Glycosaminoglycans of arterial basement membrane-like material from cultured rabbit aortic myomedial cells

Arterial basement membrane-like material was prepared by a sonication-differential centrifugation technique from cultures of rabbit aortic myomedial cells after metabolic labelling with [35S]sulphate and [3H]glucosamine. Labelled glycosaminoglycans were obtained from isolated basement membrane-like...

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Vydané v:Biochimica et biophysica acta Ročník 798; číslo 2; s. 276
Hlavní autori: Heickendorff, L, Ledet, T
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands 10.04.1984
Predmet:
Animals
Aorta, Thoracic - analysis
Basement Membrane - analysis
Cells, Cultured
Chromatography, DEAE-Cellulose
Glycosaminoglycans - isolation & purification
Rabbits
Sulfur Radioisotopes
Tritium
ISSN:0006-3002
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Shrnutí:Arterial basement membrane-like material was prepared by a sonication-differential centrifugation technique from cultures of rabbit aortic myomedial cells after metabolic labelling with [35S]sulphate and [3H]glucosamine. Labelled glycosaminoglycans were obtained from isolated basement membrane-like material by proteinase digestion and gel filtration. Glycosaminoglycans were identified by a combination of Sephadex G-50 chromatography and sequential degradation with nitrous acid, Streptomyces hyaluronidase, testicular hyaluronidase and chondroitinase ABC. The data showed that heparan sulphate and chondroitin sulphate were the predominant glycosaminoglycans of myomedial basement membrane-like material. Heparan sulphate accounted for about 55% of [3H]glucosamine-labelled glycosaminoglycans. In addition small amounts of hyaluronic acid was present. Only trace amounts of dermatan sulphate was found. The glycosaminoglycans were analysed by DEAE-cellulose chromatography. Two major peaks were found in the chromatogram consistent with the predominance of heparan sulphate and chondroitin sulphate.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-3002
DOI:10.1016/0304-4165(84)90316-7