crops from fungal pathogens publication-title: Front. Plant Sci. – volume: 19 start-page: 5734 year: 1980 end-page: 5741 ident: bb0170 article-title: Behavior of glycopolypeptides with empirical molecular weight estimation methods. 1. In sodium dodecyl sulfate publication-title: Biochemistry – volume: 98 start-page: 426 year: 2011 end-page: 438 ident: bb0010 article-title: The fungi: 1, 2, 3 … 5.1 million species? publication-title: Am. J. Bot. – volume: 443 start-page: 758 year: 2006 end-page: 761 ident: bb0005 article-title: Evolutionary biology: a kingdom revised publication-title: Nature – volume: 2015 start-page: 376387 year: 2015 ident: bb0065 article-title: Edible mushrooms: improving human health and promoting quality life publication-title: Int. J. Microbiol. – volume: 73 start-page: 1973 year: 2010 end-page: 1978 ident: bb0175 article-title: Spectroscopic characterization and antiproliferative activity on HepG2 human hepatoblastoma cells of flavonoid C-glycosides from publication-title: J. Nat. Prod. – volume: 4 start-page: 165rv113 year: 2012 ident: bb0025 article-title: Hidden killers: human fungal infections publication-title: Sci. Transl. Med. – volume: 1815 start-page: 65 year: 2011 end-page: 74 ident: bb0090 article-title: Ribonucleases of different origins with a wide spectrum of medicinal applications publication-title: Biochim. Biophys. Acta – volume: 37 start-page: 1549 year: 1999 end-page: 1563 ident: bb0240 article-title: Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed publication-title: Toxicon – volume: 4 start-page: 339 year: 2013 end-page: 356 ident: bb0220 article-title: Hirsutellin A: a paradigmatic example of the insecticidal function of fungal ribotoxins publication-title: Insects – start-page: 79 year: 2009 end-page: 150 ident: bb0110 article-title: Secondary metabolites from higher fungi: discovery, bioactivity, and bioproduction publication-title: Biotechnology in China I. From Bioreaction to Bioseparation and Bioremediation – volume: 69 year: 1973-1982 ident: bb0145 article-title: Primary structure and glycan moiety characterization of PD-Ss, type 1 ribosome-inactivating proteins from publication-title: Phytochemistry – volume: 133 start-page: 1646 year: 2012 end-page: 1652 ident: bb0190 article-title: Rapid primary structure determination of myoglobins by a complementary approach based on mass spectrometry and Edman degradation publication-title: Food Chem. – volume: 1860 start-page: 1256 year: 2016 end-page: 1264 ident: bb0140 article-title: Biological and antipathogenic activities of ribosome-inactivating proteins from publication-title: Biochim. Biophys. Acta – volume: 17 start-page: 439 year: 2014 end-page: 446 ident: bb0130 article-title: Antitumor activity of the protein and small molecule component fractions from publication-title: J. Med. Food – volume: 625 start-page: 181 year: 2009 end-page: 189 ident: bb0085 article-title: Ribonucleases as potential modalities in anticancer therapy publication-title: Eur. J. Pharmacol. – volume: 31 start-page: 165 year: 2013 end-page: 170 ident: bb0265 article-title: Lipoic acid increases glutamate uptake, glutamine synthetase activity and glutathione content in C6 astrocyte cell line publication-title: Int. J. Dev. Neurosci. – volume: 21 year: 2016 ident: bb0095 article-title: Ribosome-inactivating proteins from plants: a historical overview publication-title: Molecules – volume: 7 start-page: 1041 year: 1997 end-page: 1044 ident: bb0045 article-title: The importance of fungi to man publication-title: Genome Res. – volume: 228 start-page: 963 year: 2008 end-page: 975 ident: bb0135 article-title: Type 1 ribosome-inactivating proteins from publication-title: Planta – volume: 265 start-page: 10 year: 2012 end-page: 18 ident: bb0255 article-title: Mechanisms for ribotoxin-induced ribosomal RNA cleavage publication-title: Toxicol. Appl. Pharmacol. – year: 2016 ident: bb0080 article-title: Fungal proteinaceous compounds with multiple biological activities publication-title: Appl. Microbiol. Biotechnol. – volume: 258 start-page: 2662 year: 1983 end-page: 2667 ident: bb0225 article-title: The ribonuclease activity of the cytotoxin alpha-sarcin. The characteristics of the enzymatic activity of alpha-sarcin with ribosomes and ribonucleic acids as substrates publication-title: J. Biol. Chem. – volume: 1080 start-page: 51 year: 1991 end-page: 58 ident: bb0250 article-title: Acid phospholipid vesicles produce conformational changes on the antitumour protein alpha-sarcin publication-title: Biochim. Biophys. Acta – volume: 141 start-page: 1343 year: 1995 end-page: 1348 ident: bb0235 article-title: Hirsutellin A, a toxic protein produced in vitro by publication-title: Microbiology – volume: 282 start-page: 1 year: 2004 end-page: 17 ident: bb0280 article-title: Measurement of apoptosis by DNA fragmentation publication-title: Methods Mol. Biol. – volume: 979 start-page: 65 year: 2013 end-page: 70 ident: bb0180 article-title: Detection of necrosis by release of lactate dehydrogenase activity publication-title: Methods Mol. Biol. – start-page: 457 year: 2002 end-page: 458 ident: bb0200 article-title: Performic acid oxidation publication-title: The Protein Protocols Handbook – volume: 73 start-page: 203 year: 1998 end-page: 266 ident: bb0050 article-title: A revised six-kingdom system of life publication-title: Biol. Rev. Camb. Philos. Soc. – volume: 111 start-page: 509 year: 2007 end-page: 547 ident: bb0055 article-title: A higher-level phylogenetic classification of the fungi publication-title: Mycol. Res. – volume: 120 start-page: 267 year: 1982 end-page: 275 ident: bb0150 article-title: Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels publication-title: Anal. Biochem. – volume: 1 start-page: 1 year: 2014 end-page: 2 ident: bb0020 article-title: Welcome to fungal biology and biotechnology publication-title: Fungal Biol. Biotechnol. – volume: 2014 start-page: 805841 year: 2014 ident: bb0070 article-title: Mushrooms: a potential natural source of anti-inflammatory compounds for medical applications publication-title: Mediat. Inflamm. – volume: 26 start-page: 859 year: 2013 end-page: 863 ident: bb0115 article-title: polysaccharide combined with chemotherapy improves tumor necrosis factor-alpha and interferon-gamma levels in rat esophageal carcinoma publication-title: Dis. Esophagus – volume: 9 start-page: 153 year: 2008 end-page: 160 ident: bb0100 article-title: The therapeutic potential of fungal ribotoxins publication-title: Curr. Pharm. Biotechnol. – year: 2016 ident: bb0245 article-title: Characterization of a new toxin from the entomopathogenic fungus publication-title: Biol. Chem. – volume: 89 start-page: 392 year: 2008 end-page: 400 ident: bb0195 article-title: Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases publication-title: Biopolymers – volume: 31 start-page: 165 year: 2013 ident: 10.1016/j.bbagen.2017.02.023_bb0265 article-title: Lipoic acid increases glutamate uptake, glutamine synthetase activity and glutathione content in C6 astrocyte cell line publication-title: Int. J. Dev. Neurosci. doi: 10.1016/j.ijdevneu.2012.12.006 – year: 2001 ident: 10.1016/j.bbagen.2017.02.023_bb0105 – volume: 2015 start-page: 376387 year: 2015 ident: 10.1016/j.bbagen.2017.02.023_bb0065 article-title: Edible mushrooms: improving human health and promoting quality life publication-title: Int. J. Microbiol. doi: 10.1155/2015/376387 – volume: 443 start-page: 758 year: 2006 ident: 10.1016/j.bbagen.2017.02.023_bb0005 article-title: Evolutionary biology: a kingdom revised publication-title: Nature doi: 10.1038/443758a – volume: 133 start-page: 1646 year: 2012 ident: 10.1016/j.bbagen.2017.02.023_bb0190 article-title: Rapid primary structure determination of myoglobins by a complementary approach based on mass spectrometry and Edman degradation publication-title: Food Chem. doi: 10.1016/j.foodchem.2012.02.042 – start-page: 79 year: 2009 ident: 10.1016/j.bbagen.2017.02.023_bb0110 article-title: Secondary metabolites from higher fungi: discovery, bioactivity, and bioproduction – volume: 95 start-page: 64 year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0185 article-title: An apolar Pistacia lentiscus L. leaf extract: GC-MS metabolic profiling and evaluation of cytotoxicity and apoptosis inducing effects on SH-SY5Y and SK-N-BE(2)C cell lines publication-title: Food Chem. Toxicol. doi: 10.1016/j.fct.2016.06.028 – volume: 26 start-page: 859 year: 2013 ident: 10.1016/j.bbagen.2017.02.023_bb0115 article-title: Agrocybe aegerita polysaccharide combined with chemotherapy improves tumor necrosis factor-alpha and interferon-gamma levels in rat esophageal carcinoma publication-title: Dis. Esophagus doi: 10.1111/j.1442-2050.2012.01397.x – volume: 258 start-page: 2662 year: 1983 ident: 10.1016/j.bbagen.2017.02.023_bb0225 article-title: The ribonuclease activity of the cytotoxin alpha-sarcin. The characteristics of the enzymatic activity of alpha-sarcin with ribosomes and ribonucleic acids as substrates publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)32977-6 – volume: 241 start-page: 421 year: 2015 ident: 10.1016/j.bbagen.2017.02.023_bb0210 article-title: Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.) publication-title: Planta doi: 10.1007/s00425-014-2191-2 – volume: 21 year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0095 article-title: Ribosome-inactivating proteins from plants: a historical overview publication-title: Molecules doi: 10.3390/molecules21121627 – volume: 37 start-page: 345 year: 2014 ident: 10.1016/j.bbagen.2017.02.023_bb0060 article-title: Medicinal mushroom science: current perspectives, advances, evidences, and challenges publication-title: Biomed. J. doi: 10.4103/2319-4170.138318 – volume: 262 start-page: 5908 year: 1987 ident: 10.1016/j.bbagen.2017.02.023_bb0160 article-title: The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)45660-8 – volume: 26 start-page: 246 year: 2014 ident: 10.1016/j.bbagen.2017.02.023_bb0275 article-title: Functions of caspase 8: the identified and the mysterious publication-title: Semin. Immunol. doi: 10.1016/j.smim.2014.03.005 – volume: 17 start-page: 439 year: 2014 ident: 10.1016/j.bbagen.2017.02.023_bb0130 article-title: Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production publication-title: J. Med. Food doi: 10.1089/jmf.2013.2846 – volume: 1815 start-page: 65 year: 2011 ident: 10.1016/j.bbagen.2017.02.023_bb0090 article-title: Ribonucleases of different origins with a wide spectrum of medicinal applications publication-title: Biochim. Biophys. Acta – volume: 8 start-page: 1837 year: 2013 ident: 10.1016/j.bbagen.2017.02.023_bb0040 article-title: Research on truffles: scientific journals analysis publication-title: Sci. Res. Essays – volume: 2014 start-page: 805841 year: 2014 ident: 10.1016/j.bbagen.2017.02.023_bb0070 article-title: Mushrooms: a potential natural source of anti-inflammatory compounds for medical applications publication-title: Mediat. Inflamm. doi: 10.1155/2014/805841 – volume: 282 start-page: 1 year: 2004 ident: 10.1016/j.bbagen.2017.02.023_bb0280 article-title: Measurement of apoptosis by DNA fragmentation publication-title: Methods Mol. Biol. – volume: 979 start-page: 65 year: 2013 ident: 10.1016/j.bbagen.2017.02.023_bb0180 article-title: Detection of necrosis by release of lactate dehydrogenase activity publication-title: Methods Mol. Biol. doi: 10.1007/978-1-62703-290-2_7 – start-page: 58 year: 1997 ident: 10.1016/j.bbagen.2017.02.023_bb0205 article-title: Toxins affecting protein synthesis – volume: 111 start-page: 509 year: 2007 ident: 10.1016/j.bbagen.2017.02.023_bb0055 article-title: A higher-level phylogenetic classification of the fungi publication-title: Mycol. Res. doi: 10.1016/j.mycres.2007.03.004 – volume: 89 start-page: 392 year: 2008 ident: 10.1016/j.bbagen.2017.02.023_bb0195 article-title: Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases publication-title: Biopolymers doi: 10.1002/bip.20853 – volume: 68 start-page: 767 year: 2007 ident: 10.1016/j.bbagen.2017.02.023_bb0155 article-title: Isolation and characterization of heterotepalins, type 1 ribosome-inactivating proteins from Phytolacca heterotepala leaves publication-title: Phytochemistry doi: 10.1016/j.phytochem.2006.12.002 – volume: 46 start-page: 11 year: 2005 ident: 10.1016/j.bbagen.2017.02.023_bb0035 article-title: Fungal spoilage of foods and its risk assessment publication-title: Jpn. J. Med. Mycol. doi: 10.3314/jjmm.46.11 – volume: 4 start-page: 165rv113 year: 2012 ident: 10.1016/j.bbagen.2017.02.023_bb0025 article-title: Hidden killers: human fungal infections publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3004404 – volume: 98 start-page: 426 year: 2011 ident: 10.1016/j.bbagen.2017.02.023_bb0010 article-title: The fungi: 1, 2, 3 … 5.1 million species? publication-title: Am. J. Bot. doi: 10.3732/ajb.1000298 – volume: 120 start-page: 267 year: 1982 ident: 10.1016/j.bbagen.2017.02.023_bb0150 article-title: Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels publication-title: Anal. Biochem. doi: 10.1016/0003-2697(82)90347-5 – volume: 173 start-page: 611 year: 2007 ident: 10.1016/j.bbagen.2017.02.023_bb0015 article-title: Spatial separation of litter decomposition and mycorrhizal nitrogen uptake in a boreal forest publication-title: New Phytol. doi: 10.1111/j.1469-8137.2006.01936.x – volume: 36 start-page: 38 year: 2001 ident: 10.1016/j.bbagen.2017.02.023_bb0165 article-title: Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation publication-title: J. Mass Spectrom. doi: 10.1002/jms.102 – start-page: 457 year: 2002 ident: 10.1016/j.bbagen.2017.02.023_bb0200 article-title: Performic acid oxidation – volume: 1 start-page: 1 year: 2014 ident: 10.1016/j.bbagen.2017.02.023_bb0020 article-title: Welcome to fungal biology and biotechnology publication-title: Fungal Biol. Biotechnol. doi: 10.1186/s40694-014-0008-5 – volume: 69 issue: 2008 year: 1973 ident: 10.1016/j.bbagen.2017.02.023_bb0145 article-title: Primary structure and glycan moiety characterization of PD-Ss, type 1 ribosome-inactivating proteins from Phytolacca dioica L. seeds, by precursor ion discovery on a Q-TOF mass spectrometer publication-title: Phytochemistry – year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0245 article-title: Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin publication-title: Biol. Chem. – volume: 31 start-page: 212 year: 2007 ident: 10.1016/j.bbagen.2017.02.023_bb0215 article-title: Fungal ribotoxins: molecular dissection of a family of natural killers publication-title: FEMS Microbiol. Rev. doi: 10.1111/j.1574-6976.2006.00063.x – volume: 19 start-page: 5734 year: 1980 ident: 10.1016/j.bbagen.2017.02.023_bb0170 article-title: Behavior of glycopolypeptides with empirical molecular weight estimation methods. 1. In sodium dodecyl sulfate publication-title: Biochemistry doi: 10.1021/bi00566a011 – volume: 37 start-page: 1549 year: 1999 ident: 10.1016/j.bbagen.2017.02.023_bb0240 article-title: Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed publication-title: Toxicon doi: 10.1016/S0041-0101(99)00103-8 – volume: 374 start-page: 321 year: 2003 ident: 10.1016/j.bbagen.2017.02.023_bb0125 article-title: An antitumour lectin from the edible mushroom Agrocybe aegerita publication-title: Biochem. J. doi: 10.1042/bj20030300 – volume: 56 start-page: 1729 year: 2012 ident: 10.1016/j.bbagen.2017.02.023_bb0120 article-title: Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom Agrocybe aegerita publication-title: Mol. Nutr. Food Res. doi: 10.1002/mnfr.201200316 – volume: 108 start-page: 315 year: 1982 ident: 10.1016/j.bbagen.2017.02.023_bb0230 article-title: Amino acid sequence homologies in alfa-sarcin, restrictocin and mitogillin publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/0006-291X(82)91868-X – volume: 228 start-page: 963 year: 2008 ident: 10.1016/j.bbagen.2017.02.023_bb0135 article-title: Type 1 ribosome-inactivating proteins from Phytolacca dioica L. leaves: differential seasonal and age expression, and cellular localization publication-title: Planta doi: 10.1007/s00425-008-0796-z – volume: 255 start-page: 1515 year: 1980 ident: 10.1016/j.bbagen.2017.02.023_bb0270 article-title: Regulation of the synthesis of S-100 protein in rat glial cells publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)86062-3 – volume: 73 start-page: 1973 year: 2010 ident: 10.1016/j.bbagen.2017.02.023_bb0175 article-title: Spectroscopic characterization and antiproliferative activity on HepG2 human hepatoblastoma cells of flavonoid C-glycosides from Petrorhagia velutina publication-title: J. Nat. Prod. doi: 10.1021/np100255u – volume: 7 start-page: 631 year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0030 article-title: Integrating large-scale data and RNA technology to protect crops from fungal pathogens publication-title: Front. Plant Sci. doi: 10.3389/fpls.2016.00631 – volume: 7 start-page: 1041 year: 1997 ident: 10.1016/j.bbagen.2017.02.023_bb0045 article-title: The importance of fungi to man publication-title: Genome Res. doi: 10.1101/gr.7.11.1041 – volume: 141 start-page: 1343 issue: Pt 6 year: 1995 ident: 10.1016/j.bbagen.2017.02.023_bb0235 article-title: Hirsutellin A, a toxic protein produced in vitro by Hirsutella thompsonii publication-title: Microbiology doi: 10.1099/13500872-141-6-1343 – volume: 20 start-page: 19489 year: 2015 ident: 10.1016/j.bbagen.2017.02.023_bb0075 article-title: Antioxidants of edible mushrooms publication-title: Molecules doi: 10.3390/molecules201019489 – volume: 1080 start-page: 51 year: 1991 ident: 10.1016/j.bbagen.2017.02.023_bb0250 article-title: Acid phospholipid vesicles produce conformational changes on the antitumour protein alpha-sarcin publication-title: Biochim. Biophys. Acta doi: 10.1016/0167-4838(91)90111-C – volume: 73 start-page: 203 year: 1998 ident: 10.1016/j.bbagen.2017.02.023_bb0050 article-title: A revised six-kingdom system of life publication-title: Biol. Rev. Camb. Philos. Soc. doi: 10.1017/S0006323198005167 – year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0080 article-title: Fungal proteinaceous compounds with multiple biological activities publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-016-7671-9 – volume: 625 start-page: 181 year: 2009 ident: 10.1016/j.bbagen.2017.02.023_bb0085 article-title: Ribonucleases as potential modalities in anticancer therapy publication-title: Eur. J. Pharmacol. doi: 10.1016/j.ejphar.2009.06.067 – volume: 9 start-page: 153 year: 2008 ident: 10.1016/j.bbagen.2017.02.023_bb0100 article-title: The therapeutic potential of fungal ribotoxins publication-title: Curr. Pharm. Biotechnol. doi: 10.2174/138920108784567335 – volume: 4 start-page: 339 year: 2013 ident: 10.1016/j.bbagen.2017.02.023_bb0220 article-title: Hirsutellin A: a paradigmatic example of the insecticidal function of fungal ribotoxins publication-title: Insects doi: 10.3390/insects4030339 – volume: 1860 start-page: 1256 year: 2016 ident: 10.1016/j.bbagen.2017.02.023_bb0140 article-title: Biological and antipathogenic activities of ribosome-inactivating proteins from Phytolacca dioica L publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbagen.2016.03.011 – volume: 1814 start-page: 1377 year: 2011 ident: 10.1016/j.bbagen.2017.02.023_bb0260 article-title: The ribonucleolytic activity of the ribotoxin alpha-sarcin is not essential for in vitro protein biosynthesis inhibition publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbapap.2011.06.017 – volume: 265 start-page: 10 year: 2012 ident: 10.1016/j.bbagen.2017.02.023_bb0255 article-title: Mechanisms for ribotoxin-induced ribosomal RNA cleavage publication-title: Toxicol. Appl. Pharmacol. doi: 10.1016/j.taap.2012.09.017 |
| SSID | ssj0000595 |
| Score | 2.3868637 |
| Snippet | Several species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the cleavage of a... BACKGROUNDSeveral species belonging to Ascomycota phylum produce extracellular ribonucleases, known as ribotoxins, which exhibit RNase activity through the... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 1113 |
| SubjectTerms | Agaricales - chemistry Ageritin Agrocybe - chemistry Agrocybe aegerita amino acid composition Amino Acid Sequence antineoplastic activity Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Ascomycota Basidiomycota - chemistry brain Caspase 8 - metabolism Caspase 9 - metabolism caspase-9 Cell Line, Tumor circular dichroism spectroscopy Cytotoxicity Endoribonucleases - metabolism Fungal Proteins - metabolism genetically modified organisms Humans mushrooms neoplasms pest resistance Protein Biosynthesis - drug effects protein synthesis Ribonuclease activity ribonucleases Ribonucleases - chemistry Ribonucleases - pharmacology ribosomal RNA Ribosomes - chemistry Ribosomes - metabolism Ribotoxins ricin Ricin - metabolism RNA, Ribosomal - metabolism structure-activity relationships therapeutics vinblastine |
| Title | Purification, characterization and cytotoxicity assessment of Ageritin: The first ribotoxin from the basidiomycete mushroom Agrocybe aegerita |
| URI | https://dx.doi.org/10.1016/j.bbagen.2017.02.023 https://www.ncbi.nlm.nih.gov/pubmed/28232091 https://www.proquest.com/docview/1872579847 https://www.proquest.com/docview/2000347120 |
| Volume | 1861 |
| WOSCitedRecordID | wos000403733500015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1872-8006 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000595 issn: 0304-4165 databaseCode: AIEXJ dateStart: 19950118 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLa2DgQvCAaDcZmMxFvJlObS2LxN0xCgaarGQH2LbMfeMq1J1abV-iP4d_wgju3YXQfV4AGpiqr4oiTni8_xyTnfQeid4SkTKgsyUB5BkrF-wApOA54wAdqH8sxQKX0_zk5OyHBIBxsbP10uzPwqqypyfU3H_1XUcA6ErVNn_0HcflI4Af9B6HAEscPxrwQ_mE10-I-N2oAHKDwls824tJlsi6Zu6utSaCOceXZOY5eeS010VLl4DFWCgdidlNwMqJYJKaAAy6KsRwsBhnd3NJteaCscxoNOXOgQDmlmYivfjctaXJSaoqArmy4va-tZYZrTg-07EuzudMa1f8jb-8c6-cYD12uSNqLQeHu_yrnOZPRtp-x8JkTpvN7-FTi_ktPSZrGd1lM2sXFozvEBytSHGbqErxDQ1bOlJpaLeb93A7Zpd9C0TmG7QMPSHv9Rc1gnxuU-59BNE-P2MkPmGq10h0c_HhngwFY1jkJbaewWY7dr2kRbUZZS0kFbB5-Phl-WFkJqqgH5G3ApnSbu8PcL0ITV7ZTrrKd1uyNjJZ09Ro_a7Q0-sLB8gjZktY3u24Kni2304NDVF3yKftwE6nt8G6YYRI5vwhQvYYprhR1MP2AAKTYgxR6kWIMUA0jxCkixAyl2IMUOpM_Qt49HZ4efgrY4SCBimjaBSnqCR0z0uQwLUFOJKKSK44Io2GRTWRCRiJByxQntq1QwMIOTkBWKpAWoHZXEO6hT1ZV8gTCVSvFUcZ4SrcJSRllEQ5FIoghhWbSLYvfQc9Ey5-sCLle5C5G8zK3Uci21PIzgF--iwI8aW-aYO_pnTp55a_1aqzYHiN4x8q0Tfw4y1F_8WCXr2TTvkQxUMgULdH2fyHBUwVId7qLnFjv-eh3sXq5teYUeLt_N16jTTGbyDbon5k05neyhzWxI9lr4_wKqcfKR |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Purification%2C+characterization+and+cytotoxicity+assessment+of+Ageritin%3A+The+first+ribotoxin+from+the+basidiomycete+mushroom+Agrocybe+aegerita&rft.jtitle=Biochimica+et+biophysica+acta.+General+subjects&rft.au=Landi%2C+Nicola&rft.au=Pacifico%2C+Severina&rft.au=Ragucci%2C+Sara&rft.au=Iglesias%2C+Rosario&rft.date=2017-05-01&rft.issn=0304-4165&rft.volume=1861&rft.issue=5+Pt+A&rft.spage=1113&rft_id=info:doi/10.1016%2Fj.bbagen.2017.02.023&rft_id=info%3Apmid%2F28232091&rft.externalDocID=28232091 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0304-4165&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0304-4165&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0304-4165&client=summon |