A comparative study of mucilage and pulp polysaccharides from tamarillo fruit (Solanum betaceum Cav.)

A comparative study of mucilage (locular tissue) and pulp polysaccharides from ripe tamarillo fruits (Solanum betaceum Cav.) was carried out. After aqueous and alkaline extractions and various purification steps (freeze–thaw and α-amylase - EC 3.2.1.1 treatments, Fehling precipitation and ultrafiltr...

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Bibliographic Details
Published in:Plant physiology and biochemistry Vol. 104; pp. 278 - 283
Main Authors: do Nascimento, Georgia Erdmann, Iacomini, Marcello, Cordeiro, Lucimara M.C.
Format: Journal Article
Language:English
Published: France Elsevier Masson SAS 01.07.2016
Subjects:
alpha-amylase
Arabinan
Arabinogalactan
arabinogalactans
Carbon-13 Magnetic Resonance Spectroscopy
Chemical Fractionation
Chromatography, Gel
Fruit - metabolism
fruits
Monosaccharides - analysis
Mucilage
nuclear magnetic resonance spectroscopy
Pectins
Pectins - isolation & purification
Plant Mucilage - metabolism
Polysaccharides - isolation & purification
Polysaccharides - metabolism
pulp
Solanum - metabolism
Solanum betaceum
Solubility
sugars
Tamarillo
ultrafiltration
Water - chemistry
Xylan
Solanum betaceum
Xylan
Arabinogalactan
Arabinan
Mucilage
Tamarillo
Pectins
ISSN:0981-9428, 1873-2690, 1873-2690
Online Access:Get full text
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Summary:A comparative study of mucilage (locular tissue) and pulp polysaccharides from ripe tamarillo fruits (Solanum betaceum Cav.) was carried out. After aqueous and alkaline extractions and various purification steps (freeze–thaw and α-amylase - EC 3.2.1.1 treatments, Fehling precipitation and ultrafiltration through 50 kDa cut-off membrane), the obtained fractions from mucilage were analyzed by sugar composition, HPSEC, and NMR spectroscopy analyses. The results showed that the mucilage of tamarillo contains a highly methoxylated homogalacturonans mixed with type I arabinogalactans, a linear (1 → 5)-linked α-L-arabinan, and a linear (1 → 4)-β-D-xylan. A comparison with polysaccharides extracted from the pulp revealed that differences were observed in the yield and in the ratio of extracted polysaccharides. Moreover, structural differences between pulp and mucilage polysaccharides were also observed, such as in the length of side chains of the pectins, and in the degree of branching of the xylans. •Polysaccharides from mucilage of tamarillo fruits were obtained.•Homogalacturonan, arabinogalactan, arabinan, and linear (1 → 4)-β-D-xylan were found•Comparison with those polysaccharides from pulp was performed.•Structural differences between mucilage and pulp polysaccharides were observed.
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ISSN:0981-9428
1873-2690
1873-2690
DOI:10.1016/j.plaphy.2016.04.055