Messenger RNA differential display reverse‐transcriptase‐polymerase‐chain‐reaction analysis of a progestogen‐suppressive gene in a human endometrial‐cancer cell line

Progestogen suppresses the progression of endometrial cancer and has an important effect on the secretory change of human endometrium. We characterized the progestogen‐induced alterations of gene expression in a human endometrial‐cancer cell line using a mRNA differential‐display reverse‐transcripta...

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Bibliographic Details
Published in:International journal of cancer Vol. 78; no. 1; pp. 125 - 129
Main Authors: Sakata, Masahiro, Kurachi, Hirohisa, Morishige, Ken‐ichirou, Ogura, Kazuhiro, Yamaguchi, Masaaki, Nishio, Yukihiro, Ikegami, Hiromasa, Miyake, Akira, Murata, Yuji
Format: Journal Article
Language:English
Published: New York Wiley Subscription Services, Inc., A Wiley Company 25.09.1998
Wiley-Liss
Subjects:
Antineoplastic Agents, Hormonal - pharmacology
Biological and medical sciences
Blotting, Northern
Endometrial Neoplasms - genetics
Enoyl-CoA Hydratase - genetics
Female
Female genital diseases
General aspects (metabolism, cell proliferation, established cell line...)
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Medroxyprogesterone Acetate - pharmacology
Neoplasm Proteins - genetics
Polymerase Chain Reaction - methods
Progestins - genetics
RNA, Messenger - analysis
RNA, Neoplasm - analysis
Tumor cell
Tumor Cells, Cultured
Tumors
Human
Cell proliferation
Cell culture
Malignant tumor
Experimental study
Gene expression
Female genital diseases
Polymerase chain reaction
Ovarian diseases
Ovary
Messenger RNA
Female
Progesterone
ISSN:0020-7136, 1097-0215
Online Access:Get full text
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Summary:Progestogen suppresses the progression of endometrial cancer and has an important effect on the secretory change of human endometrium. We characterized the progestogen‐induced alterations of gene expression in a human endometrial‐cancer cell line using a mRNA differential‐display reverse‐transcriptase‐polymerase‐chain‐reaction (DDRT‐PCR) method. After 5‐day incubation of Ishikawa endometrial‐cancer cells, with or without 100 nM medroxyprogesterone acetate (MPA), total RNA was isolated from confluent cells. We identified 8 candidate genes by mRNA differential display by screening up to approximately 3,000 mRNA species. Among these, 2 genes named T21A and T21B showed a decrease in mRNA by MPA treatment when analyzed by Northern blot. Nucleotide sequence showed that clone T21A was part of human mitochondrial short‐chain enoyl‐CoA hydratase cDNA. The other clone, T21B, showed no homology with any known nucleotide sequences. Northern‐blot analysis using T21A and T21B clones as probes showed a decrease in mRNA in human endometrium from the luteal stage, with high serum estradiol and progesterone levels, as compared with that from the early follicular stage, with low serum estradiol and progesterone levels, and that from the pre‐ovulatory stage with high serum estradiol and low progesterone levels. These findings suggest that mRNA DDRT‐PCR could be used to identify the candidate genes regulated by progestogen in human endometrial cancer and in normal human endometrium. Int. J. Cancer 78:125–129, 1998.© 1998 Wiley‐Liss, Inc.
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ISSN:0020-7136
1097-0215
DOI:10.1002/(SICI)1097-0215(19980925)78:1<125::AID-IJC20>3.0.CO;2-9