Evaluation of inter‐individual differences in gut bacterial isoflavone bioactivation in humans by PCR‐based targeting of genes involved in equol formation

Aim To identify human subjects harbouring intestinal bacteria that bioactivate daidzein to equol using a targeted PCR‐based approach. Methods and Results In a pilot study including 17 human subjects, equol formation was determined in faecal slurries. In parallel, faecal DNA was amplified by PCR usin...

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Bibliographic Details
Published in:	Journal of applied microbiology Vol. 124; no. 1; pp. 220 - 231
Main Authors:	Braune, A., Blaut, M.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01.01.2018
Subjects:	Adult Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biodiversity Biological activity Daidzein Deoxyribonucleic acid digestive system dihydrodaidzein reductase DNA equol Equol - metabolism Feces - microbiology Female Forming Gastrointestinal Microbiome Gastrointestinal Tract - microbiology Genes human intestinal microbiota Human subjects Humans intestinal microorganisms Intestine isoflavone Isoflavones Isoflavones - metabolism Male metabolism Oxidoreductases - genetics Oxidoreductases - metabolism Pilot Projects Polymerase chain reaction Polymerase Chain Reaction - methods Primers Reductase Slackia Slurries tetrahydrodaidzein reductase daidzein equol isoflavone human intestinal microbiota dihydrodaidzein reductase tetrahydrodaidzein reductase
ISSN:	1364-5072, 1365-2672, 1365-2672
Online Access:	Get full text
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