Antimicrobial susceptibility testing: currently used methods and devices and the near future in clinical practice

Summary Rapid identification of pathogen and its resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment are essential for correct patient outcomes. Conventional detection methods of bacterial resistance, such as disc diffusion, broth microdilution and automated instrum...

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Bibliographic Details
Published in:Journal of applied microbiology Vol. 129; no. 4; pp. 806 - 822
Main Authors: Benkova, M., Soukup, O., Marek, J.
Format: Journal Article
Language:English
Published: England Oxford University Press 01.10.2020
Subjects:
Anti-Bacterial Agents - pharmacology
antibiotic resistance
antibiotic susceptibility test
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
antimicrobial properties
antimicrobial susceptibility testing
automation
Bacteria - drug effects
Bacterial Infections - diagnosis
Bacterial Infections - microbiology
Clinical microbiology
desorption
dilution method
Drug resistance
Humans
Ionization
Ions
Laboratories
MALDI‐TOF MS
Mass spectrometry
Mass spectroscopy
Microbiological Techniques - instrumentation
Microbiological Techniques - trends
Microbiology
microcalorimetry
microdroplet
microfluidic
microfluidic technology
Microfluidics
minimum inhibitory concentration
pathogens
patients
dilution method
microcalorimetry
MALDI-TOF MS
antimicrobial susceptibility testing
microdroplet
antibiotic susceptibility test
microfluidic
ISSN:1364-5072, 1365-2672, 1365-2672
Online Access:Get full text
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Summary:Summary Rapid identification of pathogen and its resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment are essential for correct patient outcomes. Conventional detection methods of bacterial resistance, such as disc diffusion, broth microdilution and automated instruments, are constantly widely used and primarily standardized. Nevertheless, the results cannot be obtained earlier than 48 h after receiving a sample, which may lead to prolonged use or overuse of broad‐spectrum antibiotics. Hence, there is a drive to develop and introduce novel, faster, standardized, sensitive and specific methods with reliable results into routine microbiological laboratory practice. Recently developed matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has been introduced in recent years into laboratory practice, and methods based on microfluidics and microdroplets might be introduced in the near future. This review is focused on the methods and instruments in use both currently and in the foreseeable future, applicable to determine antimicrobial efficacy in clinical microbiology laboratories.
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ISSN:1364-5072
1365-2672
1365-2672
DOI:10.1111/jam.14704