Development of Polymeric Nanocomposite (Xyloglucan-co-Methacrylic Acid/Hydroxyapatite/SiO2) Scaffold for Bone Tissue Engineering Applications—In-Vitro Antibacterial, Cytotoxicity and Cell Culture Evaluation

Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, i...

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Published in:Polymers Vol. 12; no. 6; p. 1238
Main Authors: Aslam Khan, Muhammad Umar, Mehboob, Hassan, Abd Razak, Saiful Izwan, Yahya, Mohd Yazid, Mohd Yusof, Abdul Halim, Ramlee, Muhammad Hanif, Sahaya Anand, T. Joseph, Hassan, Rozita, Aziz, Athar, Amin, Rashid
Format: Journal Article
Language:English
Published: Basel MDPI AG 29.05.2020
MDPI
Subjects:
Biocompatibility
Biodegradability
Biological activity
Biomechanics
Biomedical materials
Biopolymers
Bones
Cell culture
Composite materials
Crystal defects
Fourier transforms
Free radical polymerization
Free radicals
Functional groups
Hydroxyapatite
Infrared analysis
Mechanical properties
Methacrylic acid
Morphology
Nanocomposites
Nanoparticles
Particle size
Polymerization
Polysaccharides
Porosity
Regeneration (physiology)
Scaffolds
Scanning electron microscopy
Silicon dioxide
Spectrum analysis
Studies
Tissue engineering
Toxicity
Japan
ISSN:2073-4360, 2073-4360
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Summary:Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone.
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ISSN:2073-4360
2073-4360
DOI:10.3390/polym12061238