Quantification of the sensitivity increase of a micro-high-performance liquid chromatography–electrospray ionization mass spectrometry system with decreasing column diameter
This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compar...
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| Vydáno v: | Journal of Chromatography A Ročník 976; číslo 1; s. 11 - 18 |
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| Hlavní autoři: | , , |
| Médium: | Journal Article Konferenční příspěvek |
| Jazyk: | angličtina |
| Vydáno: |
Amsterdam
Elsevier B.V
08.11.2002
Elsevier |
| Témata: | |
| ISSN: | 0021-9673 |
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| Abstract | This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from −20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%. |
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| AbstractList | This study details the sensitivity achieved with capillary columns when used with a micro-HPLC-electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from -20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%.This study details the sensitivity achieved with capillary columns when used with a micro-HPLC-electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from -20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%. This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from −20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%. This study details the sensitivity achieved with capillary columns when used with a micro-HPLC-electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from -20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%. |
| Author | Smith, N.W Mallet, D Legido-Quigley, C |
| Author_xml | – sequence: 1 givenname: C surname: Legido-Quigley fullname: Legido-Quigley, C email: c.legidoquigley@ic.ac.uk organization: Centre for Analytical Sciences, RSC1, Imperial College London, Exhibition Road, London SW7, UK – sequence: 2 givenname: N.W surname: Smith fullname: Smith, N.W organization: Centre for Analytical Sciences, RSC1, Imperial College London, Exhibition Road, London SW7, UK – sequence: 3 givenname: D surname: Mallet fullname: Mallet, D organization: CEDD DMPK, GlaxoSmithKline R&D, Park Road, Ware, Herts SG1 0DP, UK |
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| Cites_doi | 10.1016/S0021-9673(00)93823-8 10.1016/0021-9673(75)85032-1 10.1016/S0021-9673(00)85595-8 10.1016/S0021-9673(01)01570-9 10.1016/S0021-9673(96)00617-6 10.1016/S0021-9673(00)95268-3 10.1080/10826079508009220 |
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| Keywords | Sensitivity Liquid chromatography Column diameter Capillary column Corticosteroid Coupled method Bronchodilator Antiinflammatory agent Salmeterol HPLC chromatography β-Adrenergic receptor agonist Fluticasone Antiasthma agent Experimental study Electrospray Microbore column Reversed phase chromatography Mass spectrometry Diameter |
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| References | Scott, Kucera (BIB3) 1979; 186 Coufal, Čihák, Suchánková, Tesařová, Bosáková, Štulı́k (BIB8) 2002; 946 Braithwaite, Smith (BIB9) 1996 Scott (BIB5) 1980; 15 Scott, Kucera (BIB4) 1979; 184 Scott, Kucera (BIB2) 1979; 169 Ryan (BIB6) 1995; 18 Scott, Kucera (BIB1) 1976; 125 Zhou, Hamburger (BIB10) 1996; 755 Dear, Mallet, Plumb (BIB7) 2001; 14 Zhou (10.1016/S0021-9673(02)01156-1_BIB10) 1996; 755 Scott (10.1016/S0021-9673(02)01156-1_BIB3) 1979; 186 Coufal (10.1016/S0021-9673(02)01156-1_BIB8) 2002; 946 Braithwaite (10.1016/S0021-9673(02)01156-1_BIB9) 1996 Scott (10.1016/S0021-9673(02)01156-1_BIB2) 1979; 169 Scott (10.1016/S0021-9673(02)01156-1_BIB5) 1980; 15 Scott (10.1016/S0021-9673(02)01156-1_BIB1) 1976; 125 Scott (10.1016/S0021-9673(02)01156-1_BIB4) 1979; 184 Dear (10.1016/S0021-9673(02)01156-1_BIB7) 2001; 14 Ryan (10.1016/S0021-9673(02)01156-1_BIB6) 1995; 18 |
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| Snippet | This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two... This study details the sensitivity achieved with capillary columns when used with a micro-HPLC-electrospray ionization MS system. It is comprised of two... |
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| SubjectTerms | Analysis Analytical chemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Exact sciences and technology General pharmacology Medical sciences Other chromatographic methods Pharmacology. Drug treatments Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Spectrophotometry, Ultraviolet |
| Title | Quantification of the sensitivity increase of a micro-high-performance liquid chromatography–electrospray ionization mass spectrometry system with decreasing column diameter |
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