The relationship between preimplantation morphokinetics of human embryos and sex chromosome pattern

The aim of this study was to determine the relationship between the gender of human embryos and chronological changes during the two pronuclear (2PN) embryonic stage and blastocyst formation on day five after injection using time-lapse imaging and preimplantation genetic testing. A total of 120 coup...

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Published in:Reproductive biology Vol. 18; no. 4; pp. 385 - 389
Main Authors: Zeyad, Ali, Al-Abdulkareem, Bandar, Hammadeh, Mohamad Eid
Format: Journal Article
Language:English
Published: Poland Elsevier B.V 01.12.2018
Subjects:
blastocyst
Embryo cavitation
Embryo preimplantation morphokinetics
Embryo time-lapse
Female
females
Fertilization
genetic testing
humans
image analysis
intracytoplasmic sperm injection
Male
males
sex chromosomes
Sex selection
Fertilization
Embryo time-lapse
Embryo preimplantation morphokinetics
Sex selection
Male
Female
Embryo cavitation
ISSN:1642-431X, 2300-732X, 2300-732X
Online Access:Get full text
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Summary:The aim of this study was to determine the relationship between the gender of human embryos and chronological changes during the two pronuclear (2PN) embryonic stage and blastocyst formation on day five after injection using time-lapse imaging and preimplantation genetic testing. A total of 120 couples who underwent intracytoplasmic sperm injection with preimplantation genetic testing were included in the study. Only normal embryos (n = 416) were enrolled in this study. Time-lapse imaging data of male (n = 227) and female (n = 189) embryo progression was carried out to estimate the times of initial appearance of the 2PN stage, fading times and day five blastulation rates. The results revealed that the 2PN stage (fertilisation) was reached significantly earlier in female embryos (9.09 ± 1.31 h) than in male embryos (9.52 ± 1.48 h, p-value = 0.0044). Conversely, the fading time of 2PN was significantly faster in male embryos (22.13 ± 2.02) than in female embryos (24.16 ± 2.61, p-value < 0.001). The blastulation rate was significantly higher in female than male embryos (68.25% vs. 57.71%; p-value = 0.025).
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ISSN:1642-431X
2300-732X
2300-732X
DOI:10.1016/j.repbio.2018.10.002