Development of cell-based immunoassays to measure type I collagen in cultured fibroblasts

Excessive deposition of type I collagen by activated fibroblasts is a hallmark of scarring and fibrotic pathologies. Quantitation of collagen I at the protein level is paramount to measure functionally relevant changes during pathological remodeling of the extracellular matrix. We describe two new c...

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Bibliographic Details
Published in:	The international journal of biochemistry & cell biology Vol. 42; no. 11; pp. 1808 - 1815
Main Authors:	Jones, Brian, Bucks, Christine, Wilkinson, Patrick, Pratta, Michael, Farrell, Francis, Sivakumar, Pitchumani
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01.11.2010
Subjects:	Assaying Beta Blotting, Western Cells, Cultured Collagen Collagen Type I - metabolism Collagens Density Enzyme-Linked Immunosorbent Assay Enzymes Extracellular matrix Extracellular Matrix - drug effects Extracellular Matrix - metabolism Fibroblasts Fibroblasts - drug effects Fibroblasts - metabolism Fibrosis Format Human Humans Immunoassay - methods Microscopy, Fluorescence Radioimmunoassay TGFβ Transforming Growth Factor beta - pharmacology NHLF Collagen Fibrosis NHDF TGFβ Fibroblasts Extracellular matrix ICW COL1 ECM ELISA
ISSN:	1357-2725, 1878-5875, 1878-5875
Online Access:	Get full text
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