Standardized method for mechanistic modeling of multimodal anion exchange chromatography in flow through operation

•Hydrophobic interaction chromatography (HIC) isotherms employed for anionic multimodal modeling.•Model parameters analytically determined using negative linear gradient elution (LGE).•Standardized modeling workflow validated for four different antibody formats. Multimodal chromatography offers an i...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 1690; p. 463789
Main Authors:	Hess, Rudger, Yun, Doil, Saleh, David, Briskot, Till, Grosch, Jan-Hendrik, Wang, Gang, Schwab, Thomas, Hubbuch, Jürgen
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 08.02.2023
Subjects:	adsorption Analytical parameter determination anion exchange chromatography antibodies Antibodies, Monoclonal - chemistry biopharmaceuticals Chromatography, Ion Exchange - methods Computer Simulation hydrophobicity immunoglobulin G Mixed-mode gradient elution Model calibration and validation parameter uncertainty Parameter uncertainty assessment prediction Sodium Chloride sorption isotherms Therapeutic antibody Analytical parameter determination Parameter uncertainty assessment Therapeutic antibody Mixed-mode gradient elution Model calibration and validation
ISSN:	0021-9673, 1873-3778, 1873-3778
Online Access:	Get full text
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