The Potential of High-Throughput DNA Sequencing of the Paranasal Sinus Microbiome in Diagnosing Odontogenic Sinusitis

High-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to evaluate the use of high-throughput DNA sequencing to diagnose sinusitis of odontogenic origin. Case series with chart review. Single tertiary...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Otolaryngology-head and neck surgery Ročník 161; číslo 6; s. 1043
Hlavní autori:	Haider, Asad A, Marino, Michael J, Yao, William C, Citardi, Martin J, Luong, Amber U
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	England 01.12.2019
Predmet:	Adult Female High-Throughput Nucleotide Sequencing Humans Male Maxillary Sinusitis - diagnostic imaging Maxillary Sinusitis - microbiology Maxillary Sinusitis - therapy Microbiota Paranasal Sinuses - microbiology Retrospective Studies RNA, Bacterial - isolation & purification Sensitivity and Specificity Tomography, X-Ray Computed odontogenic sinusitis sinusitis microbiome DNA sequencing
ISSN:	1097-6817, 1097-6817
On-line prístup:	Zistit podrobnosti o prístupe
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Abstract	High-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to evaluate the use of high-throughput DNA sequencing to diagnose sinusitis of odontogenic origin. Case series with chart review. Single tertiary care academic medical center. A chart review was performed of DNA sequencing results from the sinus aspirates obtained under endoscopic visualization in 142 patients with sinusitis. The identification of any potentially pathogenic bacteria associated with oral flora in a sample was classified as a positive result for sinusitis of odontogenic etiology. The sensitivity, specificity, and predictive values of using high-throughput DNA sequencing to diagnose sinusitis of odontogenic etiology were determined, with the patient's computed tomography sinus scan as the reference standard. On computed tomography scans, an odontogenic source was determined by the presence of a periapical lucency perforating the schneiderian membrane. Seven of the 142 patients enrolled in this study had an odontogenic source based on computed tomography scans. Relative to this reference standard, high-throughput DNA sequencing produced a sensitivity of 85.7% (95% CI, 42.1%-99.6%), a specificity of 81.5% (95% CI, 73.9%-87.6%), a positive predictive value of 19.4% (95% CI, 13.1%-27.7%), and a negative predictive value of 99.1% (95% CI, 94.7%-99.9%). This study supports the use of high-throughput DNA sequencing in supplementing other methods of investigation for identifying an odontogenic etiology of sinusitis.
AbstractList	High-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to evaluate the use of high-throughput DNA sequencing to diagnose sinusitis of odontogenic origin.OBJECTIVEHigh-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to evaluate the use of high-throughput DNA sequencing to diagnose sinusitis of odontogenic origin.Case series with chart review.STUDY DESIGNCase series with chart review.Single tertiary care academic medical center.SETTINGSingle tertiary care academic medical center.A chart review was performed of DNA sequencing results from the sinus aspirates obtained under endoscopic visualization in 142 patients with sinusitis. The identification of any potentially pathogenic bacteria associated with oral flora in a sample was classified as a positive result for sinusitis of odontogenic etiology. The sensitivity, specificity, and predictive values of using high-throughput DNA sequencing to diagnose sinusitis of odontogenic etiology were determined, with the patient's computed tomography sinus scan as the reference standard. On computed tomography scans, an odontogenic source was determined by the presence of a periapical lucency perforating the schneiderian membrane.SUBJECTS AND METHODSA chart review was performed of DNA sequencing results from the sinus aspirates obtained under endoscopic visualization in 142 patients with sinusitis. The identification of any potentially pathogenic bacteria associated with oral flora in a sample was classified as a positive result for sinusitis of odontogenic etiology. The sensitivity, specificity, and predictive values of using high-throughput DNA sequencing to diagnose sinusitis of odontogenic etiology were determined, with the patient's computed tomography sinus scan as the reference standard. On computed tomography scans, an odontogenic source was determined by the presence of a periapical lucency perforating the schneiderian membrane.Seven of the 142 patients enrolled in this study had an odontogenic source based on computed tomography scans. Relative to this reference standard, high-throughput DNA sequencing produced a sensitivity of 85.7% (95% CI, 42.1%-99.6%), a specificity of 81.5% (95% CI, 73.9%-87.6%), a positive predictive value of 19.4% (95% CI, 13.1%-27.7%), and a negative predictive value of 99.1% (95% CI, 94.7%-99.9%).RESULTSSeven of the 142 patients enrolled in this study had an odontogenic source based on computed tomography scans. Relative to this reference standard, high-throughput DNA sequencing produced a sensitivity of 85.7% (95% CI, 42.1%-99.6%), a specificity of 81.5% (95% CI, 73.9%-87.6%), a positive predictive value of 19.4% (95% CI, 13.1%-27.7%), and a negative predictive value of 99.1% (95% CI, 94.7%-99.9%).This study supports the use of high-throughput DNA sequencing in supplementing other methods of investigation for identifying an odontogenic etiology of sinusitis.CONCLUSIONThis study supports the use of high-throughput DNA sequencing in supplementing other methods of investigation for identifying an odontogenic etiology of sinusitis. High-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to evaluate the use of high-throughput DNA sequencing to diagnose sinusitis of odontogenic origin. Case series with chart review. Single tertiary care academic medical center. A chart review was performed of DNA sequencing results from the sinus aspirates obtained under endoscopic visualization in 142 patients with sinusitis. The identification of any potentially pathogenic bacteria associated with oral flora in a sample was classified as a positive result for sinusitis of odontogenic etiology. The sensitivity, specificity, and predictive values of using high-throughput DNA sequencing to diagnose sinusitis of odontogenic etiology were determined, with the patient's computed tomography sinus scan as the reference standard. On computed tomography scans, an odontogenic source was determined by the presence of a periapical lucency perforating the schneiderian membrane. Seven of the 142 patients enrolled in this study had an odontogenic source based on computed tomography scans. Relative to this reference standard, high-throughput DNA sequencing produced a sensitivity of 85.7% (95% CI, 42.1%-99.6%), a specificity of 81.5% (95% CI, 73.9%-87.6%), a positive predictive value of 19.4% (95% CI, 13.1%-27.7%), and a negative predictive value of 99.1% (95% CI, 94.7%-99.9%). This study supports the use of high-throughput DNA sequencing in supplementing other methods of investigation for identifying an odontogenic etiology of sinusitis.
Author	Luong, Amber U Yao, William C Marino, Michael J Citardi, Martin J Haider, Asad A
Author_xml	– sequence: 1 givenname: Asad A surname: Haider fullname: Haider, Asad A organization: Department of Otorhinolaryngology-Head and Neck Surgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA – sequence: 2 givenname: Michael J surname: Marino fullname: Marino, Michael J organization: Department of Otorhinolaryngology, Mayo Clinic, Phoenix, Arizona, USA – sequence: 3 givenname: William C surname: Yao fullname: Yao, William C organization: Department of Otorhinolaryngology-Head and Neck Surgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA – sequence: 4 givenname: Martin J surname: Citardi fullname: Citardi, Martin J organization: Department of Otorhinolaryngology-Head and Neck Surgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA – sequence: 5 givenname: Amber U surname: Luong fullname: Luong, Amber U organization: McGovern Medical School, The University of Texas Health Science Center at Houston, Center for Immunology and Autoimmune Diseases, Institute of Molecular Medicine, Houston, Texas, USA
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31382814$$D View this record in MEDLINE/PubMed
BookMark	eNpNkDtPwzAUhS1URB-wMyGPLAFfJ_FjrFqgSIUitcyR4ziJUWqXOBn496RqkZjuGb5zpO9O0ch5ZxC6BfIAwPkjAZmkUgqQgjEm6QWaAJE8YgL46F8eo2kIX4QQxji_QuMYYkEFJBPU72qDP3xnXGdVg32JV7aqo13d-r6qD32Hl-9zvDXfvXHauupIdMeKapVTYahsresDfrO69bn1e4Otw0urKufDkd8U3nW-Ms7qE2o7G67RZamaYG7Od4Y-n592i1W03ry8LubrSMeCxVGaC5MSDrmWWkOpBmeZS1OWCY1TItKClbFmMNgAEMpUWnIQrIgLlSudaE5n6P60e2j9YBC6bG-DNk2jnPF9yChlQiYQJ2xA785on-9NkR1au1ftT_b3KvoL2uRteg
CitedBy_id	crossref_primary_10_1016_j_amjoto_2021_102925 crossref_primary_10_1155_2021_9997180 crossref_primary_10_1177_19458924241259333 crossref_primary_10_1111_eve_14137 crossref_primary_10_1177_1945892420941702 crossref_primary_10_1177_1945892420976766 crossref_primary_10_37219_2528_8253_2024_2_50 crossref_primary_10_1007_s00405_022_07284_z crossref_primary_10_1016_j_otc_2022_09_014 crossref_primary_10_1177_01945998211026268 crossref_primary_10_1002_alr_22777 crossref_primary_10_1038_s41368_024_00278_z
ContentType	Journal Article
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1177/0194599819866692
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Medicine
EISSN	1097-6817
ExternalDocumentID	31382814
Genre	Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	--- --K -TM .55 .GJ 01A 0R~ 123 1B1 1CY 1OB 1OC 1~5 29O 354 4.4 4G. 53G 54M 5RE 5VS 7-5 71M 8F7 AABNK AABOD AACKU AADTT AAEDT AAHQN AAIKJ AAIPD AAJQC AALRI AAMGE AAMNL AANLZ AANSI AAPEO AAQFI AAQQG AAQQT AAQXK AARDL AARIX AATBZ AAWTL AAXUO AAYCA ABAFQ ABAWP ABCCA ABEIX ABFNM ABFWQ ABJNI ABKRH ABMAC ABNCE ABQKF ABQWH ABQXT ABVFX ABWVN ACAEH ACDSZ ACDXX ACGFS ACGZU ACNXM ACOFE ACRPL ACSBE ACSIQ ACTQU ACUIR ACXQS ADBBV ADBTR ADMUD ADNMO ADUCT ADZZY AEIGN AEKER AENEX AEQLS AERKM AEUHG AEUIJ AEUYR AEWDL AEWHI AFEET AFFNX AFFPM AFKBI AFKRG AFOSN AFUIA AFWVQ AHBTC AIEWD AIOMO AITUG AITYG AJUZI ALMA_UNASSIGNED_HOLDINGS ARTOV ASPBG AUTPY AVWKF AYAKG AZFZN B8M BDDNI BSEHC BYIEH C45 CAG CBRKF CDWPY CFDXU CGR COF CS3 CUY CVF DC- DCZOG DD0 DOPDO DU5 DV7 EBS ECM EIF EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FNPLU G-Q GROUPED_SAGE_PREMIER_JOURNAL_COLLECTION H13 HF~ HGLYW HJTMK HVGLF HZ~ IHE J8X K.F M41 M4V MEWTI NPM NQ- O9- OHH OVD OZT P.B Q1R R2- RIG ROL RPZ S01 SAMSI SAUOL SDG SDP SEL SEW SFC SJN SSZ SUPJJ TEORI UDS UGJ WH7 WXSBR X7M ZGI ZONMY ZPPRI ZRKOI ZSSAH ZXP ZY1 7X8 AAMMB ADEBD AEFGJ AGXDD AGYGG AIDQK AIDYY LH4 SCNPE ~HD
ID	FETCH-LOGICAL-c3863-5b8e5071bc9cc1fa1179b9eff4235085d6f3c6131311026a5f7186d3dabac4c72
IEDL.DBID	7X8
ISICitedReferencesCount	12
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000481061600001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1097-6817
IngestDate	Sun Sep 28 11:57:00 EDT 2025 Wed Feb 19 02:30:31 EST 2025
IsPeerReviewed	true
IsScholarly	true
Issue	6
Keywords	odontogenic sinusitis sinusitis microbiome DNA sequencing
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c3863-5b8e5071bc9cc1fa1179b9eff4235085d6f3c6131311026a5f7186d3dabac4c72
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
PMID	31382814
PQID	2268941346
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_2268941346 pubmed_primary_31382814
PublicationCentury	2000
PublicationDate	2019-12-00 20191201
PublicationDateYYYYMMDD	2019-12-01
PublicationDate_xml	– month: 12 year: 2019 text: 2019-12-00
PublicationDecade	2010
PublicationPlace	England
PublicationPlace_xml	– name: England
PublicationTitle	Otolaryngology-head and neck surgery
PublicationTitleAlternate	Otolaryngol Head Neck Surg
PublicationYear	2019
SSID	ssj0006677
Score	2.3480842
Snippet	High-throughput DNA sequencing of the paranasal sinus microbiome has potential in the diagnosis and treatment of sinusitis. The objective of this study is to...
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	1043
SubjectTerms	Adult Female High-Throughput Nucleotide Sequencing Humans Male Maxillary Sinusitis - diagnostic imaging Maxillary Sinusitis - microbiology Maxillary Sinusitis - therapy Microbiota Paranasal Sinuses - microbiology Retrospective Studies RNA, Bacterial - isolation & purification Sensitivity and Specificity Tomography, X-Ray Computed
Title	The Potential of High-Throughput DNA Sequencing of the Paranasal Sinus Microbiome in Diagnosing Odontogenic Sinusitis
URI	https://www.ncbi.nlm.nih.gov/pubmed/31382814 https://www.proquest.com/docview/2268941346
Volume	161
WOSCitedRecordID	wos000481061600001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1La8MwDDbbOsYuez-6Fx7satrUjpOcxlhXdmlWaAe9BT9i6GFJtzT7_ZPilJ4Gg11yiQTGlqVPlvhEyAMXYawSaVm_bxUTANGZ6oeKaaeTHC6fjgLbDJuI0jSez5NJ--BWtW2Va5_YOGpbGnwj7wFMiBPwuEI-Lj8ZTo3C6mo7QmObdDhAGbTqaL5hC5eymbyIRVYm4yDalCl7gGxECKkG5NwA4JtC6C8Aswk0o8P_LvGIHLQQkz55mzgmW3lxQvbGbRH9lNRgGnRSrrBPCORKR7HZg838xJ5lvaLD9IlOfY81RDaUWKGKgrimKlCZLoq6ouOFJ3H6yOmioEPfs4fybxZJEcAyF8aLIm_SGXkfvcyeX1k7fYEZHkvOQh3nCBa1SYwJnELuODg_5wCAAaoLrXTcABhAvh5I5FToIMxJy63SyggTDc7JTlEW-SWhPJTCwm_jdCiCxCqrteaDXApnpAuSLrlfb2gG1o0lC1XkZV1lmy3tkgt_KtnS03BkHOkT40Bc_UH7muwD0kl8H8oN6Ti42_kt2TXfsAFfd43ZwDedjH8AIB3NKg
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Potential+of+High-Throughput+DNA+Sequencing+of+the+Paranasal+Sinus+Microbiome+in+Diagnosing+Odontogenic+Sinusitis&rft.jtitle=Otolaryngology-head+and+neck+surgery&rft.au=Haider%2C+Asad+A&rft.au=Marino%2C+Michael+J&rft.au=Yao%2C+William+C&rft.au=Citardi%2C+Martin+J&rft.date=2019-12-01&rft.issn=1097-6817&rft.eissn=1097-6817&rft.volume=161&rft.issue=6&rft.spage=1043&rft_id=info:doi/10.1177%2F0194599819866692&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-6817&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-6817&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-6817&client=summon