Characterization of Tunisian pomegranate ( Punica granatum L.) cultivars using amplified fragment length polymorphism analysis

The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power ( R p) co...

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Published in:Scientia horticulturae Vol. 115; no. 3; pp. 231 - 237
Main Authors: Jbir, Rania, Hasnaoui, Néjib, Mars, Messaoud, Marrakchi, Mohamed, Trifi, Mokhtar
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 01.02.2008
Elsevier
Subjects:
AFLP
Agronomy. Soil science and plant productions
amplified fragment length polymorphism
Biological and medical sciences
Cultivars
Fundamental and applied biological sciences. Psychology
genetic markers
genetic polymorphism
genetic variation
Genetics and breeding of economic plants
geographical variation
germplasm
Pomegranate
pomegranates
Punica granatum
Punica granatum L
Tunisia
Tunisia
North Africa
Africa
Punica granatum L
Pomegranate
Tunisia
AFLP
Cultivars
Horticulture
Punica granatum
Medicinal plant
Dicotyledones
Angiospermae
Punicaceae
Spermatophyta
Fruit tree
Amplified fragment length polymorphism marker
Cultivar
ISSN:0304-4238, 1879-1018
Online Access:Get full text
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Summary:The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power ( R p) collective rate value of 129.14 were scored. Data proved that the tested primers were informative to discriminate among cultivars and to survey the genetic diversity in this fruit crop. It has been assumed that the local pomegranate germplasm is characterized by a typically continuous genetic diversity. The derived dendrogram proved that cultivars are clustered independently from their geographical origin and their denomination. In addition, AFLP permitted the generation of a nearly unlimited number of molecular markers that are reliable in differentiating the cultivars and/or the polyclonal varieties.
Bibliography:http://dx.doi.org/10.1016/j.scienta.2007.09.002
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ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2007.09.002