Characterization of Tunisian pomegranate ( Punica granatum L.) cultivars using amplified fragment length polymorphism analysis

The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power ( R p) co...

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Vydáno v:Scientia horticulturae Ročník 115; číslo 3; s. 231 - 237
Hlavní autoři: Jbir, Rania, Hasnaoui, Néjib, Mars, Messaoud, Marrakchi, Mohamed, Trifi, Mokhtar
Médium: Journal Article
Jazyk:angličtina
Vydáno: Amsterdam Elsevier B.V 01.02.2008
Elsevier
Témata:
AFLP
Agronomy. Soil science and plant productions
amplified fragment length polymorphism
Biological and medical sciences
Cultivars
Fundamental and applied biological sciences. Psychology
genetic markers
genetic polymorphism
genetic variation
Genetics and breeding of economic plants
geographical variation
germplasm
Pomegranate
pomegranates
Punica granatum
Punica granatum L
Tunisia
Tunisia
North Africa
Africa
Punica granatum L
Pomegranate
Tunisia
AFLP
Cultivars
Horticulture
Punica granatum
Medicinal plant
Dicotyledones
Angiospermae
Punicaceae
Spermatophyta
Fruit tree
Amplified fragment length polymorphism marker
Cultivar
ISSN:0304-4238, 1879-1018
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Shrnutí:The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power ( R p) collective rate value of 129.14 were scored. Data proved that the tested primers were informative to discriminate among cultivars and to survey the genetic diversity in this fruit crop. It has been assumed that the local pomegranate germplasm is characterized by a typically continuous genetic diversity. The derived dendrogram proved that cultivars are clustered independently from their geographical origin and their denomination. In addition, AFLP permitted the generation of a nearly unlimited number of molecular markers that are reliable in differentiating the cultivars and/or the polyclonal varieties.
Bibliografie:http://dx.doi.org/10.1016/j.scienta.2007.09.002
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2007.09.002