CircSNCA downregulation by pramipexole treatment mediates cell apoptosis and autophagy in Parkinson's disease by targeting miR-7
We aimed to explore the mechanism of pramipexole (PPX) actions in the treatment of Parkinson's disease (PD). Genes related to PD and PPX were screened through bioinformatics retrieval. The PD model was constructed by applying 1-methyl-4-phenylpyridinium (MMP+). The RNA expression levels of circ...
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| Vydáno v: | Aging (Albany, NY.) Ročník 10; číslo 6; s. 1281 |
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| Hlavní autoři: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
28.06.2018
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| Témata: | |
| ISSN: | 1945-4589, 1945-4589 |
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| Abstract | We aimed to explore the mechanism of pramipexole (PPX) actions in the treatment of Parkinson's disease (PD). Genes related to PD and PPX were screened through bioinformatics retrieval. The PD model was constructed by applying 1-methyl-4-phenylpyridinium (MMP+). The RNA expression levels of circSNCA,
, apoptosis-related genes (
,
,
,
and
) and miR-7 were detected by qRT-PCR. Protein expression was determined by western blot. The interactions between circSNCA-miR-7-
were verified by dual luciferase assay and immunofluorescence localization. Cell viability was determined by MTT assay.
and circSNCA expression levels in PD were downregulated after PPX treatment, consistent with the levels of pro-apoptotic genes. CircSNCA increased
expression by downregulating miR-7 in PD as a competitive endogenous RNA (ceRNA). Lower circSNCA expression was associated with the reduced expression of pro-apoptotic (
,
,
and
) proteins. CircSNCA downregulation could decrease apoptosis and induce autophagy in PD. In conclusion, the downregulation of circSNCA by PPX treatment reduced cell apoptosis and promoted cell autophagy in PD via a mechanism that served as a miR-7 sponge to upregulate
. |
|---|---|
| AbstractList | We aimed to explore the mechanism of pramipexole (PPX) actions in the treatment of Parkinson's disease (PD). Genes related to PD and PPX were screened through bioinformatics retrieval. The PD model was constructed by applying 1-methyl-4-phenylpyridinium (MMP+). The RNA expression levels of circSNCA,
, apoptosis-related genes (
,
,
,
and
) and miR-7 were detected by qRT-PCR. Protein expression was determined by western blot. The interactions between circSNCA-miR-7-
were verified by dual luciferase assay and immunofluorescence localization. Cell viability was determined by MTT assay.
and circSNCA expression levels in PD were downregulated after PPX treatment, consistent with the levels of pro-apoptotic genes. CircSNCA increased
expression by downregulating miR-7 in PD as a competitive endogenous RNA (ceRNA). Lower circSNCA expression was associated with the reduced expression of pro-apoptotic (
,
,
and
) proteins. CircSNCA downregulation could decrease apoptosis and induce autophagy in PD. In conclusion, the downregulation of circSNCA by PPX treatment reduced cell apoptosis and promoted cell autophagy in PD via a mechanism that served as a miR-7 sponge to upregulate
. We aimed to explore the mechanism of pramipexole (PPX) actions in the treatment of Parkinson's disease (PD). Genes related to PD and PPX were screened through bioinformatics retrieval. The PD model was constructed by applying 1-methyl-4-phenylpyridinium (MMP+). The RNA expression levels of circSNCA, SNCA, apoptosis-related genes (BCL2, CASP3, BAX, PTEN and P53) and miR-7 were detected by qRT-PCR. Protein expression was determined by western blot. The interactions between circSNCA-miR-7-SNCA were verified by dual luciferase assay and immunofluorescence localization. Cell viability was determined by MTT assay. SNCA and circSNCA expression levels in PD were downregulated after PPX treatment, consistent with the levels of pro-apoptotic genes. CircSNCA increased SNCA expression by downregulating miR-7 in PD as a competitive endogenous RNA (ceRNA). Lower circSNCA expression was associated with the reduced expression of pro-apoptotic (CASP3, BAX, PTEN and P53) proteins. CircSNCA downregulation could decrease apoptosis and induce autophagy in PD. In conclusion, the downregulation of circSNCA by PPX treatment reduced cell apoptosis and promoted cell autophagy in PD via a mechanism that served as a miR-7 sponge to upregulate SNCA.We aimed to explore the mechanism of pramipexole (PPX) actions in the treatment of Parkinson's disease (PD). Genes related to PD and PPX were screened through bioinformatics retrieval. The PD model was constructed by applying 1-methyl-4-phenylpyridinium (MMP+). The RNA expression levels of circSNCA, SNCA, apoptosis-related genes (BCL2, CASP3, BAX, PTEN and P53) and miR-7 were detected by qRT-PCR. Protein expression was determined by western blot. The interactions between circSNCA-miR-7-SNCA were verified by dual luciferase assay and immunofluorescence localization. Cell viability was determined by MTT assay. SNCA and circSNCA expression levels in PD were downregulated after PPX treatment, consistent with the levels of pro-apoptotic genes. CircSNCA increased SNCA expression by downregulating miR-7 in PD as a competitive endogenous RNA (ceRNA). Lower circSNCA expression was associated with the reduced expression of pro-apoptotic (CASP3, BAX, PTEN and P53) proteins. CircSNCA downregulation could decrease apoptosis and induce autophagy in PD. In conclusion, the downregulation of circSNCA by PPX treatment reduced cell apoptosis and promoted cell autophagy in PD via a mechanism that served as a miR-7 sponge to upregulate SNCA. |
| Author | Sun, Yajuan Zhang, Haina Wang, Libo Sang, Qiuling Liu, Xiaoyang Qi, Ling Wang, Weiyao Sun, Wenping |
| Author_xml | – sequence: 1 givenname: Qiuling surname: Sang fullname: Sang, Qiuling organization: Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China – sequence: 2 givenname: Xiaoyang surname: Liu fullname: Liu, Xiaoyang organization: Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China – sequence: 3 givenname: Libo surname: Wang fullname: Wang, Libo organization: Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China – sequence: 4 givenname: Ling surname: Qi fullname: Qi, Ling organization: Department of Pathophysiology, Jilin Medical University, Jilin, Jilin 132013, China – sequence: 5 givenname: Wenping surname: Sun fullname: Sun, Wenping organization: Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China – sequence: 6 givenname: Weiyao surname: Wang fullname: Wang, Weiyao organization: Department of Pathophysiology, Jilin Medical University, Jilin, Jilin 132013, China – sequence: 7 givenname: Yajuan surname: Sun fullname: Sun, Yajuan organization: Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China – sequence: 8 givenname: Haina surname: Zhang fullname: Zhang, Haina organization: Department of Rehabilitation, the Second Hospital of Jilin University, Changchun, Jilin 130041, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29953413$$D View this record in MEDLINE/PubMed |
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| Title | CircSNCA downregulation by pramipexole treatment mediates cell apoptosis and autophagy in Parkinson's disease by targeting miR-7 |
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