DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid‐Based Cytology

ABSTRACT Background DNA flow cytometry using LC‐1000 is utilized for analyzing cellular and nuclear lengths and DNA content in individual cells with samples in cell preservation solutions to acquire the cell proliferation index (CPIx). This study aimed to evaluate the performance of body cavity flui...

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Vydané v:Diagnostic cytopathology Ročník 53; číslo 5; s. 227 - 237
Hlavní autori: Ryu, Ayumi, Honma, Keiichiro, Tsuzaki, Sayoko, Yoshioka, Risa, Tanada, Satoshi, Yamamoto, Takashi, Satomi, Hidetoshi, Nagata, Shigenori, Yamasaki, Tomoyuki, Ohue, Masayuki
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Hoboken, USA John Wiley & Sons, Inc 01.05.2025
Wiley Subscription Services, Inc
Predmet:
Adult
Aged
Aged, 80 and over
body cavity fluids
Body Fluids - cytology
Cellular biology
Cytodiagnosis - methods
Deoxyribonucleic acid
DNA
DNA - analysis
Female
Flow cytometry
Flow Cytometry - methods
Humans
liquid‐based cytology
Male
Middle Aged
Neoplasms - diagnosis
Neoplasms - pathology
body cavity fluids
flow cytometry
DNA
liquid‐based cytology
ISSN:8755-1039, 1097-0339, 1097-0339
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Shrnutí:ABSTRACT Background DNA flow cytometry using LC‐1000 is utilized for analyzing cellular and nuclear lengths and DNA content in individual cells with samples in cell preservation solutions to acquire the cell proliferation index (CPIx). This study aimed to evaluate the performance of body cavity fluid cytology using LC‐1000. As cell preservation solutions, liquid‐based cytology (LBC) by Cellprep (CP) was compared with CelVerse (CeV), specifically designed for the LC‐1000. Methods Overall, we evaluated 43 samples (20 malignant and 23 benign) of body cavity fluids suspended in CeV and 59 samples (28 malignant and 31 benign) suspended in CP. The CPIx was compared between malignant and benign cases of CeV and CP. Further, the subject groups were divided according to the SubG1% in a sample. Results The CPIx in CeV was 0.14–10.22 (median 0.62) in malignant cases and 0.09–4.64 (median 0.19) in benign cases (p < 0.001); additionally, CP displayed 0.14–9.87 (median 0.84) in malignant cases and 0.11–3.02 (median 0.22) in benign cases (p < 0.001). The area under the curve (AUC) was 0.811 for CeV and 0.776 for CP. The difference in SubG1% between the CeV and CP groups was statistically significant (p < 0.0001) and influenced the AUC in CP. Conclusion The CPIx in CeV or CP samples using the LC‐1000 may be applicable as an objective indicator in body cavity fluid cytology. Samples preserved in CeV provided more consistent results, unaffected by SubG1%, when compared to those stored in CP.
Bibliografia:The authors received no specific funding for this work.
Funding
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ISSN:8755-1039
1097-0339
1097-0339
DOI:10.1002/dc.25451