Importance of anaerobic ammonium oxidation as a nitrogen removal pathway in freshwater marsh sediments

Aims To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. Methods and Results The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is...

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Published in:Journal of applied microbiology Vol. 125; no. 5; pp. 1423 - 1434
Main Authors: Shen, L.‐D., Liu, X., Wu, H.‐S.
Format: Journal Article
Language:English
Published: England Oxford University Press 01.11.2018
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ISSN:1364-5072, 1365-2672, 1365-2672
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Abstract Aims To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. Methods and Results The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina‐based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox‐related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 104 to 1·58 × 105 copies per g sediment with little temporal variation. The potential anammox rates measured by 15N‐stable isotope pairing technique were 0·78–5·37 nmol N g−1 sediment per h, accounting for 4·3–38·5% of total sediment dinitrogen gas (N2) production. Both the anammox activity and its contribution to N2 production were sensitive to temporal variation and correlated well with the sediment NO3− content. To further examine the nitrogen removal potential via anammox, batch culture was set‐up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g−1 sediment per h and 2·86 × 105 to 6·58 × 105 copies per g sediment respectively. Conclusions Our results revealed the great potential of anammox in nitrogen removal in freshwater marshes. Significance and Impact of the Study This is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
AbstractList AimsTo explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes.Methods and ResultsThe 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina‐based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox‐related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 104 to 1·58 × 105 copies per g sediment with little temporal variation. The potential anammox rates measured by 15N‐stable isotope pairing technique were 0·78–5·37 nmol N g−1 sediment per h, accounting for 4·3–38·5% of total sediment dinitrogen gas (N2) production. Both the anammox activity and its contribution to N2 production were sensitive to temporal variation and correlated well with the sediment NO3− content. To further examine the nitrogen removal potential via anammox, batch culture was set‐up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g−1 sediment per h and 2·86 × 105 to 6·58 × 105 copies per g sediment respectively.ConclusionsOur results revealed the great potential of anammox in nitrogen removal in freshwater marshes.Significance and Impact of the StudyThis is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
AIMS: To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. METHODS AND RESULTS: The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina‐based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox‐related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 10⁴ to 1·58 × 10⁵ copies per g sediment with little temporal variation. The potential anammox rates measured by ¹⁵N‐stable isotope pairing technique were 0·78–5·37 nmol N g⁻¹ sediment per h, accounting for 4·3–38·5% of total sediment dinitrogen gas (N₂) production. Both the anammox activity and its contribution to N₂ production were sensitive to temporal variation and correlated well with the sediment NO₃⁻ content. To further examine the nitrogen removal potential via anammox, batch culture was set‐up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g⁻¹ sediment per h and 2·86 × 10⁵ to 6·58 × 10⁵ copies per g sediment respectively. CONCLUSIONS: Our results revealed the great potential of anammox in nitrogen removal in freshwater marshes. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes.AIMSTo explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes.The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina-based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox-related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 104 to 1·58 × 105 copies per g sediment with little temporal variation. The potential anammox rates measured by 15 N-stable isotope pairing technique were 0·78-5·37 nmol N g-1 sediment per h, accounting for 4·3-38·5% of total sediment dinitrogen gas (N2 ) production. Both the anammox activity and its contribution to N2 production were sensitive to temporal variation and correlated well with the sediment NO3 - content. To further examine the nitrogen removal potential via anammox, batch culture was set-up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g-1 sediment per h and 2·86 × 105 to 6·58 × 105 copies per g sediment respectively.METHODS AND RESULTSThe 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina-based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox-related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 104 to 1·58 × 105 copies per g sediment with little temporal variation. The potential anammox rates measured by 15 N-stable isotope pairing technique were 0·78-5·37 nmol N g-1 sediment per h, accounting for 4·3-38·5% of total sediment dinitrogen gas (N2 ) production. Both the anammox activity and its contribution to N2 production were sensitive to temporal variation and correlated well with the sediment NO3 - content. To further examine the nitrogen removal potential via anammox, batch culture was set-up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g-1 sediment per h and 2·86 × 105 to 6·58 × 105 copies per g sediment respectively.Our results revealed the great potential of anammox in nitrogen removal in freshwater marshes.CONCLUSIONSOur results revealed the great potential of anammox in nitrogen removal in freshwater marshes.This is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.SIGNIFICANCE AND IMPACT OF THE STUDYThis is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
Aims To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. Methods and Results The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina‐based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox‐related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 104 to 1·58 × 105 copies per g sediment with little temporal variation. The potential anammox rates measured by 15N‐stable isotope pairing technique were 0·78–5·37 nmol N g−1 sediment per h, accounting for 4·3–38·5% of total sediment dinitrogen gas (N2) production. Both the anammox activity and its contribution to N2 production were sensitive to temporal variation and correlated well with the sediment NO3− content. To further examine the nitrogen removal potential via anammox, batch culture was set‐up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g−1 sediment per h and 2·86 × 105 to 6·58 × 105 copies per g sediment respectively. Conclusions Our results revealed the great potential of anammox in nitrogen removal in freshwater marshes. Significance and Impact of the Study This is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. The 16S rRNA gene sequences of Candidatus Kuenenia and Candidatus Brocadia were simultaneously detected in the sediment of freshwater marshes of Green Bay Wetland that is located in Eastern China by using Illumina-based sequencing of the total bacterial 16S rRNA genes, and Candidatus Brocadia comprised more than 80% of the total anammox-related sequences. The abundance of anammox bacteria was determined by quantitative PCR on their hydrazine synthase (hzs) genes, which ranged from 3·13 × 10 to 1·58 × 10 copies per g sediment with little temporal variation. The potential anammox rates measured by N-stable isotope pairing technique were 0·78-5·37 nmol N g sediment per h, accounting for 4·3-38·5% of total sediment dinitrogen gas (N ) production. Both the anammox activity and its contribution to N production were sensitive to temporal variation and correlated well with the sediment NO content. To further examine the nitrogen removal potential via anammox, batch culture was set-up to enrich anammox bacteria from the marsh sediments. Both the activity and abundance of anammox bacteria increased significantly after 6 months of incubation, varying from 61·6 to 95·8 nmol N g sediment per h and 2·86 × 10 to 6·58 × 10 copies per g sediment respectively. Our results revealed the great potential of anammox in nitrogen removal in freshwater marshes. This is the first study to show the anammox activity and its temporal variation in freshwater marsh sediments, which improved our understanding of nitrogen removal mechanisms in freshwater marshes.
Author Shen, L.‐D.
Liu, X.
Wu, H.‐S.
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Issue 5
Keywords anaerobic ammonium oxidation
activity
freshwater marshes
temporal variation
nitrogen removal
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Snippet Aims To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. Methods and Results The 16S rRNA gene sequences...
To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. The 16S rRNA gene sequences of Candidatus Kuenenia and...
AimsTo explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes.Methods and ResultsThe 16S rRNA gene sequences of...
To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes.AIMSTo explore the role of anaerobic ammonium oxidation...
AIMS: To explore the role of anaerobic ammonium oxidation (anammox) in nitrogen removal in freshwater marshes. METHODS AND RESULTS: The 16S rRNA gene sequences...
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SubjectTerms Abundance
activity
Ammonia-oxidizing bacteria
Ammonium
Ammonium Compounds - metabolism
anaerobic ammonium oxidation
Anaerobic bacteria
Anaerobiosis
Bacteria
Batch culture
Candidatus Brocadia
Candidatus Kuenenia
China
Denitrification
Fresh water
Fresh Water - chemistry
Fresh Water - microbiology
freshwater
freshwater marshes
Gene sequencing
Genes
Geologic Sediments - chemistry
Geologic Sediments - microbiology
Hydrazine
Hydrazines
isotopes
Marshes
nitrates
nitrifying bacteria
Nitrogen
Nitrogen - analysis
Nitrogen - metabolism
Nitrogen removal
nucleotide sequences
Oxidation
Oxidation-Reduction
Phylogeny
Planctomycetales - genetics
Planctomycetales - isolation & purification
Planctomycetales - metabolism
quantitative polymerase chain reaction
ribosomal RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Salt
Sediments
Soil erosion
Stable isotopes
temporal variation
Temporal variations
Wetlands
Title Importance of anaerobic ammonium oxidation as a nitrogen removal pathway in freshwater marsh sediments
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fjam.14042
https://www.ncbi.nlm.nih.gov/pubmed/29979471
https://www.proquest.com/docview/2120018712
https://www.proquest.com/docview/2066479204
https://www.proquest.com/docview/2153611099
Volume 125
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