High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1
Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatograp...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatography. B, Biomedical sciences and applications Jg. 729; H. 1; S. 369 - 374 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
Netherlands
Elsevier B.V
11.06.1999
|
| Schlagworte: | |
| ISSN: | 0378-4347, 1387-2273 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1α from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1α from chemical synthesis and to resolve murine and human SDF-1α, differing by only one amino acid. Chemotactic activity of the murine SDF-1α was tested on the basis of CE quantification. |
|---|---|
| AbstractList | Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1alpha) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1alpha from chemical synthesis and to resolve murine and human SDF-1alpha, differing by only one amino acid. Chemotactic activity of the murine SDF-1alpha was tested on the basis of CE quantification.Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1alpha) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1alpha from chemical synthesis and to resolve murine and human SDF-1alpha, differing by only one amino acid. Chemotactic activity of the murine SDF-1alpha was tested on the basis of CE quantification. Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1α from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1α from chemical synthesis and to resolve murine and human SDF-1α, differing by only one amino acid. Chemotactic activity of the murine SDF-1α was tested on the basis of CE quantification. Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1alpha) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1alpha from chemical synthesis and to resolve murine and human SDF-1alpha, differing by only one amino acid. Chemotactic activity of the murine SDF-1alpha was tested on the basis of CE quantification. |
| Author | Aiuti, Alessandro Cipponi, Arcadi Arcelloni, Cinzia Paroni, Rita |
| Author_xml | – sequence: 1 givenname: Cinzia surname: Arcelloni fullname: Arcelloni, Cinzia email: arcelloni.cinzia@hsr.it organization: Laboratory of Separative Techniques, Dept. of Laboratory Medicine, IRCCS, H S. Raffaele, Via Olgettina 60, 20132 Milan, Italy – sequence: 2 givenname: Alessandro surname: Aiuti fullname: Aiuti, Alessandro organization: Telethon Institute for Gene Therapy, DIBIT, Via Olgettina 58, 20132 Milan, Italy – sequence: 3 givenname: Arcadi surname: Cipponi fullname: Cipponi, Arcadi organization: Telethon Institute for Gene Therapy, DIBIT, Via Olgettina 58, 20132 Milan, Italy – sequence: 4 givenname: Rita surname: Paroni fullname: Paroni, Rita organization: Laboratory of Separative Techniques, Dept. of Laboratory Medicine, IRCCS, H S. Raffaele, Via Olgettina 60, 20132 Milan, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10410964$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkctu1TAQhr0oojceAeQVgkWofezkxGKBUAUUqRKLlrVlO-NmIIlzbKcSb8Ej45xTLmLTlaXR9_8az3dKjqYwASHPOXvDGW8ubpjYtpUUcvtKqdeM8VpW_Iic_Bkfk9OUvjEmJNtsnpJjziRnqpEn5OcV3vXVDNGHOJrJAR1wt2BHXR_DaHK4i2bu0dF5iejRmYxhomYqgJlxGEz8QWEAl2OY-xAhYaK7xUz5Lxw8zT2UQhjDd5yAprxWD9TBMFQdRLyHjnrjcogVPydPvBkSPHt4z8jXjx9uL6-q6y-fPl--v66cUCxXtu2Y9I2ThoOxvjHMtt6KeitapgoiuXSNadsGhLWMqbpWltkt517WTgouzsjLQ-8cw26BlPWIad3ITBCWpBvVKibEpoAvHsDFjtDpOeJYfq1_37AA9QFwMaQUwf-D6NWP3vvRqwitlN770esGb__LOcz7k-VocHg0_e6QhnKke4Sok0MoAjuMRYfuAj7S8AsvmK8t |
| CitedBy_id | crossref_primary_10_1182_blood_V95_3_769_003a49_769_775 crossref_primary_10_1007_s00216_005_3120_9 crossref_primary_10_1002_1522_2683_200111_22_19_4163__AID_ELPS4163_3_0_CO_2_9 |
| Cites_doi | 10.1126/science.8342023 10.1073/pnas.91.6.2305 10.1038/382829a0 10.1084/jem.184.3.1101 10.1021/ac00085a033 10.1006/abio.1997.2553 10.1084/jem.185.1.111 10.1021/bi00226a021 10.1016/S0065-2776(08)60509-X 10.1016/S0076-6879(97)87018-8 10.1146/annurev.ph.57.030195.004143 10.1038/382833a0 |
| ContentType | Journal Article |
| Copyright | 1999 Elsevier Science B.V. |
| Copyright_xml | – notice: 1999 Elsevier Science B.V. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/S0378-4347(99)00154-1 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry |
| EndPage | 374 |
| ExternalDocumentID | 10410964 10_1016_S0378_4347_99_00154_1 S0378434799001541 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: Telethon grantid: TGT06S01 |
| GroupedDBID | --K --M .HR .~1 0R~ 1RT 1~5 3O- 4.4 4G. 5GY 5RE 5VS 7-5 71M 8P~ AABNK AACTN AAEDT AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABEFU ABJNI ABMAC ABXDB ACDAQ ACNNM ACRLP ADMUD AFTJW AFXIZ AGHFR AGRDE AGUBO AHHHB AIEXJ AIKHN AITUG AJQLL ALMA_UNASSIGNED_HOLDINGS AMRAJ AXJTR AZFZN BLXMC CS3 EBS EFJIC EJD EO8 EO9 EP2 EP3 FDB FGOYB FIRID G-2 G-Q G8K HLW HMU HZ~ H~9 IHE L7B LX3 M36 M41 MO0 O-L O9- OAUVE OZT Q38 R2- RIG ROL SBG SCB SCC SCH SDF SDG SPC SSK SSU SSZ T5K WUQ XFK XPP Y6R ZGI 9DU AAYWO AAYXX ABDPE ABWVN ACLOT ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFPUW AGQPQ AIGII AKBMS AKRWK AKYEP ANKPU CITATION EFKBS ~HD 1B1 1~. 29K 53G AATTM AAXKI ABUDA ACGFS ADBBV ADEZE AEJYF AEKER AFZHZ AGYEJ AI. AIIUN BKOJK CGR CUY CVF D-I ECM EIF F5P FLBIZ FNPLU FYGXN KOM NPM NQ- P-9 PC. RNS RPZ VH1 WH7 7X8 |
| ID | FETCH-LOGICAL-c390t-b8d04f6c4a1eabf6a0b8fb3573809c39414c6a886e3bb009559b0b711f45c4313 |
| ISICitedReferencesCount | 3 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000080932900043&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0378-4347 1387-2273 |
| IngestDate | Fri Jul 11 00:04:59 EDT 2025 Fri May 30 10:48:42 EDT 2025 Sat Nov 29 03:33:20 EST 2025 Tue Nov 18 21:25:39 EST 2025 Fri Feb 23 02:17:52 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | false |
| Issue | 1 |
| Keywords | SDF-1α Chemokines |
| Language | English |
| License | https://www.elsevier.com/tdm/userlicense/1.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c390t-b8d04f6c4a1eabf6a0b8fb3573809c39414c6a886e3bb009559b0b711f45c4313 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 10410964 |
| PQID | 69890332 |
| PQPubID | 23479 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_69890332 pubmed_primary_10410964 crossref_primary_10_1016_S0378_4347_99_00154_1 crossref_citationtrail_10_1016_S0378_4347_99_00154_1 elsevier_sciencedirect_doi_10_1016_S0378_4347_99_00154_1 |
| PublicationCentury | 1900 |
| PublicationDate | 1999-06-11 |
| PublicationDateYYYYMMDD | 1999-06-11 |
| PublicationDate_xml | – month: 06 year: 1999 text: 1999-06-11 day: 11 |
| PublicationDecade | 1990 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Journal of chromatography. B, Biomedical sciences and applications |
| PublicationTitleAlternate | J Chromatogr B Biomed Sci Appl |
| PublicationYear | 1999 |
| Publisher | Elsevier B.V |
| Publisher_xml | – name: Elsevier B.V |
| References | Baggiolini, Dewal, Moser (BIB1) 1994; 55 Tashiro, Tada, Heiker, Shirozu, Nakano, Honjo (BIB3) 1993; 261 Oberlin, Amara, Bachelerie, Bessia, Virelizier, Arenzana-Seisdedos, Schwarts, Heard, Clark-Lewis, Legler, Loetscher, Baggiolini, Moser (BIB8) 1996; 382 Roche, Anderson, Oda, Riggs, Strausbauch, Okazaki, Wettestein, Landers (BIB12) 1998; 258 Bleul, Fuhlbrigge, Casasnovas, Aiuti, Springer (BIB5) 1996; 184 Itoh, Tezuka, Sakoda, Konno, Nagata, Uchiyama, Uchino, Mori (BIB9) 1989; 17 Clark-Lewis, Moser, Walz, Baggiolini, Scott, Aebersold (BIB10) 1991; 30 Nashabeh, Greve, Kirby, Foret, Karger, Relfsnyder, Bullder (BIB13) 1994; 66 Aiuti, Webb, Bleul, Springer, Gutierrez-Ramos (BIB6) 1997; 185 Bleul, Farzan, Choe, Parolin, Clark-Lewis, Sodroski, Springer (BIB7) 1996; 382 Nagasawa, Kikutani, Kishimoto (BIB4) 1994; 91 Clark-Lewis, Vo, Owen, Anderson (BIB11) 1997; 287 Springer (BIB2) 1995; 57 Clark-Lewis (10.1016/S0378-4347(99)00154-1_BIB10) 1991; 30 Aiuti (10.1016/S0378-4347(99)00154-1_BIB6) 1997; 185 Oberlin (10.1016/S0378-4347(99)00154-1_BIB8) 1996; 382 Roche (10.1016/S0378-4347(99)00154-1_BIB12) 1998; 258 Clark-Lewis (10.1016/S0378-4347(99)00154-1_BIB11) 1997; 287 Nagasawa (10.1016/S0378-4347(99)00154-1_BIB4) 1994; 91 Baggiolini (10.1016/S0378-4347(99)00154-1_BIB1) 1994; 55 Tashiro (10.1016/S0378-4347(99)00154-1_BIB3) 1993; 261 Nashabeh (10.1016/S0378-4347(99)00154-1_BIB13) 1994; 66 Bleul (10.1016/S0378-4347(99)00154-1_BIB7) 1996; 382 Springer (10.1016/S0378-4347(99)00154-1_BIB2) 1995; 57 Bleul (10.1016/S0378-4347(99)00154-1_BIB5) 1996; 184 Itoh (10.1016/S0378-4347(99)00154-1_BIB9) 1989; 17 |
| References_xml | – volume: 17 start-page: 145 year: 1989 ident: BIB9 publication-title: Exp. Hematol. – volume: 258 start-page: 87 year: 1998 ident: BIB12 publication-title: Anal. Biochem. – volume: 185 start-page: 111 year: 1997 ident: BIB6 publication-title: J. Exp. Med. – volume: 287 start-page: 233 year: 1997 ident: BIB11 publication-title: Methods Enzymol. – volume: 57 start-page: 827 year: 1995 ident: BIB2 publication-title: Annu. Rev. Physiol. – volume: 30 start-page: 3128 year: 1991 ident: BIB10 publication-title: Biochemistry – volume: 66 start-page: 2148 year: 1994 ident: BIB13 publication-title: Anal. Chem. – volume: 91 start-page: 2305 year: 1994 ident: BIB4 publication-title: Proc. Nat. Acad. Sci. USA – volume: 261 start-page: 600 year: 1993 ident: BIB3 publication-title: Science – volume: 382 start-page: 829 year: 1996 ident: BIB7 publication-title: Nature – volume: 382 start-page: 833 year: 1996 ident: BIB8 publication-title: Nature – volume: 55 start-page: 97 year: 1994 ident: BIB1 publication-title: Adv. Immunol. – volume: 184 start-page: 1101 year: 1996 ident: BIB5 publication-title: J. Exp. Med – volume: 261 start-page: 600 year: 1993 ident: 10.1016/S0378-4347(99)00154-1_BIB3 publication-title: Science doi: 10.1126/science.8342023 – volume: 91 start-page: 2305 year: 1994 ident: 10.1016/S0378-4347(99)00154-1_BIB4 publication-title: Proc. Nat. Acad. Sci. USA doi: 10.1073/pnas.91.6.2305 – volume: 382 start-page: 829 year: 1996 ident: 10.1016/S0378-4347(99)00154-1_BIB7 publication-title: Nature doi: 10.1038/382829a0 – volume: 184 start-page: 1101 year: 1996 ident: 10.1016/S0378-4347(99)00154-1_BIB5 publication-title: J. Exp. Med. doi: 10.1084/jem.184.3.1101 – volume: 17 start-page: 145 year: 1989 ident: 10.1016/S0378-4347(99)00154-1_BIB9 publication-title: Exp. Hematol. – volume: 66 start-page: 2148 year: 1994 ident: 10.1016/S0378-4347(99)00154-1_BIB13 publication-title: Anal. Chem. doi: 10.1021/ac00085a033 – volume: 258 start-page: 87 year: 1998 ident: 10.1016/S0378-4347(99)00154-1_BIB12 publication-title: Anal. Biochem. doi: 10.1006/abio.1997.2553 – volume: 185 start-page: 111 year: 1997 ident: 10.1016/S0378-4347(99)00154-1_BIB6 publication-title: J. Exp. Med. doi: 10.1084/jem.185.1.111 – volume: 30 start-page: 3128 year: 1991 ident: 10.1016/S0378-4347(99)00154-1_BIB10 publication-title: Biochemistry doi: 10.1021/bi00226a021 – volume: 55 start-page: 97 year: 1994 ident: 10.1016/S0378-4347(99)00154-1_BIB1 publication-title: Adv. Immunol. doi: 10.1016/S0065-2776(08)60509-X – volume: 287 start-page: 233 year: 1997 ident: 10.1016/S0378-4347(99)00154-1_BIB11 publication-title: Methods Enzymol. doi: 10.1016/S0076-6879(97)87018-8 – volume: 57 start-page: 827 year: 1995 ident: 10.1016/S0378-4347(99)00154-1_BIB2 publication-title: Annu. Rev. Physiol. doi: 10.1146/annurev.ph.57.030195.004143 – volume: 382 start-page: 833 year: 1996 ident: 10.1016/S0378-4347(99)00154-1_BIB8 publication-title: Nature doi: 10.1038/382833a0 |
| SSID | ssj0034022 ssj0034021 |
| Score | 1.2788074 |
| Snippet | Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC–chemokine stromal cell derived factor-1... Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 369 |
| SubjectTerms | Animals Cell Line Chemokine CXCL12 Chemokines Chemokines, CXC - isolation & purification Chemotaxis, Leukocyte Chromatography, High Pressure Liquid - methods Electrophoresis, Capillary - methods Humans Mice SDF-1α |
| Title | High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1 |
| URI | https://dx.doi.org/10.1016/S0378-4347(99)00154-1 https://www.ncbi.nlm.nih.gov/pubmed/10410964 https://www.proquest.com/docview/69890332 |
| Volume | 729 |
| WOSCitedRecordID | wos000080932900043&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 issn: 0378-4347 databaseCode: AIEXJ dateStart: 19950106 customDbUrl: isFulltext: true dateEnd: 20011225 titleUrlDefault: https://www.sciencedirect.com omitProxy: false ssIdentifier: ssj0034022 providerName: Elsevier |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLZKhwQvCDYY5TL8wBCoykjitLEfq2oIkKgmGFLfIttJNIs2Cb1p41fwW_kFHMdO0haVsQdeotaqT9ycL_axz-VD6KWAZavH_dSJYbl0gsTjDpMkgfcKfu56aUqTuCSbCEcjOh6zs1brV5ULs5qEWUYvL1nxX1UNbaBsnTp7A3XXQqEBPoPS4Qpqh-s_KV5HbjjFWj7ARH1fqrgrL2Y5mKemRLWS3WI502FCBgBldhsvNAWRzs413DjFRQ6bcTXXmZcmpqi2L7W5Cuqe5t-0lTpfaNGTrvYCODH8yRWYsYbJx_F2mL8bw7k60aSYU-s0WneqN4DUwvOSgKo7VNkPVa8nA7U0MQmDieZz0RUYas-KKgrbBwTwWDUes5lt_6wWvDn6sOUSHDs125Qv2AIHxJTsrKbz0J6gqI3kSjM9E0MLY1d6YviB_lhEzHnGl1r6sd6UHvustDftY9so3L21oNZhjk0EHYiKtKiIsagUE8GOfc8Pe4y20d7gw-n4Y2U_ENjRG9-XvX2Td_a2GdNrxt7Y8eyyqHbtmErL6fw-umd1jgcGqg9QK8n20cEgA-VPr_ArXAYhl96dfXRnWBEQHqCf20jGBsl4C8l4HckY9I9rJOMtJONNJOM8xYBkXCMZWyTjdSTjCskP0dd3p-fD944lEHEkYe7CETR2g7QvA-4lXKR97gqaCtILCXVhRmKBF8g-p7SfECFMMUbhitDz0qAnwbImj1A7y7PkMcLU60nJJGceFYFLhQiZp4MpYj9JeM-XHRRUSoikra6vSV4m0V9B0EEndbfClJe5rgOtNBxZG9nYvhGg97quLypERKBJ_SB5luTLeaRJZF1C_A46NEBZG0vguawfPLnpOJ-iu80b-wy1F7Nl8hzdlquFms-O0K1wTI8s8OHb6OzTb9Ux9w0 |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=High-performance+liquid+chromatographic+purification+and+capillary+electrophoresis+quantification+of+the+chemokine+stromal+cell-derived+factor-1&rft.jtitle=Journal+of+chromatography.+Biomedical+applications&rft.au=Arcelloni%2C+Cinzia&rft.au=Aiuti%2C+Alessandro&rft.au=Cipponi%2C+Arcadi&rft.au=Paroni%2C+Rita&rft.date=1999-06-11&rft.issn=0378-4347&rft.volume=729&rft.issue=1-2&rft.spage=369&rft.epage=374&rft_id=info:doi/10.1016%2FS0378-4347%2899%2900154-1&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_S0378_4347_99_00154_1 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0378-4347&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0378-4347&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0378-4347&client=summon |