Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase

The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested ooc...

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Vydáno v:Development, growth & differentiation Ročník 58; číslo 3; s. 250 - 259
Hlavní autoři: Stricker, Stephen A., Beckstrom, Bradley, Mendoza, Cristina, Stanislawski, Emma, Wodajo, Tewodros
Médium: Journal Article
Jazyk:angličtina
Vydáno: Japan Wiley Subscription Services, Inc 01.04.2016
Témata:
Animals
apoptosis
Apoptosis - physiology
Butadienes - pharmacology
Cells, Cultured
Cellular Senescence - drug effects
Cellular Senescence - physiology
Cerebratulus
Colforsin - pharmacology
Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases - metabolism
Female
Immunoblotting
Invertebrates - cytology
Invertebrates - drug effects
Invertebrates - metabolism
Kinases
Maturation-Promoting Factor - antagonists & inhibitors
Maturation-Promoting Factor - metabolism
necroptosis
nemertean
Nitriles - pharmacology
non‐apoptotic regulated cell death
Oocytes - cytology
Oocytes - drug effects
Oocytes - physiology
Protein Kinase Inhibitors - pharmacology
Purines - pharmacology
Seawater - parasitology
Time Factors
Vasodilator Agents
apoptosis
nemertean
Cerebratulus
non-apoptotic regulated cell death
necroptosis
ISSN:0012-1592, 1440-169X
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Shrnutí:The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho‐specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation‐initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes. To supplement studies of other oocytes, morphological patterns of extraovarian aging are described for metaphase‐I‐arrested oocytes produced by Cerebratulus, an externally fertilizing marine worm of the phylum Nemertea. In addition, pharmacological modulators are combined with immunoblotting assays to provide data for this protostome that can be compared with previous reports for echinoderm and vertebrate deuterostomes.
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ISSN:0012-1592
1440-169X
DOI:10.1111/dgd.12269