Employing ProteoWizard to Convert Raw Mass Spectrometry Data

After raw data have been captured by mass spectrometers in biological LC-MS/MS experiments, they must be converted from vendor-specific binary files to open-format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format featu...

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Bibliographic Details
Published in:Current protocols in bioinformatics Vol. 46; p. 13.24.1
Main Authors: Holman, Jerry D, Tabb, David L, Mallick, Parag
Format: Journal Article
Language:English
Published: United States 17.06.2014
Subjects:
Databases, Protein
Software
Tandem Mass Spectrometry
User-Computer Interface
LC-MS/MS
database search
proteomics
raw files
mzML
ISSN:1934-340X, 1934-340X
Online Access:Get more information
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Summary:After raw data have been captured by mass spectrometers in biological LC-MS/MS experiments, they must be converted from vendor-specific binary files to open-format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools.
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ISSN:1934-340X
1934-340X
DOI:10.1002/0471250953.bi1324s46