Glycosaminoglycan content of glomerular and tubular basement membranes of various mammalian species

A spectrophotometric assay was applied for quantitation of sulfated glycosaminoglycans in digested renal basement membranes of six mammalian species. The conditions of digestion and the accuracy of the assay were evaluated. Papain digestion and alkaline treatment appeared to be most effective for so...

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Bibliographic Details
Published in:Biochimica et biophysica acta Vol. 838; no. 1; p. 144
Main Authors: Reubsaet, F A, Langeveld, J P, Veerkamp, J H
Format: Journal Article
Language:English
Published: Netherlands 28.01.1985
Subjects:
Animals
Basement Membrane - analysis
Cattle
Deoxycholic Acid
Glycosaminoglycans - analysis
Horses
Humans
Hydrogen-Ion Concentration
Kidney Glomerulus - analysis
Kidney Tubules - analysis
Papain
Rabbits
Sheep
Solubility
Sonication
Spectrophotometry
Swine
ISSN:0006-3002
Online Access:Get more information
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Summary:A spectrophotometric assay was applied for quantitation of sulfated glycosaminoglycans in digested renal basement membranes of six mammalian species. The conditions of digestion and the accuracy of the assay were evaluated. Papain digestion and alkaline treatment appeared to be most effective for solubilization. Basement membrane preparations obtained by sonication contained more glycosaminoglycans than those isolated by detergent treatment. Glomerular basement membranes had generally a higher glycosaminoglycan content than tubular basement membranes.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-3002
DOI:10.1016/0304-4165(85)90260-0