A flow-cytometric method for the separation and quantitation of normal and apoptotic thymocytes

Using flow cytometry, we describe a method for separating and quantifying normal and apoptotic thymocytes. Apoptosis was induced in isolated thymocytes from immature rats by treatment with the glucocorticoid dexamethasone or the antitumor agent etoposide. Subsequent incubation with the vital bisbenz...

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Bibliographic Details

Published in:	Analytical biochemistry Vol. 204; no. 2; p. 351
Main Authors:	Sun, X M, Snowden, R T, Skilleter, D N, Dinsdale, D, Ormerod, M G, Cohen, G M
Format:	Journal Article
Language:	English
Published:	United States 01.08.1992
Subjects:	Animals Apoptosis - drug effects Benzimidazoles Cell Separation - methods Dexamethasone - pharmacology DNA Damage Etoposide - pharmacology Flow Cytometry - methods Microscopy, Electron Rats Thymus Gland - cytology Thymus Gland - drug effects
ISSN:	0003-2697
Online Access:	Get more information
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