High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1

Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatograp...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Vol. 729; no. 1; pp. 369 - 374
Main Authors: Arcelloni, Cinzia, Aiuti, Alessandro, Cipponi, Arcadi, Paroni, Rita
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 11.06.1999
Subjects:
Animals
Cell Line
Chemokine CXCL12
Chemokines
Chemokines, CXC - isolation & purification
Chemotaxis, Leukocyte
Chromatography, High Pressure Liquid - methods
Electrophoresis, Capillary - methods
Humans
Mice
SDF-1α
SDF-1α
Chemokines
ISSN:0378-4347, 1387-2273
Online Access:Get full text
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Summary:Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1α from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1α from chemical synthesis and to resolve murine and human SDF-1α, differing by only one amino acid. Chemotactic activity of the murine SDF-1α was tested on the basis of CE quantification.
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ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(99)00154-1