Amplification of human beta-actin gene by the reverse transcriptase-polymerase chain reaction: implications for assessment of RNA from formalin-fixed, paraffin-embedded material

The polymerase chain reaction (PCR) is a powerful method that allows enzymatic amplification of rate target nucleic acid sequences. It has been applied to the amplification of viral genomes from paraffin-embedded pathology specimens. However, interpretation of negative results requires amplification...

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Bibliographic Details
Published in:	The journal of histochemistry and cytochemistry Vol. 44; no. 10; p. 1205
Main Authors:	Dakhama, A, Macek, V, Hogg, J C, Hegele, R G
Format:	Journal Article
Language:	English
Published:	United States 01.10.1996
Subjects:	Actins - genetics Base Composition Base Sequence Biomarkers Blotting, Southern DNA Primers DNA, Neoplasm - analysis DNA, Neoplasm - genetics Formaldehyde Humans Lung Neoplasms - chemistry Lung Neoplasms - genetics Lung Neoplasms - pathology Neoplasm Proteins - genetics Nucleic Acid Denaturation Paraffin Embedding Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - chemistry RNA, Neoplasm - analysis RNA, Neoplasm - chemistry Time Factors Tissue Extracts - chemistry Tissue Fixation
ISSN:	0022-1554
Online Access:	Get more information
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